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Body fluid identification using a targeted mRNA massively parallel sequencing approach - results of a EUROFORGEN/EDNAP collaborative exercise

Publikováno v: Ingold, S, Dørum, G, Hanson, E, Berti, A, Branicki, W, Brito, P, Elsmore, P, Gettings, K B, Giangasparo, F, Gross, T E, Hansen, S, Hanssen, E N, Kampmann, M-L, Kayser, M, Laurent, F-X, Morling, N, Mosquera-Miguel, A, Parson, W, Phillips, C, Porto, M J, Pośpiech, E, Roeder, A D, Schneider, P M, Johann, K S, Steffen, C R, Syndercombe-Court, D, Trautmann, M, van den Berge, M, van den Gaag, K J, Vannier, J, Verdoliva, V, Vidaki, A, Xavier, C & Ballantyne, J 2018, ' Body fluid identification using a targeted mRNA massively parallel sequencing approach – results of a EUROFORGEN/EDNAP collaborative exercise ', Forensic Science International-Genetics . https://doi.org/10.1016/j.fsigen.2018.01.002
Forensic Science International: Genetics, 34, 105-115. Elsevier Ireland Ltd

In a previous study we presented an assay for targeted mRNA sequencing for the identification of human body fluids, optimised for the Illumina MiSeq/FGx MPS platform. This assay, together with an additional in-house designed assay for the Ion Torrent

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1e483f9cf55d093d770c8dde11f8ebae
https://pure.eur.nl/en/publications/71c74d70-7ee4-4400-87b8-eedd32257e64

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Evaluation of genetic markers for forensic identification of human body fluids

Autor: O.A. Afolabi, A.D. Roeder, Arati Iyengar, Ss Hadi

Publikováno v: Forensic Science International: Genetics Supplement Series. 6:e241-e243

Identification of body fluids deposited at crime scenes can often become challenging due to their small size, poor visibility to the naked eye and the effect of environmental insults. Traditionally, body fluids are identified using techniques that ar

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::39237d1f2a4c6255bcabef583905e4a4
https://doi.org/10.1016/j.fsigss.2017.09.098

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RNA/DNA co-analysis from human skin and contact traces – results of a sixth collaborative EDNAP exercise

Publikováno v: Forensic Science International: Genetics. 16:139-147

The European DNA profiling group (EDNAP) organized a sixth collaborative exercise on RNA/DNA co-analysis for body fluid/tissue identification and STR profiling. The task was to identify skin samples/contact traces using specific RNA biomarkers and te

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9b7159e56de28c28174f04ef805c59ee
https://doi.org/10.1016/j.fsigen.2015.01.002

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Reference gene study for forensic body fluid identification

Autor: A.D. Roeder, Arati Iyengar, O.A. Afolabi, Ss Hadi

Publikováno v: Forensic Science International: Genetics Supplement Series. 5:e167-e169

Reference genes are used in forensic body fluid identification studies to normalise data generated during gene expression experiments. The use of reference genes improves the reliability of qRT-PCR. In this study, 10 most common reference genes UCE,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::5edf4687dfe8e4cb0293d35c136a5a5d
https://doi.org/10.1016/j.fsigss.2015.09.067

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Body Fluid Identification Using mRNA Profiling

Autor: Cordula Haas, A.D. Roeder

Publikováno v: Methods in Molecular Biology ISBN: 9781493935956

RNA analysis is a valuable tool for the identification of the forensically relevant body fluids, saliva, blood, menstrual blood, cervicovaginal fluid, and semen. Multiple human mRNA and bacterial RNA markers have been identified for each of these bod

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::f90b02b5def08951c81c13ce9e378172
https://doi.org/10.1007/978-1-4939-3597-0_2

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Purification of PCR Products to Improve STR Profiles

Autor: A.D. Roeder

Publikováno v: Methods in Molecular Biology ISBN: 9781493935956

Forensic laboratories routinely perform STR analyses using commercially available STR kits. Very low levels of DNA are extracted from many forensic samples. In these samples, the amount of DNA that can be placed in a PCR is below the optimal DNA rang

