Zobrazeno 1 - 10
of 12
pro vyhledávání: '"A.Keith Stobart"'
Autor:
Frédéric Beaudoin, A.Keith Stobart, Baoxiu Qi, T. Fraser, Johnathan A. Napier, Colin M. Lazarus
Publikováno v:
FEBS Letters. 510:159-165
Isochrysis galbana, a marine prymnesiophyte microalga, is rich in long chain polyunsaturated fatty acids such as docosahexaenoic acid (C22:6n-3, Δ4,7,10,13,16,19). We used a polymerase chain reaction-based strategy to isolate a cDNA, designated IgAS
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5423ce23dc5ba9affd854940bf786526
https://doi.org/10.1016/s0014-5793(01)03247-1
https://doi.org/10.1016/s0014-5793(01)03247-1
Autor:
Colin M. Lazarus, Louise V. Michaelson, Johnathan A. Napier, Mervyn J. Lewis, Gareth Griffiths, A.Keith Stobart
Publikováno v:
FEBS Letters. 439:215-218
We have identified a cDNA from the nematode worm Caenorhabditis elegans that encodes a fatty acid delta5 desaturase. Saccharomyces cerevisiae expressing the full-length cDNA was able to convert di-homo-gamma-linolenic acid to arachidonic acid, thus c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::25f5a053d81b7c7e9442321b23bdbb8e
https://doi.org/10.1016/s0014-5793(98)01385-4
https://doi.org/10.1016/s0014-5793(98)01385-4
Publikováno v:
Phytotherapy Research. 12:417-421
Sesamin (a lignan present in sesame oil) is reported to specifically inhibit Δ5 desaturation and the formation of arachidonic acid (20:4n-6) from dihomogamma-linolenic acid (20:3n-6) in cell free extracts. In this study the effects of sesamin have b
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::69a4e1de88a7173177686d329a7e18c0
https://doi.org/10.1002/(sici)1099-1573(199809)12:6<417::aid-ptr334>3.0.co
https://doi.org/10.1002/(sici)1099-1573(199809)12:6<417::aid-ptr334>3.0.co
Publikováno v:
Phytochemistry. 48:261-267
Delta-9-desaturase (Δ9d) activity has been studied in the 100,000 g soluble protein fraction derived from the developing seed cotyledons of sunflower at a stage of active oil synthesis. In the presence of NADPH, ferredoxin (fd) and ferredoxin oxidor
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5ca6fd1d2b7e9d546900864b58709a07
https://doi.org/10.1016/s0031-9422(97)00855-8
https://doi.org/10.1016/s0031-9422(97)00855-8
Publikováno v:
The Prostate. 31:29-36
BACKGROUND. Polyunsaturated fatty acids (n-6) are reported to selectively kill malignant cells. Most investigations, however, did not compare neoplastic with non-neoplastic cells from the same tissue type. Here we evaluate the effects of n-6 fatty ac
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d6501eae36cc3b03a6d739df44952fa0
https://doi.org/10.1002/(sici)1097-0045(19970401)31:1<29::aid-pros5>3.0.co
https://doi.org/10.1002/(sici)1097-0045(19970401)31:1<29::aid-pros5>3.0.co
Autor:
Frances Jackson, William W. Christie, Gareth Griffiths, Elizabeth Y. Brechany, A.Keith Stobart, Sten Stymne
Publikováno v:
Phytochemistry. 43:381-386
An octadecatetraenoic acid was present as a major fatty acid component of the leaf lipids of borage. Gas chromatography-mass spectrometry of its picolinyl esters gave unsaturation centres at carbons Δ 6 , Δ 9 , Δ 12 , and Δ 15 and confirmed its i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8165d30a5cb389a96f93c4ec98376e56
https://doi.org/10.1016/0031-9422(96)00305-6
https://doi.org/10.1016/0031-9422(96)00305-6
Autor:
Baoxiu Qi, Elisabeth M. Shaw, Colin M. Lazarus, Thomas Fraser, Claire L. Bleakley, A.Keith Stobart
Publikováno v:
FEBS Letters. 547:137-139
IgASE1, a C18-Δ9-polyunsaturated fatty acid-specific fatty acid elongase component from Isochrysis galbana, contains a variant histidine box (his-box) with glutamine replacing the first histidine of the conserved histidine-rich motif present in all
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b180b889b105021d01e4d2f44e7e622a
https://doi.org/10.1016/s0014-5793(03)00676-8
https://doi.org/10.1016/s0014-5793(03)00676-8
Autor:
Baoxiu Qi, Justine Butler, Olga Sayanova, Gary Dobson, Johnathan A. Napier, A.Keith Stobart, Sam T. Mugford, T. Fraser, Colin M. Lazarus
We report the production of two very long chain polyunsaturated fatty acids, arachidonic acid (AA) and eicosapentaenoic acid (EPA), in substantial quantities in a higher plant. This was achieved using genes encoding enzymes participating in the omega
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d8751a88dd44e8b2f54ae3b149158de6
https://repository.rothamsted.ac.uk/download/dbfc77c20ffbfb17385f95964f9dbcb24e44e95da439a3141f099bef6a9dae62/3614379/nbt972.pdf
https://repository.rothamsted.ac.uk/download/dbfc77c20ffbfb17385f95964f9dbcb24e44e95da439a3141f099bef6a9dae62/3614379/nbt972.pdf
Autor:
Ruth Quayle, Johnathan A. Napier, Hifza Mazhar, A.Keith Stobart, Roger J. Fido, Peter R. Shewry
A combination of biochemical analyses and Northern blotting were used to determine the patterns of storage reserve (oil, 2S albumin, helianthinin, oleosin) synthesis during the early stages of embryo development in sunflower. This demonstrated that o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::85ed568b44164c4068441edd82253b98
Autor:
Peter R. Shewry, Arthur S. Tatham, Patrick J.E. Thoyts, Johnathan A. Napier, W. Trevor Griffiths, Mark Millichip, A.Keith Stobart
An Mr 22 500 protein was purified from isolated oil bodies from sunflower cotyledons. N-terminal amino acid sequencing showed that this protein belonged to the 2S albumin group of storage proteins. A corresponding cDNA clone encoded a preproprotein,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a0b53e647d02b677fbbdbf626712aaca