Zobrazeno 1 - 10
of 46
pro vyhledávání: '"A.J.C. Stahl"'
Publikováno v:
Research in Immunology. 143:17-23
Human low-density lipoprotein (LDL) was glycated in vitro (5 days, glucose 50 mmol/l), labelled with 125I, and its binding and uptake by human monocyte-derived macrophages studied. Glycation produced lower binding and lower uptake. Competition experi
Autor:
A.J.C. Stahl, A.Gugliucci Creriche
Publikováno v:
Journal of immunological methods. 174(1-2)
An assay for scavenger receptors on human monocyte-derived macrophages is described: the preparation of the two main ligands used in the assay is given in detail, and a protocol for 125I-acetyl-LDL binding measurements to monocyte-derived macrophages
Autor:
A. Gugliucci, A.J.C Stahl
Publikováno v:
Clinica chimica acta; international journal of clinical chemistry. 204(1-3)
Autor:
A.J.C. Stahl, M. Diatewa
Publikováno v:
Biochemical and Biophysical Research Communications. 94:189-198
Summary Yeast mitochondrial Phe-tRNA synthetase is purified about 380-fold to homogeneity by precipitation with ammonium sulfate and chromatography on DEAE-cellulose, on two hydroxylapatite and on two AH-Sepharose 4B columns. The yield is 17%. The mo
Publikováno v:
Biochemical and Biophysical Research Communications. 70:997-1002
The nucleoside composition of tRNA from highly purified yeast mitochondria shows the presence of T, ψ, hU, m1G, m2G, m22G, I and t6A whereas neither m7G, m5C, m3C, m1A, i6A and Y nor O′-methylated nucleosides (which are common in yeast cytoplasmic
Publikováno v:
Biochemical and Biophysical Research Communications. 98:727-734
Yeast mitochondrial tRNA synthetase has been partially purified and chromatographic, catalytic and antigenic properties have been compared to the cytoplasmic homologous enzyme from yeast. No significant differences could be observed between the two e
Publikováno v:
Biochemical and Biophysical Research Communications. 64:1046-1053
Reversed phase chromatography of mitochondrial [3H] Phe-tRNA from Saccharomyces cerevisiae shows only one peak which elutes distinctly from cytoplasmic [14C] Phe-tRNA. Mitochondrial tRNAPhe from this peak hybridizes specifically with ϱ+ and a ϱ−
Autor:
I. Viard, A.J.C. Stahl
Publikováno v:
FEBS Letters. 180:117-121
Proteolytic activity in yeast mitochondrial membranes and soluble fractions was studied using a labeled substrate: [ 14 C]dimethylcasein. Optimal activity for the two fractions was at pH 6 in phosphate buffer at 37°C. Activity was partially inhibite
Publikováno v:
Biochemical and Biophysical Research Communications. 106:520-525
The α and β subunits of yeast mitochondrial Phe-tRNA synthetase are separated and isolated by means of chromatography on DEAE-cellulose, after enzyme alkylation with iodoacetate. The comparison of amino acid compositions of yeast mitochondrial and
Publikováno v:
Biochimie. 63:67-69
Summary Conversion of large amounts of Saccharomyces cerevisiae cells to protoplasts is studied using various preparations extracted from Helix pomatia hepato-pancreatic juice. The most favorable yield in two hours incubations (88 per cent) is obtain