DEVELOPMENT OF A BIOREACTOR PROCESS FOR RECOMBINANT CELL LINES

Autor: AJ Racher, Griffiths. Jb

BHK21 cells expressing a recombinant antibody were grown in a fixed bed bioreactor (FBR) containing Siran beads. The contribution of five process variables (bead and inoculum sizes; circulation and dilution rates; feed glutamine concentration) to pro

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::aca99bcb4ee74ea529d42a15b6112ee7
https://doi.org/10.1016/b978-0-7506-1845-8.50060-0

Directed evolution of biomass intensive CHO cells by adaptation to sub-physiological temperature.

Autor: Syddall KL; Department of Chemical and Biological Engineering, University of Sheffield, Mappin St., Sheffield, S1 3JD, UK., Fernandez-Martell A; Department of Chemical and Biological Engineering, University of Sheffield, Mappin St., Sheffield, S1 3JD, UK., Cartwright JF; Department of Chemical and Biological Engineering, University of Sheffield, Mappin St., Sheffield, S1 3JD, UK., Alexandru-Crivac CN; Department of Chemical and Biological Engineering, University of Sheffield, Mappin St., Sheffield, S1 3JD, UK., Hodgson A; Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN, UK., Racher AJ; Lonza Biologics Plc, Slough, SL1 4DX, UK., Young RJ; Lonza Biologics plc, Cambridge, CB21 6GS, UK., James DC; Department of Chemical and Biological Engineering, University of Sheffield, Mappin St., Sheffield, S1 3JD, UK. Electronic address: d.c.james@sheffield.ac.uk.

Publikováno v: Metabolic engineering [Metab Eng] 2024 Jan; Vol. 81, pp. 53-69. Date of Electronic Publication: 2023 Nov 23.

Data for engineering lipid metabolism of Chinese hamster ovary (CHO) cells for enhanced recombinant protein production.

Autor: Budge JD; Industrial Biotechnology Centre, School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, UK., Knight TJ; Industrial Biotechnology Centre, School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, UK., Povey J; Industrial Biotechnology Centre, School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, UK., Roobol J; Industrial Biotechnology Centre, School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, UK., Brown IR; Industrial Biotechnology Centre, School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, UK., Singh G; Industrial Biotechnology Centre, School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, UK., Dean A; Lonza Biologics, 228 Bath Road, Slough, SL1 4DX, UK., Turner S; Lonza Biologics, 228 Bath Road, Slough, SL1 4DX, UK., Jaques CM; Lonza Biologics, 228 Bath Road, Slough, SL1 4DX, UK., Young RJ; Cell Engineering Group, Lonza Biologics, Granta Park, Cambridge, CB21 6GS, UK., Racher AJ; Lonza Biologics, 228 Bath Road, Slough, SL1 4DX, UK., Smales CM; Industrial Biotechnology Centre, School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, UK.

Publikováno v: Data in brief [Data Brief] 2020 Jan 31; Vol. 29, pp. 105217. Date of Electronic Publication: 2020 Jan 31 (Print Publication: 2020).

Intact-Cell MALDI-ToF Mass Spectrometry for the Authentication of Drug-Adapted Cancer Cell Lines.

