Monitoring ER Ca ²⁺ by Luminescence with Low Affinity GFP-Aequorin Protein (GAP).

Autor: Rodriguez-Prados M; Instituto de Biomedicina y Genética Molecular de Valladolid (IBGM), Unidad de Excelencia, Universidad de Valladolid y Consejo Superior de Investigaciones Científicas (CSIC), Valladolid, Spain.; Universidad Alfonso X el Sabio, Madrid, Spain., Rojo-Ruiz J; Instituto de Biomedicina y Genética Molecular de Valladolid (IBGM), Unidad de Excelencia, Universidad de Valladolid y Consejo Superior de Investigaciones Científicas (CSIC), Valladolid, Spain., Calvo B; Instituto de Biomedicina y Genética Molecular de Valladolid (IBGM), Unidad de Excelencia, Universidad de Valladolid y Consejo Superior de Investigaciones Científicas (CSIC), Valladolid, Spain., Garcia-Sancho J; Instituto de Biomedicina y Genética Molecular de Valladolid (IBGM), Unidad de Excelencia, Universidad de Valladolid y Consejo Superior de Investigaciones Científicas (CSIC), Valladolid, Spain., Alonso MT; Instituto de Biomedicina y Genética Molecular de Valladolid (IBGM), Unidad de Excelencia, Universidad de Valladolid y Consejo Superior de Investigaciones Científicas (CSIC), Valladolid, Spain. talonso@uva.es.

Publikováno v: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2025; Vol. 2861, pp. 141-153.

Detection of dopamine levels using a polydiacetylene liposomal aequorin bioluminescent device with octadecylboronic acid.

Autor: Takegami S; Laboratory of Analytical Chemistry, Kyoto Pharmaceutical University, 5 Misasaginakauchi-cho, Yamashina-ku, Kyoto, 607-8414, Japan. takegami@mb.kyoto-phu.ac.jp., Danzako M; Laboratory of Analytical Chemistry, Kyoto Pharmaceutical University, 5 Misasaginakauchi-cho, Yamashina-ku, Kyoto, 607-8414, Japan., Konishi A; Laboratory of Analytical Chemistry, Kyoto Pharmaceutical University, 5 Misasaginakauchi-cho, Yamashina-ku, Kyoto, 607-8414, Japan.

Publikováno v: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry [Anal Sci] 2024 Feb; Vol. 40 (2), pp. 353-356. Date of Electronic Publication: 2023 Dec 08.

Analysis of abiotic and biotic stress-induced Ca ²⁺ transients in the crop species Solanum tuberosum.

Autor: van Dieren A; Institute for Cellular and Molecular Botany, University of Bonn, Kirschallee 1, 53115, Bonn, Germany. adieren@uni-bonn.de., Schwarzenbacher RE; Department of Biosciences, Durham University, South Road, Durham, DH1 3LE, UK., Sonnewald S; Department of Biology, Chair of Biochemistry, Friedrich-Alexander-University Erlangen-Nuremberg, Staudtstraße 5, Erlangen, 91058, Germany., Bittner A; Institute for Cellular and Molecular Botany, University of Bonn, Kirschallee 1, 53115, Bonn, Germany., Vothknecht UC; Institute for Cellular and Molecular Botany, University of Bonn, Kirschallee 1, 53115, Bonn, Germany. vothknecht@uni-bonn.de.

Publikováno v: Scientific reports [Sci Rep] 2024 Nov 11; Vol. 14 (1), pp. 27625. Date of Electronic Publication: 2024 Nov 11.

Bioluminescent immunoassay for serine/threonine protein kinase activity using an aequorin-labeled monoclonal antibody and a synthetic peptide as a substrate.

Autor: Sato JI; Yokohama Research Center, JNC Co., 5-1 Okawa, Kanazawa-ku, Yokohama, 236-8605, Japan., Onogi H; KinoPharma, Inc. Kyoto-University Katsura Venture Plaza 1-39 Goryoohara, Nishikyo-ku, Kyoto, 615-8245, Japan. Electronic address: honogi@kinopharma.com., Nomura N; KinoPharma, Inc. Kyoto-University Katsura Venture Plaza 1-39 Goryoohara, Nishikyo-ku, Kyoto, 615-8245, Japan., Hagiwara M; Department of Anatomy and Developmental Biology, Kyoto University Graduate School of Medicine, Sakyo-Ku, Kyoto, 606-8501, Japan., Inouye S; Yokohama Research Center, JNC Co., 5-1 Okawa, Kanazawa-ku, Yokohama, 236-8605, Japan. Electronic address: sinouye@dpc.agu.ac.jp.

Publikováno v: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2023 Nov 12; Vol. 681, pp. 180-185. Date of Electronic Publication: 2023 Sep 22.

The Role of Tyr-His-Trp Triad and Water Molecule Near the N1-Atom of 2-Hydroperoxycoelenterazine in Bioluminescence of Hydromedusan Photoproteins: Structural and Mutagenesis Study.

Autor: Natashin PV; Photobiology Laboratory, Institute of Biophysics of Siberian Branch of the Russian Academy of Sciences, Federal Research Center 'Krasnoyarsk Science Center' of Siberian Branch of the Russian Academy of Sciences', Krasnoyarsk 660036, Russia., Burakova LP; Photobiology Laboratory, Institute of Biophysics of Siberian Branch of the Russian Academy of Sciences, Federal Research Center 'Krasnoyarsk Science Center' of Siberian Branch of the Russian Academy of Sciences', Krasnoyarsk 660036, Russia.; Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Krasnoyarsk 660041, Russia., Kovaleva MI; Research Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny 141701, Russia., Shevtsov MB; Research Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny 141701, Russia., Dmitrieva DA; Research Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny 141701, Russia., Eremeeva EV; Photobiology Laboratory, Institute of Biophysics of Siberian Branch of the Russian Academy of Sciences, Federal Research Center 'Krasnoyarsk Science Center' of Siberian Branch of the Russian Academy of Sciences', Krasnoyarsk 660036, Russia.; Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Krasnoyarsk 660041, Russia., Markova SV; Photobiology Laboratory, Institute of Biophysics of Siberian Branch of the Russian Academy of Sciences, Federal Research Center 'Krasnoyarsk Science Center' of Siberian Branch of the Russian Academy of Sciences', Krasnoyarsk 660036, Russia.; Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Krasnoyarsk 660041, Russia., Mishin AV; Research Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny 141701, Russia., Borshchevskiy VI; Research Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny 141701, Russia.; Joint Institute for Nuclear Research, Dubna 141980, Russia., Vysotski ES; Photobiology Laboratory, Institute of Biophysics of Siberian Branch of the Russian Academy of Sciences, Federal Research Center 'Krasnoyarsk Science Center' of Siberian Branch of the Russian Academy of Sciences', Krasnoyarsk 660036, Russia.

Publikováno v: International journal of molecular sciences [Int J Mol Sci] 2023 Apr 06; Vol. 24 (7). Date of Electronic Publication: 2023 Apr 06.