Zobrazeno 1 - 10
of 82
pro vyhledávání: '"Aaron L Lucius"'
Publikováno v:
eLife, Vol 13 (2024)
Escherichia coli ClpB and Saccharomyces cerevisiae Hsp104 are AAA+ motor proteins essential for proteome maintenance and thermal tolerance. ClpB and Hsp104 have been proposed to extract a polypeptide from an aggregate and processively translocate the
Externí odkaz:
https://doaj.org/article/8825c1b66a304efca97fe6a31dddd9b2
Autor:
Sahar Foroutannejad, Lydia L. Good, Changfan Lin, Zachariah I. Carter, Mahlet G. Tadesse, Aaron L. Lucius, Brian R. Crane, Rodrigo A. Maillard
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-15 (2023)
Characterizing folding pathways of large proteins that bind complex cofactors is challenging. The authors use optical tweezers to study the 542-residue FAD-binding lightsensor protein dCRY, identifying several intermediates and cofactor binding steps
Externí odkaz:
https://doaj.org/article/30dd6d9302b84ee08c76dff6d29b567b
Publikováno v:
STAR Protocols, Vol 4, Iss 2, Pp 102191- (2023)
Summary: Here we present an optimized protocol for monitoring and analyzing single nucleotide incorporation by RNA polymerases. This protocol describes the assembly of Saccharomyces cerevisiae RNA polymerase I elongation complexes in a promoter-indep
Externí odkaz:
https://doaj.org/article/1553052518ab4d608327e3c118cd1b8f
Publikováno v:
iScience, Vol 25, Iss 11, Pp 105306- (2022)
Summary: Eukaryotes express three nuclear RNA polymerases (Pols I, II, and III) that are essential for cell survival. Despite extensive investigation of the three Pols, significant knowledge gaps regarding their biochemical properties remain because
Externí odkaz:
https://doaj.org/article/0134558c08134004b14bd60fea5e3d26
Publikováno v:
Biochem Soc Trans
Numerous ATPases associated with diverse cellular activities (AAA+) proteins form hexameric, ring-shaped complexes that function via ATPase-coupled translocation of substrates across the central channel. Cryo-electron microscopy of AAA+ proteins proc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8af7afd6815e06a0223fced2a83999f0
https://doi.org/10.1042/bst20200350
https://doi.org/10.1042/bst20200350
Publikováno v:
Biochemistry. 62(1)
Eukaryotic RNA polymerase II (Pol II) is an essential enzyme that lies at the core of eukaryotic biology. Due to its pivotal role in gene expression, Pol II has been subjected to a substantial number of investigations. We aim to further our understan
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a9bd7e187573b084aad2f3d4e5fbafd8
https://pubmed.ncbi.nlm.nih.gov/36525636
https://pubmed.ncbi.nlm.nih.gov/36525636
Autor:
Ruth Q. Jacobs, Kaila B. Fuller, Stephanie L. Cooper, Zachariah I. Carter, Marikki Laiho, Aaron L. Lucius, David A. Schneider
Publikováno v:
Cancers; Volume 14; Issue 22; Pages: 5544
Cancer cells require robust ribosome biogenesis to maintain rapid cell growth during tumorigenesis. Because RNA polymerase I (Pol I) transcription of the ribosomal DNA (rDNA) is the first and rate-limiting step of ribosome biogenesis, it has emerged
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bd089e4eeb1a9e29e8a906b6834fa2bd
Publikováno v:
Frontiers in Molecular Biosciences, Vol 4 (2017)
Cellular proteostasis involves not only the expression of proteins in response to environmental needs, but also the timely repair or removal of damaged or unneeded proteins. AAA+ motor proteins are critically involved in these pathways. Here, we revi
Externí odkaz:
https://doaj.org/article/48b6bd477c5c4de7bac04341afb702ed
Publikováno v:
Biophys J
Eukaryotes express three DNA-dependent RNA polymerases (Pols) that are responsible for the entirety of cellular genomic expression. The three Pols have evolved to express specific cohorts of RNAs and thus have diverged both structurally and functiona
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::19f3e31a0b5197c32964d0cff071108c
https://doi.org/10.1016/j.bpj.2021.03.007
https://doi.org/10.1016/j.bpj.2021.03.007
Autor:
Gary M. Gray, Victoria L. Stanford, Aaron L. Lucius, Ivan Popov, Stephen H. Foulger, Timothy R. Totsch
Publikováno v:
Journal of Polymer Science. 59:139-145
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a7b1c846abafe0b76c02c8e68f7b9c7d
https://doi.org/10.1002/pol.20200561
https://doi.org/10.1002/pol.20200561