Advancements in mammalian display technology for therapeutic antibody development and beyond: current landscape, challenges, and future prospects

Autor: Peter Slavny, Manjunath Hegde, Achim Doerner, Kothai Parthiban, John McCafferty, Stefan Zielonka, Rene Hoet

Publikováno v: Frontiers in Immunology, Vol 15 (2024)

The evolving development landscape of biotherapeutics and their growing complexity from simple antibodies into bi- and multi-specific molecules necessitates sophisticated discovery and engineering platforms. This review focuses on mammalian display t

Externí odkaz: https://doaj.org/article/5cdfdebb503e41e486ff999878723167

Phage display as a tool for identifying HIV-1 broadly neutralizing antibodies

Autor: A. N. Chikaev, A. P. Rudometov, Yu. A. Merkulyeva, L. I. Karpenko

Publikováno v: Вавиловский журнал генетики и селекции, Vol 25, Iss 5, Pp 562-572 (2021)

Combinatorial biology methods offer a good solution for targeting interactions of specific molecules by a high-throughput screening and are widely used for drug development, diagnostics, identification of novel monoclonal antibodies, search for linea

Externí odkaz: https://doaj.org/article/b7f9df386b8b4c8883bb4f63c84acdb9

Eliminating OFF-frame clones in randomized gene libraries: An improved split β-lactamase enrichment system

Autor: Oscar Mejias-Gomez, Andreas V. Madsen, Lasse E. Pedersen, Peter Kristensen, Steffen Goletz

Publikováno v: Mejias-Gomez, O, Madsen, A V, Pedersen, L E, Kristensen, P & Goletz, S 2023, ' Eliminating OFF-Frame Clones in Randomized Gene Libraries: An Improved Split β-Lactamase Enrichment System ', New Biotechnology, vol. 75, pp. 13-20 . https://doi.org/10.1016/j.nbt.2023.03.002
Mejias-Gomez, O, Madsen, A V, Pedersen, L E, Kristensen, P & Goletz, S 2023, ' Eliminating OFF-Frame Clones in Randomized Gene Libraries : An Improved Split β-Lactamase Enrichment System ', New Biotechnology, vol. 75, pp. 13-20 . https://doi.org/10.1016/j.nbt.2023.03.002

Large, randomized libraries are a key technology for many biotechnological applications. While genetic diversity is the main parameter most libraries direct their resources on, less focus is devoted to ensuring functional IN-frame expression. This st

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::18780147ed78ac761d066ce49eaa31cc
https://doi.org/10.1016/j.nbt.2023.03.002

Phage Display Derived Monoclonal Antibodies: From Bench to Bedside

Autor: Mohamed A. Alfaleh, Hashem O. Alsaab, Ahmad Bakur Mahmoud, Almohanad A. Alkayyal, Martina L. Jones, Stephen M. Mahler, Anwar M. Hashem

Publikováno v: Frontiers in Immunology, Vol 11 (2020)

Monoclonal antibodies (mAbs) have become one of the most important classes of biopharmaceutical products, and they continue to dominate the universe of biopharmaceutical markets in terms of approval and sales. They are the most profitable single prod

Externí odkaz: https://doaj.org/article/eb81d7b6c16242adb96c2b5f6566c87e

Efficient Construction of a Large Nonimmune Phage Antibody Library: The Production of High-Affinity Human Single-Chain Antibodies to Protein Antigens

Autor: Sheets, Michael D., Amersdorfer, Peter, Finnern, Ricarda, Sargent, Peter, Lindqvist, Ericka, Schier, Robert, Hemingsen, Grete, Wong, Cindy, Gerhart, John C., Marks, James D.

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America, 1998 May . 95(11), 6157-6162.

Externí odkaz: https://www.jstor.org/stable/45348