CR1/2 is an important suppressor of Adenovirus-induced innate immune responses and is required for induction of neutralizing antibodies

Autor: Nathaniel J. Schuldt, Mathew Bujold, Aaron J. Mcbride, Sergey S. Seregin, Andrea Amalfitano, Yasser A. Aldhamen, Daniel M. Appledorn, Sarah Godbehere

Publikováno v: Gene therapy

Human complement receptors 1 and 2 are well described as important regulators of innate and adaptive immune responses, having pivotal roles in regulating complement activation (CR1) and B cell maturation/survival. In contrast, the role of the murine

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9447dcb1a04383e9983f6bb3c4d8aa97
https://doi.org/10.1038/gt.2009.77

Reporter enzyme inhibitor study to aid assembly of orthogonal reporter gene assays

Autor: Pramod Pandey, Brian Webb, Natasha Karassina, Lyne Breault, Keith V. Wood, Aaron J. McBride, Pei-i Ho, Douglas S. Auld, Adam Hill, Fred Harbinski, Kimberley Yue, Janaki Narahari

Publikováno v: ACS chemical biology. 8(5)

Reporter gene assays (RGAs) are commonly used to measure biological pathway modulation by small molecules. Understanding how such compounds interact with the reporter enzyme is critical to accurately interpret RGA results. To improve our understandin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e1fd89d1dc77a7c01d937b987468c645
https://pubmed.ncbi.nlm.nih.gov/23485150

Transient pretreatment with glucocorticoid ablates innate toxicity of systemically delivered adenoviral vectors without reducing efficacy

Autor: Matthew Bujold, Daniel M. Appledorn, Yasser A. Aldhamen, William Nance, Sarah Godbehere, Nathaniel J. Schuldt, Tyler Voss, Andrea Amalfitano, Sergey S. Seregin, Aaron J. Mcbride, Junping Wei

Publikováno v: Molecular therapy : the journal of the American Society of Gene Therapy. 17(4)

More than 300 human clinical trials utilize recombinant adenoviruses (rAds) as a gene transfer vector, confirming that rAds continue to be of high clinical interest. A primary weakness of rAds is their known propensity to trigger an innate, proinflam

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0b4d48908d8a6328981e4483a0552e9f
https://pubmed.ncbi.nlm.nih.gov/19174760

Adenovirus vector-induced innate inflammatory mediators, MAPK signaling, as well as adaptive immune responses are dependent upon both TLR2 and TLR9 in vivo

Autor: Narayanan Parameswaran, Sonika Patial, Nico van Rooijen, Daniel M. Appledorn, Sarah Godbehere, Aaron J. Mcbride, Andrea Amalfitano

Publikováno v: Journal of Immunology, 181(3), 2134-2144. American Association of Immunologists
Appledorn, D M, Patial, S, McBride, A, Godbehere, S, van Rooijen, N, Parameswaran, N & Amalfitano, A 2008, ' Adenovirus vector-induced innate inflammatory mediators, MAPK signaling, as well as adaptive immune responses are dependent upon both TLR2 and TLR9 in vivo ', Journal of Immunology, vol. 181, no. 3, pp. 2134-2144 . https://doi.org/10.4049/jimmunol.181.3.2134

Adenovirus (Ad) vectors are promising candidates for both gene transfer and vaccine applications. In this study, we investigated the role of TLR2 in innate and adaptive immune responses to Ad and/or the transgene it expresses following systemic injec

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::00d62902c659ae5f05b7a80ff6945c66
https://research.vumc.nl/en/publications/7af665a8-1008-4d26-8265-35d92d584795