Validation of Reference Genes for Gene Expression Studies by RT-qPCR in HepaRG Cells during Toxicity Testing and Disease Modelling

Autor: Joanna Brzeszczyńska, Filip Brzeszczyński, Kay Samuel, Katie Morgan, Steven D. Morley, John N. Plevris, Peter C. Hayes

Publikováno v: Cells, Vol 9, Iss 3, p 770 (2020)

Gene expression analysis by quantitative real-time polymerase chain reaction (RT-qPCR) is routinely used in biomedical studies. The reproducibility and reliability of the data fundamentally depends on experimental design and data interpretation. Desp

Externí odkaz: https://doaj.org/article/6561e562b8a14fc7afe63fc243209f5c

The modulation of transcriptional expression and inhibition of multidrug resistance associated protein 4 (MRP4) by analgesics and their primary metabolites

Autor: Carolina Inés Ghanem, José E. Manautou, Renato J. Scialis

Publikováno v: Current Research in Toxicology, Vol 1, Iss, Pp 34-41 (2020)
CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
Current Research in Toxicology

During the course of a toxic challenge, changes in gene expression can manifest such as induction of metabolizing enzymes as a compensatory detoxification response. We currently report that a single 400 mg/kg acetaminophen (APAP) dose to C57BL/6J mic

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3df814e4c222da8ae59787676297f1bb
http://www.sciencedirect.com/science/article/pii/S2666027X20300050

Recommendations for the use of the acetaminophen hepatotoxicity model for mechanistic studies and how to avoid common pitfalls

Autor: Wen-Xing Ding, Hartmut Jaeschke, David S Umbaugh, Olamide B. Adelusi, Giselle Sanchez-Guerrero, Nga T. Nguyen, Jephte Y. Akakpo, Anup Ramachandran

Publikováno v: Acta Pharmaceutica Sinica. B
Acta Pharmaceutica Sinica B, Vol 11, Iss 12, Pp 3740-3755 (2021)

Acetaminophen (APAP) is a widely used analgesic and antipyretic drug, which is safe at therapeutic doses but can cause severe liver injury and even liver failure after overdoses. The mouse model of APAP hepatotoxicity recapitulates closely the human

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bde30b76b24a28a9236a78a6ec2b0a30
https://pubmed.ncbi.nlm.nih.gov/35024303

There Is Something Fishy About Liver Cancer: Zebrafish Models of Hepatocellular CarcinomaSummary

Autor: Isaac M. Oderberg, Paul J. Wrighton, Wolfram Goessling

Publikováno v: Cellular and Molecular Gastroenterology and Hepatology, Vol 8, Iss 3, Pp 347-363 (2019)
Cellular and Molecular Gastroenterology and Hepatology

The incidence of hepatocellular carcinoma (HCC) and the mortality resulting from HCC are both increasing. Most patients with HCC are diagnosed at advanced stages when curative treatments are impossible. Current drug therapy extends mean overall survi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::476acdf5f09ee572aaef150e6ad48289
http://www.sciencedirect.com/science/article/pii/S2352345X19300633

Intestinal Dysbiosis Amplifies Acetaminophen-Induced Acute Liver Injury

Autor: Jan G. Hengstler, Eric J. C. Gálvez, Kai Markus Schneider, Lena Susanna Candels, Till Strowig, Christoph A. Thaiss, Michael Spiteller, Ina Bergheim, Eicke Latz, Benjamin Lelouvier, Ahmed Ghallab, Lijun Liao, Maiju Myllys, Anika Nier, Carolin V. Schneider, Antje Mohs, C Elfers, Sebastian Zuehlke, Alain Roulet, Konrad Kilic, Christian Trautwein, Amirouche Ouzerdine, Wenfang Gui, Theresa H. Wirtz

Publikováno v: Cellular and Molecular Gastroenterology and Hepatology, Vol 11, Iss 4, Pp 909-933 (2021)
Cellular and Molecular Gastroenterology and Hepatology, 11(4):909-933
Cellular and Molecular Gastroenterology and Hepatology : CMGH 11(4), 909-933 (2021). doi:10.1016/j.jcmgh.2020.11.002
Cellular and Molecular Gastroenterology and Hepatology
Cellular and molecular gastroenterology and hepatology
United Kingdom
United States

Background & Aims Acute liver failure (ALF) represents an unmet medical need in Western countries. Although the link between intestinal dysbiosis and chronic liver disease is well-established, there is little evidence for a functional role of gut-liv

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::65c846d93f45d023f4eb14b075b03462
https://doi.org/10.1016/j.jcmgh.2020.11.002