Dynamics of Nakaseomyces glabratus biofilm formation

Autor: E. M. Lenchenko, N. P. Sachivkina, A. V. Liseitsev

Publikováno v: Ветеринария сегодня, Vol 13, Iss 3, Pp 269-274 (2024)

Formation of biofilms of microorganisms, including those of Nakaseomyces glabratus, is responsible for the development of local and systemic pathologies in humans and animals. The system of gene expression coordination (quorum sensing) in the represe

Externí odkaz: https://doaj.org/article/2218da22e16145ae8c40db7d8b441f27

CasPER, a method for directed evolution in genomic contexts using mutagenesis and CRISPR/Cas9

Autor: Alicia V. Lis, Lasse Ebdrup Pedersen, Michael Krogh Jensen, Tadas Jakočiūnas, Jay D. Keasling

Publikováno v: Jakočiūnas, T, Pedersen, L E, Lis, A V, Jensen, M K & Keasling, J D 2018, ' CasPER, a method for directed evolution in genomic contexts using mutagenesis and CRISPR/Cas9 ', Metabolic Engineering, vol. 48, pp. 288-296 . https://doi.org/10.1016/j.ymben.2018.07.001

Here we describe a method for robust directed evolution using mutagenesis of large sequence spaces in their genomic contexts. The method employs error-prone PCR and Cas9-mediated genome integration of mutant libraries of large-sized donor variants in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7c8d5a69d2fbe063a4037eb7e9e7458c
https://doi.org/10.1016/j.ymben.2018.07.001

The analysis of relative movements of consolidations’ mating elements of the interroller wedge-shaped gaps rolling tunnelers of wells

Autor: V. . Lis

Publikováno v: Вестник СибАДИ, Vol 0, Iss 1(41), Pp 24-30 (2017)

The article dwells on the mechanics of relative movement of mating elements of flanged type’s consolidations of wedge-shaped interroller end clearances. There is considered kinematics of interaction process of consolidations’ elements of spherica

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doajarticles::df4e1f2c7109d812f4f53898be7bb374
https://vestnik.sibadi.org/jour/article/view/96

Exploring small-scale chemostats to scale up microbial processes: 3-hydroxypropionic acid production in S. cerevisiae

Autor: Tobias Klein, Jay D. Keasling, Konstantin Schneider, Alicia V. Lis, Jost Weber, Michael Krogh Jensen

Publikováno v: Microbial cell factories, vol 18, iss 1
Microbial Cell Factories
Microbial Cell Factories, Vol 18, Iss 1, Pp 1-11 (2019)
Lis, A V, Schneider, K, Weber, J, Keasling, J D, Jensen, M K & Klein, T 2019, ' Exploring small-scale chemostats to scale up microbial processes: 3-hydroxypropionic acid production in S. cerevisiae ', Microbial Cell Factories, vol. 18, no. 1, 50 . https://doi.org/10.1186/s12934-019-1101-5

BackgroundThe physiological characterization of microorganisms provides valuable information for bioprocess development. Chemostat cultivations are a powerful tool for this purpose, as they allow defined changes to one single parameter at a time, whi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::53de78a96838bb7998b85e6cec1e7fc6
https://doi.org/10.1186/s12934-019-1101-5

Use of FDSS/μCell imaging platform for preclinical cardiac electrophysiology safety screening of compounds in human induced pluripotent stem cell-derived cardiomyocytes

Autor: Armando Lagrutta, Maria I. Roman, Haoyu Zeng, E V Lis, Frederick Sannajust

Publikováno v: Journal of Pharmacological and Toxicological Methods. 81:217-222

FDSS/μCell is a high-speed acquisition imaging platform (Hamamatsu Ltd., Hamamatsu, Japan) that allows for simultaneous high-throughput reading under controlled conditions. We evaluated the Ca 2 + transients or optical membrane potential changes of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0562e349143aeb72cd279f94112f12c6
https://doi.org/10.1016/j.vascn.2016.05.009