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pro vyhledávání: '"A.M. Ashby"'
Publikováno v:
Physiological and Molecular Plant Pathology. 62:13-20
An extracellular serine protease, designated Pyrenopeziza brassicae serine protease 1 (Psp1), was purified by a combination of ammonium sulphate precipitation and fast protein liquid chromatography (FPLC) to yield a single 34 kDa band on SDS–PAGE w
Publikováno v:
European Journal of Plant Pathology. 108:379-383
An existing PCR-based method for diagnosis of the winter oilseed rape (Brassica napus ssp oleifera) fungal pathogen Pyrenopeziza brassicae (cause of light leaf spot) was improved by the development of a pair of primers (PbN1 and PbN2) for use in nest
Publikováno v:
Mycological Research. 105:705-714
The development of apothecia of Pyrenopeziza brassicae (anamorph Cylindrosporium concentricum) on oilseed rape debris and compost malt agar was observed by scanning electron and light microscopy. On oilseed rape debris, apothecia developed directly b
Autor:
A.M. Ashby
Publikováno v:
Physiological and Molecular Plant Pathology. 57:147-158
Cytokinin biosynthesis in plants has been studied for well over 40 years, yet despite intensive research we are still no closer to understanding the molecular steps involved in this pathway. In addition to well established effects in plants, it is cl
Publikováno v:
Physiological and Molecular Plant Pathology. 57:63-75
Supernatants from apple cutin-induced cultures of the light leaf spot pathogen Pyrenopeziza brassicae were shown to contain a single esterase with a pI of approx. 4.4 and a MW of 21 kDa. Analysis of the hydrolysis products derived from enzymatic clea
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1497:168-174
A septin gene homologue designated Pyrenopeziza brassicae septin 1 (Pbs1) has been identified and cloned from the plant pathogenic fungus Pyrenopeziza brassicae and its expression analysed. Pbs1 is present in both mating types and in a single copy wi
Publikováno v:
Physiological and Molecular Plant Pathology. 55:111-119
Two sets of PCR primers were designed from DNA sequence which flanked the 3′ end of the Pyrenopeziza brassicae mating type idiomorphs (MAT-1 and MAT-2). The first set of primers facilitated the amplification of a 750 bp product from genomic DNA of
Autor:
A.M. Ashby, Gurjeet Singh
Publikováno v:
Molecular Microbiology. 30:799-806
The mating type loci were cloned from Pyrenopeziza brassicae by chromosome walking from a mating type-linked polymerase chain reaction (PCR) fragment and shown to be idiomorphic by sequence analysis. The MAT 1-1 locus is approximately 3.2 kb and cont
Autor:
A.M. Ashby
Publikováno v:
Physiological and Molecular Plant Pathology. 53:1-15
Protein profiles from extracts of single mating type isolates ofPyrenopeziza brassicaeinduced by a lipoidal sex factor (SF) from mated cultures, and of individual picked mature fertile apothecia from a sexual cross were analysed. A major protein in S
Autor:
A.M. Ashby, S.J Cooper
Publikováno v:
Physiological and Molecular Plant Pathology. 53:61-72
Early symptoms of apple scab caused by Venturia inaequalis include leaf curl and green island formation, both of which are indicative of an imbalance of plant growth regulators. To test whether V. inaequalis has the potential to contribute to this ph