Výsledky vyhledávání - "A.M Noubhani"

Purification and on-column refolding of EGFP overexpressed as inclusion bodies in Escherichia coli with expanded bed anion exchange chromatography

Autor: Xavier Santarelli, F. Dole, A.M Noubhani, Charlotte Cabanne, A. Hocquellet, Wilfrid Dieryck

Publikováno v: Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, Elsevier, 2005, 818, pp. 23-27. ⟨10.1016/j.jchromb.2004.10.023⟩

5 pages; International audience; The enhanced green fluorescent protein (EGFP) was over-expressed in Escherichia coli as inclusion bodies to increase its quantity and to facilitate its purification. Insoluble EGFP has been purified on Q Hyper Z matri

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0feae45b936e80ce13a2cc562ac6d915
https://doi.org/10.1016/j.jchromb.2004.10.023

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Penicillin acylase purification with the aid of hydrophobic charge induction chromatography

Autor: V. Fitton, E Saint-Christophe, D Coulon, A.M Noubhani, Charlotte Cabanne, Xavier Santarelli

Publikováno v: Journal of Chromatography B. 808:111-115

The aim of this work was to test a chromatographic support, 4-mercaptoethyl pyridine (4-MEP) Hypercel, for penicillin acylase purification by using pure penicillin acylase and crude extract. Two equilibration buffers with various salt concentrations

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2e2c0e7270236a5cface995fa763f058
https://doi.org/10.1016/j.jchromb.2004.03.025

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Evaluation of three expanded bed adsorption anion exchange matrices with the aid of recombinant enhanced green fluorescent protein overexpressed in Escherichia coli

Autor: Wilfrid Dieryck, Charlotte Cabanne, A. Hocquellet, A.M Noubhani, Xavier Santarelli

Publikováno v: Journal of Chromatography B. 808:91-97

Three anion exchanger expanded bed adsorption (EBA) matrices: Streamline DEAE, Streamline Q XL and Q Hyper Z were evaluated with the aid of EFGP from an ultrasonic homogenate of Escherichia coli. Two pH of buffer were tested. Capture was done in an e

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::32a93fe495cc1c803829afeb0fff3104
https://doi.org/10.1016/j.jchromb.2004.02.009

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Cloning, expression and two-step purification of recombinant His-tag enhanced green fluorescent protein over-expressed in Escherichia coli

Autor: Wilfrid Dieryck, D Coulon, A.M Noubhani, Xavier Santarelli

Publikováno v: Journal of Chromatography B. 786:153-159

In this report, we describe a two-step chromatographic procedure for the purification of His-tag EGFP by immobilized metal affinity expanded bed adsorption (IMAEBA) as the capture step and size exclusion chromatography as the polishing step. The use

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::81078281ccf6f4a112a7be105c6a56da
https://doi.org/10.1016/s1570-0232(02)00764-x

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On-line purification of His-tag enhanced green fluorescent protein taken directly from a bioreactor by continuous ultrasonic homogenization coupled with immobilized metal affinity expanded bed adsorption

Autor: S Chevalier, Xavier Santarelli, Wilfrid Dieryck, A.M Noubhani

Publikováno v: Journal of Chromatography A. 968:113-120

In this report, we describe a new process for the on-line purification of His-tag EGFP (enhanced green fluorescent protein) taken directly from a bioreactor by continuous ultrasonic homogenization coupled with immobilized metal affinity expanded bed

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::09d88726ad165b8f92518af9726b8da4
https://doi.org/10.1016/s0021-9673(02)00794-x

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Evaluation of chromatographic recycling for imidazole used in the chromatographic purification of His-tag recombinant proteins

Autor: Xavier Santarelli, Wilfrid Dieryck, A.M Noubhani, Laurent Latxague, Norbert Bakalara

Publikováno v: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 790(1-2)

The aim of this work was to test a recycling method for imidazole used in immobilized metal affinity chromatography (IMAC) as eluent for recombinant histidine-tag (His-tag) protein. After evaluating two supports, the method was optimized with a mixtu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c983a0886041e424f0c7bd566f732d31
https://pubmed.ncbi.nlm.nih.gov/12767328

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