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::0efa3bf2657861fc69e6f175d4a01bc3
https://doi.org/10.1007/978-1-4939-3597-0_10

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RNA/DNA co-analysis from human menstrual blood and vaginal secretion stains: results of a fourth and fifth collaborative EDNAP exercise

Publikováno v: Haas, C, Hanson, E, Anjos, M J, Ballantyne, K N, Banemann, R, Bhoelai, B, Borges, E, Carvalho, M, Courts, C, De Cock, G, Drobnic, K, Dötsch, M, Fleming, R, Franchi, C, Gomes, I, Hadzic, G, Harbison, S A, Harteveld, J, Hjort, B B, Hollard, C, Hoff-Olsen, P, Hüls, C, Keyser, C, Maroñas, O, McCallum, N, Moore, D, Morling, N, Niederstätter, H, Noël, F, Parson, W, Phillips, C, Popielarz, C, Roeder, A D, Salvaderi, L, Sauer, E, Schneider, P M, Shanthan, G, Court, D S, Turanská, M, van Oorschot, R A H, Vennemann, M, Vidaki, A, Zatkalíková, L & Ballantyne, J 2014, ' RNA/DNA co-analysis from human menstrual blood and vaginal secretion stains : Results of a fourth and fifth collaborative EDNAP exercise ', Forensic science international. Genetics, vol. 8, no. 1, pp. 203-12 . https://doi.org/10.1016/j.fsigen.2013.09.009

The European DNA Profiling Group (EDNAP) organized a fourth and fifth collaborative exercise on RNA/DNA co-analysis for body fluid identification and STR profiling. The task was to identify dried menstrual blood and vaginal secretion stains using spe

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a53762b0d4bb27a57ca6511b94107555
https://pubmed.ncbi.nlm.nih.gov/24315610

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RNA/DNA co-analysis from human saliva and semen stains--results of a third collaborative EDNAP exercise

Publikováno v: Haas, C, Hanson, E, Anjos, M J, Banemann, R, Berti, A, Borges, E, Carracedo, A, Carvalho, M, Courts, C, De Cock, G, Dötsch, M, Flynn, S, Gomes, I, Hollard, C, Hjort, B B, Hoff-Olsen, P, Hríbiková, K, Lindenbergh, A, Ludes, B, Maroñas, O, McCallum, N, Moore, D, Morling, N, Niederstätter, H, Noel, F, Parson, W, Popielarz, C, Rapone, C, Roeder, A D, Ruiz, Y, Sauer, E, Schneider, P, Sijen, T, Court, D S, Sviežená, B, Turanská, M, Vidaki, A, Zatkalíková, L & Ballantyne, J 2013, ' RNA/DNA co-analysis from human saliva and semen stains--results of a third collaborative EDNAP exercise ', Forensic science international. Genetics, vol. 7, no. 2, pp. 230-9 . https://doi.org/10.1016/j.fsigen.2012.10.011

A third collaborative exercise on RNA/DNA co-analysis for body fluid identification and STR profiling was organized by the European DNA Profiling Group (EDNAP). Twenty saliva and semen stains, four dilution series (10-0.01 μl saliva, 5-0.01 μl seme

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::44b788dc6331e5c85db502aeb29a7793
https://curis.ku.dk/ws/files/128001063/RNA_DNA_co_analysis_from_human_saliva_and_semen_stains_Results_of_a_third_collaborative_EDNAP_exercise_2013_Forensic_Science_International_Genetics.pdf

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mRNA profiling using a minimum of five mRNA markers per body fluid and a novel scoring method for body fluid identification

Autor: A.D. Roeder, Cordula Haas

Publikováno v: International journal of legal medicine. 127(4)

Messenger RNA (mRNA) expression varies among cell types; therefore, analyses for the presence of particular mRNAs can be used to identify biological fluids in forensic samples. For this work, several novel markers were characterized for saliva, cervi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e1872870ac80074231d5f5705d2ad706
https://pubmed.ncbi.nlm.nih.gov/23254458

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