Autor: Povey JF; Industry Biotechnology Centre and School of Biosciences, University of Kent, Canterbury CT2 7NJ, UK. J.Povey@kent.ac.uk., Saintas E; Industry Biotechnology Centre and School of Biosciences, University of Kent, Canterbury CT2 7NJ, UK. esaintas@icloud.com., Aderemi AV; Industry Biotechnology Centre and School of Biosciences, University of Kent, Canterbury CT2 7NJ, UK. walerem@yahoo.com., Rothweiler F; Institut für Medizinische Virologie, Klinikum der Goethe-Universität, 60596 Frankfurt am Main, Germany. f.rothweiler@kinderkrebsstiftung-frankfurt.de., Zehner R; Institut für Rechtsmedizin, Klinikum der Goethe-Universität, 60596 Frankfurt am Main, Germany. zehner@em.uni-frankfurt.de., Dirks WG; Leibniz-Institute Deutsche Sammlung für Mikroorganismen und Zellkulturen GmbH, 38124 Braunschweig, Germany. wdi@dsmz.de., Cinatl J Jr; Institut für Medizinische Virologie, Klinikum der Goethe-Universität, 60596 Frankfurt am Main, Germany., Racher AJ; Lonza Biologics, Slough SL1 4DX, UK. andy.racher@lonza.com., Wass MN; Industry Biotechnology Centre and School of Biosciences, University of Kent, Canterbury CT2 7NJ, UK. m.n.wass@kent.ac.uk., Smales CM; Industry Biotechnology Centre and School of Biosciences, University of Kent, Canterbury CT2 7NJ, UK. C.M.Smales@kent.ac.uk., Michaelis M; Industry Biotechnology Centre and School of Biosciences, University of Kent, Canterbury CT2 7NJ, UK. M.Michaelis@kent.ac.uk.

Publikováno v: Cells [Cells] 2019 Oct 02; Vol. 8 (10). Date of Electronic Publication: 2019 Oct 02.

Implementation of Plate Imaging for Demonstration of Monoclonality in Biologics Manufacturing Development.

Autor: Zingaro K; Alexion Pharmaceuticals, Inc., New Haven, CT, USA; kyle.zingaro@gmail.com david.shaw@gene.com., Shaw D; Genentech, Inc., South San Francisco, CA, USA; kyle.zingaro@gmail.com david.shaw@gene.com., Carson J; AbbVie Bioresearch Center, Worcester, MA, USA., Mayer-Bartschmid A; Bayer AG, Pharmaceuticals, Research and Development, Cell and Protein Sciences, Wuppertal, Germany., Bender C; Bayer AG, Pharmaceuticals, Research and Development, Cell and Protein Sciences, Wuppertal, Germany., Alves C; Biogen, Cell Culture Development, Cambridge, MA, USA., Mcvey D; Bristol-Myers Squibb, Pennington, NJ, USA., Qian NX; Bristol-Myers Squibb, Devens, MA, USA., Wei Q; Celgene Corporation, Summit West, Summit, NJ, USA., Laird MW; Genentech, Inc., South San Francisco, CA, USA., Zhu Y; GSK, King of Prussia, PA, USA., Emmins R; GSK, Stevenage, UK., Follit JA; ImmunoGen, Inc., Waltham, USA., Porter A; Lonza Biologics plc, Slough, UK., Racher AJ; Lonza Biologics plc, Slough, UK., Milne S; Lonza Biologics plc, Slough, UK., Carubelli I; Lonza Biologics plc, Slough, UK., Du Z; Merck & Co., Inc., Kenilworth, NJ, USA., Khatri A; Merck & Co., Inc., Kenilworth, NJ, USA., Failly M; Merck Biodevelopment, Martillac, France., Broly H; Merck Serono S.A., Fenil-sur-Corsier, Switzerland., Lee F; Morphotek, Inc., Exton, PA, USA., Reeser M; Morphotek, Inc., Exton, PA, USA., Spidel J; Morphotek, Inc., Exton, PA, USA., Anderson K; Pfizer BioTherapeutics Pharmaceutical Sciences, Andover, MA, USA., Demaria C; Sanofi Biopharmaceutics Development, Framingham, MA USA., Di-Carlo J; Sanofi Biopharmaceutics Development, Vitry-sur-Seine, France., Gill J; Shire, Lexington, MA, USA., Lundquist A; Shire, Lexington, MA, USA., Kumar SR; Takeda Pharmaceuticals International Co., Cambridge, MA, USA; and., Gill T; BioPhorum Development Group, Sheffield, UK.

Publikováno v: PDA journal of pharmaceutical science and technology [PDA J Pharm Sci Technol] 2018 Jul-Aug; Vol. 72 (4), pp. 438-450. Date of Electronic Publication: 2018 Apr 18.