Zobrazeno 1 - 10
of 22
pro vyhledávání: '"Aaron Zhong"'
Publikováno v:
STAR Protocols, Vol 4, Iss 4, Pp 102583- (2023)
Summary: Prime editing introduces single-nucleotide polymorphism changes, small deletions, or insertions at a specific genome site without double-stranded DNA breaks or the need for the donor template. Here, we present a protocol to design, conduct,
Externí odkaz:
https://doaj.org/article/87cf5a4702cf411cbb913b9a4468708c
Autor:
Liuliu Yang, Yuling Han, Ting Zhou, Lauretta A. Lacko, Mohsan Saeed, Christina Tan, Ron Danziger, Jiajun Zhu, Zeping Zhao, Clare Cahir, Alice Maria Giani, Yang Li, Xue Dong, Dorota Moroziewicz, Daniel Paull, Zhengming Chen, Aaron Zhong, Scott A. Noggle, Charles M. Rice, Qibin Qi, Todd Evans, Shuibing Chen
Publikováno v:
iScience, Vol 26, Iss 7, Pp 107001- (2023)
Summary: Population-based genome-wide association studies (GWAS) normally require a large sample size, which can be labor intensive and costly. Recently, we reported a human induced pluripotent stem cell (hiPSC) array-based GWAS method, identifying N
Externí odkaz:
https://doaj.org/article/692df0b5246247cd9c258ce33dcea793
Autor:
Mu Li, Aaron Zhong, Youjun Wu, Mega Sidharta, Michael Beaury, Xiaolan Zhao, Lorenz Studer, Ting Zhou
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-12 (2022)
Li et al. report that co-delivering a dominant negative fragment of p53 (p53DD) greatly enhances precise editing efficiencies for prime editing and cytosine base editing in human pluripotent stem cells, without compromising the genome-wide safety.
Externí odkaz:
https://doaj.org/article/10a894ea600a4c1c8a1340e972626ca9
Autor:
Lei Tan, Lauretta A. Lacko, Ting Zhou, Delia Tomoiaga, Romulo Hurtado, Tuo Zhang, Ana Sevilla, Aaron Zhong, Christopher E Mason, Scott Noggle, Todd Evans, Heidi Stuhlmann, Robert E. Schwartz, Shuibing Chen
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-12 (2019)
Here, using human cells and mouse models, the authors show that Zika virus can infect preimplantation trophectoderm. Pre-implantation infection can affect nervous system development and survival of neural progenitors, and can result in miscarriage or
Externí odkaz:
https://doaj.org/article/cc5622b5dfc04f5d988bdfabb453be0c
Autor:
Jihui Ren, Essa M. Saied, Aaron Zhong, Justin Snider, Christian Ruiz, Christoph Arenz, Lina M. Obeid, Geoffrey D. Girnun, Yusuf A. Hannun
Publikováno v:
Journal of Lipid Research, Vol 59, Iss 11, Pp 2126-2139 (2018)
The generation of most sphingolipids (SPLs) starts with condensation between serine and an activated long-chain fatty acid catalyzed by serine palmitoyltransferase (SPT). SPT can also use other amino acids to generate small quantities of noncanonical
Externí odkaz:
https://doaj.org/article/65445f4d155840a09e159621ab2b385f
Publikováno v:
STAR Protocols, Vol 1, Iss 2, Pp 100052- (2020)
Summary: Reporter cell lines based on human pluripotent stem cells (hPSCs) are highly desirable for studying differentiation, lineage tracing, and target cell selection. However, several technical bottlenecks, such as DNA transduction, low homology r
Externí odkaz:
https://doaj.org/article/cd0b0f851e36444d948059ed889a39db
Autor:
Jihui Ren, Justin Snider, Michael V. Airola, Aaron Zhong, Nadia A. Rana, Lina M. Obeid, Yusuf A. Hannun
Publikováno v:
Journal of Lipid Research, Vol 59, Iss 1, Pp 162-170 (2018)
Serine palmitoyltransferase (SPT) catalyzes the rate-limiting step of condensation of L-serine and palmitoyl-CoA to form 3-ketodihydrosphingosine (3KDS). Here, we report a HPLC-ESI-MS/MS method to directly quantify 3KDS generated by SPT. With this te
Externí odkaz:
https://doaj.org/article/5b938cc4aebb4269bf430a0899eb3290
Autor:
Yujie Fan, James Hackland, Arianna Baggiolini, Lin Y. Hung, Huiyong Zhao, Paul Zumbo, Polina Oberst, Andrew P. Minotti, Emiliano Hergenreder, Sarah Najjar, Zixing Huang, Nelly M. Cruz, Aaron Zhong, Mega Sidharta, Ting Zhou, Elisa de Stanchina, Doron Betel, Richard M. White, Michael Gershon, Kara Gross Margolis, Lorenz Studer
Publikováno v:
Cell Stem Cell. 30:264-282.e9
Autor:
Robert E. Schwartz, Ana Sevilla, Romulo Hurtado, Lei Tan, Tuo Zhang, Aaron Zhong, Lauretta A. Lacko, Delia Tomoiaga, Ting Zhou, Todd Evans, Heidi Stuhlmann, Scott Noggle, Christopher E. Mason, Shuibing Chen
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-12 (2019)
Nature Communications
Dipòsit Digital de la UB
Universidad de Barcelona
Nature Communications
Dipòsit Digital de la UB
Universidad de Barcelona
Zika virus (ZIKV) infection results in an increased risk of spontaneous abortion and poor intrauterine growth although the underlying mechanisms remain undetermined. Little is known about the impact of ZIKV infection during the earliest stages of pre
Autor:
Aaron Zhong, Lina M. Obeid, Christian F. Ruiz, Christoph Arenz, Geoffrey D. Girnun, Essa M. Saied, Yusuf A. Hannun, Justin Snider, Jihui Ren
Publikováno v:
Journal of Lipid Research, Vol 59, Iss 11, Pp 2126-2139 (2018)
The generation of most sphingolipids (SPLs) starts with condensation between serine and an activated long-chain fatty acid catalyzed by serine palmitoyltransferase (SPT). SPT can also use other amino acids to generate small quantities of noncanonical