Výsledky vyhledávání - "A.J. Verkleij"

Ultrastructural co-localization of calmodulin and B-50/growth-associated protein-43 at the plasma membrane of proximal unmyelinated axon shafts studied in the model of the regenerating rat sciatic nerve

Autor: A.J. Verkleij, W.H. Gispen, Paul Verkade, A.B. Oestreicher, L.H. Schrama

Publikováno v: Neuroscience. 79:1207-1218

Calmodulin and de-phosphorylated B-50/growth-associated protein-43 (GAP-43) have been shown to bind in vitro in a molecular complex, but evidence for an in situ association in the nervous system does not exist. Previously, we have reported that, in t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0d3911ba35a252585142b218069a1cc4
https://doi.org/10.1016/s0306-4522(97)00041-9

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Ultrastructural evidence for the lack of co-transport of B-50/GAP-43 and calmodulin in myelinated axons of the regenerating rat sciatic nerve

Autor: Willem Hendrik Gispen, Paul Verkade, A.J. Verkleij, A. B. Oestreicher

Publikováno v: Journal of Neurocytology. 25:583-595

Following peripheral nerve injury, neurons respond with synthesis of proteins required for axonal regeneration. Newly synthesized membrane proteins, like B-50/GAP-43, are transported with the fast component of anterograde axonal transport. Structural

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::861b8dc39d8277645dc8b5a9adbba66f
https://doi.org/10.1007/bf02284826

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The increase in B-50/GAP-43 in regenerating rat sciatic nerve occurs predominantly in unmyelinated axon shafts: A quantitative ultrastructural study

Autor: A.J. Verkleij, A. B. Oestreicher, Paul Verkade, Willem Hendrik Gispen

Publikováno v: The Journal of comparative neurology, 356, 433. Wiley

The growth-associated protein B-50/GAP-43 is thought to play a crucial role in axonal growth. We investigated, by quantitative immunoelectron microscopy, whether there are differences in the subcellular distribution of B-50 in unmyelinated and myelin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cce8d85468c676727102efa7a4750f44
https://doi.org/10.1002/cne.903560310

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Ultrastructural localization of B-50/growth-associated protein-43 to anterogradely transported synaptophysin-positive and calcitonin gene-related peptide-negative vesicles in the regenerating rat sciatic nerve

Autor: Paul Verkade, W.G. Annaert, A.J. Verkleij, A. B. Oestreicher, W.H. Gispen

Publikováno v: Neuroscience. 71(2)

The growth-associated protein-43/B-50 (B-50/GAP-43) is conveyed from the neuronal soma into the axon by fast axonal transport and moved to the nerve terminal. To visualize and determine the type of vesicles by which B-50/GAP-43 is anterogradely trans

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4b6fd790d3c2434ea722dc182816b553
https://pubmed.ncbi.nlm.nih.gov/9053802

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Effects of various cryoprotective agents and membrane-stabilizing compounds on bull sperm membrane integrity after cooling and freezing

Autor: A.M. de Leeuw, F.E. De Leeuw, Ben Colenbrander, J.H.G. den Daas, A.J. Verkleij

Publikováno v: Cryobiology. 30(1)

In this study attempts were made to improve the survival rates of bull spermatozoa after freezing/thawing and to clarify the importance of certain agents to the cryopreservation of spermatozoa. For that purpose the standard freezing extender was modi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ea69f74d2067b9da17ac399b475d648e
https://pubmed.ncbi.nlm.nih.gov/8440128

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B-50/GAP43 localization on membranes of putative transport vesicles in the cell body, neurites and growth cones of cultured hippocampal neurons

Autor: A.J. Verkleij, A.B. Oestreicher, W.H. Gispen, C.G. Dotti, M. Van Lookeren Campagne

Publikováno v: Neuroscience letters. 137(1)

We conducted an electron microscopic immunocytochemical study to locate B-50/GAP43 in various cellular elements of hippocampal neurons grown in dissociated cell culture. B-50 was detected with pre- and post-embedding immunoincubation, using affinity-

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::941f95628c67c08fb052a2180eb5852b
https://pubmed.ncbi.nlm.nih.gov/1385647

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B-50/GAP43 localization in polarized hippocampal neurons in vitro: an ultrastructural quantitative study

Autor: M. Van Lookeren Campagne, A.J. Verkleij, C.G. Dotti, W.H. Gispen, E.R.A. Jap Tjoen San, A.B. Oestreicher

Publikováno v: Neuroscience, 50, 35. Pergamon Press

Hippocampal pyramidal neurons cultured in vitro gradually develop morphologically and biochemically distinct axons and dendrites, resulting in functional neuronal polarization [Dotti C. G. et al. (1988) J. Neurosci. 8, 1454-1468]. We have studied the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5435d626e94527390c79f4b32f0acadc
https://dspace.library.uu.nl/handle/1874/3604

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Ultrastructural localization of adrenocorticotropic hormone and the phosphoprotein B 50/growth-associated protein 43 in freeze-substituted, Lowicryl HM20 embedded mesencephalic central gray substance of the rat

Autor: A.J. Verkleij, A.B. Oestreicher, M. Van Lookeren Campagne, P. Buma, W.H. Gispen

Publikováno v: Neuroscience, 42, 517. Pergamon Press

Previous studies have shown that the endogenous phosphorylation of the neuron-specific protein B-50 in isolated synaptic plasma membranes is inhibited by adrenocorticotrophic hormone(1–24). The aim of this study is to examine if there is a specific

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::05209bb63d385142b43db0c063dd7b7e
https://dspace.library.uu.nl/handle/1874/4149

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