Zobrazeno 1 - 10
of 66
pro vyhledávání: '"Abdurashitov MA"'
Autor:
Abdurashitov Ma, S. Kh. Degtyarev
Publikováno v:
Russian Journal of Genetics. 53:1-8
During the last decades, site-specific DNA endonucleases have served as a key instrument to study primary structure of DNA and genetic engineering. Here, we describe examples of these enzyme uses in genome-wide analysis of human DNA including restric
Autor:
Natalia A. Smetannikova, r G. Akishev, Yuriy D. Ermolaev, N. A. Netesova, Boris S. Malyshev, Alexey E. Sazonov, Alexey A. Evdokimov, Sergey Kh. Degtyarev, Evgeniya S. Davidovich, Karpov Ab, Abdurashitov Ma, Ravil M. Tahauov, Alex, Vitaliy V. Kuznetsov, Vladimir V. Fedotov
Publikováno v:
Biology and Medicine.
Hypermethylation of the gene regulatory regions is documented for many cancer diseases. Such an aberrant DNA methylation in cancer cells is catalyzed by DNA methyltransferases Dnmt3a and Dnmt3b, which predominantly recognize and methylate RCGY sequen
Publikováno v:
Molecular Biology. 44:606-615
The M1.Bst19I DNA-methyltransferase gene from restriction-modification system Bst19I (recognition sequence 5'-GCATC-3') in Bacillus stearothermophilus 19 has been cloned in the expressing vector pJW, that carries a tandem of thermo inducible promoter
Autor:
S. Kh. Degtyarev, D. A. Gonchar, E.V. Kileva, Abdurashitov Ma, Dmitriy A. Shagin, S. S. Okhapkina
Publikováno v:
Molecular Biology. 41:438-444
The nucleotide sequence was established for the operon of the Sse9I type II restriction-modification system of Sporosarcina species 9D. The enzymes of the Sse9I system recognize the 5′-AATT-3′ tetranucleotide. The operon includes three genes, sse
Publikováno v:
Molecular Biology. 39:840-844
The nucleotide sequence of Bacillus stearothermophilus SE-589 DNA fragment including an operon for the site-specific nicking-modification (NM) system with a gene for BstSEI nicking endonuclease (nickase) has been determined. An analysis of the region
Publikováno v:
Molecular Biology. 38:850-856
The Streptococcus faecalis ND547 and Bacillus stearothermophilus 19 genes that code for DNA methyltransferases (MTases, M.) of restriction–modification (RM) systems with the same recognition sequence, 5′-GCATC-3′ were cloned and sequenced. The
Autor:
S. Kh. Degtyarev, D. A. Gonchar, L. N. Golikova, N. A. Netesova, Abdurashitov Ma, Yu. G. Kashirina, Valery A Chernukhin
Publikováno v:
Biochemistry (Moscow). 68:967-975
The BstF5I restriction–modification system from Bacillus stearothermophilus F5 includes four site-specific DNA methyltransferases, thus differing from all known restriction–modification systems. Here we demonstrated for the first time that one ba
Autor:
S. Kh. Degtyarev, D. A. Gonchar, L. N. Golikova, N. A. Netesova, Abdurashitov Ma, S. S. Okhapkina
Publikováno v:
Molecular Biology. 37:524-528
The nucleotide sequence was established for the full-length Flavobacterium aquatile operon coding for the FauI restriction–modification system. The operon is unusual in structure and has the gene order control protein / DNA methyltransferase A / re
Autor:
N. A. Netesova, S. V. Sosnovtsev, Abdurashitov Ma, E. V. Seregina, S. Kh. Degtyarev, L. N. Golikova, S. S. Okhapkina
Publikováno v:
Molecular Biology. 36:333-337
A fragment containing the SfeI restriction–modification system (RMS) operon was cloned from a Streptococcus faecalis SE72 plasmid. Nucleotide sequence analysis revealed its high (99.2%) homology to the operon for Lactococcus lactis subsp. cremoris
Autor:
Valery A Chernukhin, Victor N Tomilov, Abdurashitov Ma, Natalia G. Schostak, Sergey Kh. Degtyarev, Michael B. Evgen'ev, D. A. Gonchar, Zelentsova Es, Olga G. Zatsepina, Julia E Tomilova
Publikováno v:
BMC Genomics
Background Previously, we developed a simple method for carrying out a restriction enzyme analysis of eukaryotic DNA in silico, based on the known DNA sequences of the genomes. This method allows the user to calculate lengths of all DNA fragments tha