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Akademický článek

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Reconstruction of ooplasm recipient oocytes with frozen-thawed donor microcytoplasts and influence on the microtubular spindle

Autor: Pravin T. Goud, Mark R. Hughes, Michael P. Diamond, Bernard Gonik, A.P. Goud, D. Dozortsev

Publikováno v: Fertility and Sterility. 87:923-933

Objective(s) To cryopreserve micromanipulated ooplast segments (microcytoplasts) from mouse oocytes, compare microcytoplast and parent or recipient oocyte fusion performed within or without the zona pellucida, compare electrofusion of fresh or frozen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7012936095b5efc1ed67b301a224b9df
https://doi.org/10.1016/j.fertnstert.2006.07.1529

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Microtubule turnover in ooplasm biopsy reflects ageing phenomena in the parent oocyte

Autor: P. Van Oostveldt, A.P. Goud, Hughes, Pravin T. Goud, Michael P. Diamond

Publikováno v: Reproductive BioMedicine Online. 11:43-52

Oviductal oocytes retrieved from superovulated B6D2F1 mice at 13.5, 16 and 19 h after human chorionic gonadotrophin (HCG) (groups A, B and C respectively, n = 382) were micromanipulated to obtain 12-20 mum sized ooplasm biopsy fragments. Experiments

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3ade0f4b8f18c5ffd447a62559feea14
https://doi.org/10.1016/s1472-6483(10)61297-7

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Inositol 1,4,5-trisphosphate receptor function in human oocytes: calcium responses and oocyte activation-related phenomena induced by photolytic release of InsP3 are blocked by a specific antibody to the type I receptor

Autor: Marc Dhont, A.P. Goud, P. Van Oostveldt, Michael P. Diamond, K. Mikoshiba, Pravin T. Goud, Luc Leybaert

Publikováno v: Molecular Human Reproduction. 8:912-918

Type I inositol 1,4,5-trisphosphate-sensitive receptors (InsP(3)R) are expressed in human oocytes and may be involved in operating the Ca(2+) release triggered by the fertilizing sperm. This study examines the contribution of type I InsP(3)R in opera

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::da5229bcf5a731c18b5661ebee0bb45b
https://doi.org/10.1093/molehr/8.10.912

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Direct real-time measurement of intra-oocyte nitric oxide concentration in vivo

Autor: A.P. Goud, Bernard Gonik, Husam M. Abu-Soud, Pravin T. Goud, Ghassan M. Saed, Michael P. Diamond, Tohid Najafi, Xueji Zhang

Publikováno v: PLoS ONE, Vol 9, Iss 6, p e98720 (2014)
PLoS ONE

Nitric oxide (NO) is reported to play significant a role in oocyte activation and maturation, implantation, and early embryonic development. Previously we have shown that NO forms an important component of the oocyte microenvironment, and functions e

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3c9886a5d57a7442c684bdf327c89397
http://europepmc.org/articles/PMC4041775?pdf=render

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Presence and dynamic redistribution of type I inositol 1,4,5-trisphosphate receptors in human oocytes and embryos during in-vitro maturation, fertilization and early cleavage divisions

Autor: Marc Dhont, P. Van Oostveldt, Pravin T. Goud, A.P. Goud

Publikováno v: Molecular Human Reproduction. 5:441-451

We studied the presence and distribution of the intracellular calcium channel regulating type I inositol 1,4,5-trisphosphate receptors (IP3R) in human immature and mature oocytes, pronuclear zygotes and cleaved embryos using a specific antibody. Two

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::821a190c913723ee19de235989b3298b
https://doi.org/10.1093/molehr/5.5.441

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Effect of post-ovulatory age and calcium in the injection medium on the male pronucleus formation and metaphase entry following injection of human spermatozoa into golden hamster oocytes

Autor: A.P. Goud, H Laverge, P. De Sutter, Pravin T. Goud, Marc Dhont

Publikováno v: Molecular Human Reproduction. 5:227-233

The occurrence of parthenogenetic activation is a major hurdle in obtaining sperm chromosome metaphases after heterospecific intracytoplasmic sperm injection (ICSI) of golden hamster oocytes with human spermatozoa. We addressed two potential contribu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f192b056124ae770c245947f87cdf1d3
https://doi.org/10.1093/molehr/5.3.227

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Chromatin decondensation, pronucleus formation, metaphase entry and chromosome complements of human spermatozoa after intracytoplasmic sperm injection into hamster oocytes

Autor: Marc Dhont, Andrei Rybouchkin, P.T. Goud, P. De Sutter, A.P. Goud

Publikováno v: Human Reproduction. 13:1336-1345

Obtaining karyotypes from human spermatozoa after microinjection into Syrian golden hamster oocytes is difficult and the hitherto reported results are unsatisfactory. This may be related to the injection and culture technique or to the high susceptib

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b4b6d2cfd6e7f203960296c018ad4f65
https://doi.org/10.1093/humrep/13.5.1336

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Dynamics of nitric oxide, altered follicular microenvironment, and oocyte quality in women with endometriosis

Autor: A.P. Goud, Husam M. Abu-Soud, Narendra J. Joshi, Michael P. Diamond, Elizabeth E. Puscheck, Pravin T. Goud

Publikováno v: Fertility and sterility. 102(1)

Objective To study follicular microenvironment in terms of free radical dynamics, oocyte quality, and assisted reproductive technology (ART) outcomes among women with (group A) and without (group B) endometriosis. Design Prospective cohort study. Set

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a631053a49cc34fa685f91a146b2003e
https://pubmed.ncbi.nlm.nih.gov/24825428

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