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pro vyhledávání: '"A.E. Kellie"'
Autor:
A.E. Kellie
Publikováno v:
Journal of Steroid Biochemistry. 6:277-281
The determination of steroid hormones and their metabolites by radioimmunoassay is now well established. Conventionally the steroid is converted into a derivative which introduces a carboxylic acid group and this group is joined covalently as a hapte
Autor:
Herman Adlercreutz, A.E. Kellie, G. Braissand, W. P. Collins, J. Brown, J. Spieler, H. Campbell, U. Goebelsman
Publikováno v:
Journal of Steroid Biochemistry. 17:695-702
The concentrations of various estrogen glucuronides and pregnanediol-3α-glucuronide have been measured in daily samples of early morning urine (EMU) and 24 h pooled collections throughout a total of 70 menstrual cycles (58 subjects). The immediate o
Publikováno v:
Journal of Steroid Biochemistry. 13:359-362
The 17,20α-isopropylidenedioxy derivatives of 5β-pregnane-3α,17,20α-triol and 5β-pregn-6-ene-3α,17,20α-triol have been treated with methylacetobromoglucuronate and silver oxide to give the corresponding 3α-β- d -glucuronide triacetate methyl
Autor:
A.E. Kellie, Padmananda Samarajeewa
Publikováno v:
Journal of Steroid Biochemistry. 22:843-849
The hapten (5 beta-pregnane-17,20 alpha-diol-3 alpha-yl glucuronide) required to develop the radioimmunoassay was synthesized by an unambiguous chemical synthesis. An immunogenic complex was synthesized by coupling bovine serum albumin (BSA) to the g
Publikováno v:
Journal of Steroid Biochemistry. 6:91-94
Two anti-oestradiol sera have been studied with respect to their ability to bind free oestrogens, oestrogen derivatives and oestrogen conjugates. Anti-oestradiol-6-O(carboxymethyl)-oxime serum bound free oestradiol with high specificity but did not c
Publikováno v:
Journal of Steroid Biochemistry. 11:1165-1171
An antibody was prepared against pregnanediol-3α-glucuronide-BSA. The hapten (5β-pregnane-20α-ol-3α-yl-glucuronide) used for the preparation of the immunogen and as ‘cold’ standard for RIA was synthesized by an unambiguous chemical synthesis.
Publikováno v:
Journal of Steroid Biochemistry. 2:1-10
A sensitive procedure for the determination of oestradiol in small plasma samples by competitive-binding to uterine supernatant preparations has been described. Although preparations from other species were examined, uterine supernatants from New Zea
Autor:
Janet M. Oliver, A.E. Kellie
Publikováno v:
Biochemical Journal. 119:187-191
1. Techniques have been developed to measure the concentrations of the ribonucleotides of the immature rat uterus in vivo. Tissue was frozen rapidly in liquid nitrogen, ground to a fine powder, dispersed in frozen perchloric acid and thawed slowly. N
Publikováno v:
British Journal of Cancer
An assay is described for measuring the concentration of specific, high-affinity oestradiol receptors in the cell supernatant fraction of breast tumour biopsies. The method has been applied to biopsies from 94 patients with malignant and benign disea
Publikováno v:
Journal of Steroid Biochemistry. 3:275-288
A preliminary study has been made of the possibility of raising antisera to steroid glucuronosides. Using steroid glucuronosides as haptens, macromolecular complexes have been prepared by joining the carboxylic acid group of steroid conjugates covale