Zobrazeno 1 - 10
of 11
pro vyhledávání: '"A. S. I. Jaikaran"'
Autor:
Anna S. I. Jaikaran, Jeffrey Youn, Karl Z. Demmans, Voula Kanelis, Ryan M. Woloschuk, Maruti Uppalapati, P. Maximilian M. Reed, G. Andrew Woolley
Publikováno v:
Protein Sci
Photo‐control of affinity reagents offers a general approach for high‐resolution spatiotemporal control of diverse molecular processes. In an effort to develop general design principles for a photo‐controlled affinity reagent, we took a structu
Autor:
Danlin Zhen, Vitali Borisenko, Katherine E. Brechun, Wouter D. Hoff, Andrew Woolley, Masato Kumauchi, Katja M. Arndt, Anna S. I. Jaikaran
Publikováno v:
Biochemistry. 58:2682-2694
We report the design and characterization of photoactive yellow protein (PYP)-blue fluorescent protein (mTagBFP) fusion constructs that permit the direct assay of reconstitution and function of the PYP domain. These constructs allow for in vivo testi
Autor:
Kristin Blacklock, Nobuo Yasuike, Maruti Uppalapati, Sherin McDonald, Sagar D. Khare, Anna S. I. Jaikaran, Huixin Lu, G. Andrew Woolley
Publikováno v:
ChemPhotoChem
Photo-controlled affinity reagents seek to provide modular spatiotemporal control of bioactivity by conferring photo-switchability of function on an affinity reagent scaffold. Here we used Rosetta-based computational methods to screen for sites on th
Autor:
Mostafizur Mazumder, Michael Altmann, Xiuling Xu, Eric K Leis, Alan Cochrane, G. Andrew Woolley, Huixin Lu, Anna S. I. Jaikaran, Anil Kumar
Publikováno v:
ACS Synthetic Biology. 8:744-757
The precise spatiotemporal regulation of protein synthesis is essential for many complex biological processes such as memory formation, embryonic development and tumor formation. Current methods used to study protein synthesis offer only a limited de
Autor:
Ryan M. Woloschuk, Xiuling Xu, Maruti Uppalapati, Frederick S. Vizeacoumar, Anna S. I. Jaikaran, G. Andrew Woolley, Jakeb M. Reis, Sherin McDonald
Publikováno v:
ACS synthetic biology. 7(10)
Nature provides an array of proteins that change conformation in response to light. The discovery of a complementary array of proteins that bind only the light-state or dark-state conformation of their photoactive partner proteins would allow each li
Autor:
Katherine E. Brechun, Stacy-Anne Morgan, Yih-Yang Chen, Helen Y. Fan, G. Andrew Woolley, Anna S. I. Jaikaran
Publikováno v:
Biochemistry. 50:1226-1237
Photocontrolled transcription factors could be powerful tools for probing the roles of transcriptional processes in a variety of settings. Previously, we designed a photocontrolled DNA-binding protein based on a fusion between the bZIP region of GCN4
Autor:
Sergey N. Krylov, Janet R. Kumita, Joseph P. Calarco, Anna S. I. Jaikaran, Maxim V. Berezovski, G. Andrew Woolley, Oliver S. Smart
Publikováno v:
Biochemistry. 45:6075-6084
Synthetic photocontrolled proteins could be powerful tools for probing cellular chemistry. Several previous attempts to produce such systems by incorporating photoisomerizable chromophores into biomolecules have led to photocontrol but with incomplet
Publikováno v:
Analytical Biochemistry. 307:252-257
Site-specific incorporation of biophysical probes into proteins during translation can permit structure/function studies on selected proteins in heterogeneous environments. We report here a procedure for incorporating a fluorescent tag into proteins
Publikováno v:
Scopus-Elsevier
The channel-forming properties of two analogs of gramicidin, gramicidin-ethylenediamine (gram-EDA), and gramicidin-N,N-dimethylethylenediamine (gram-DMEDA) were studied in planar lipid bilayers, using protons as the permeant ion. These peptides have
Publikováno v:
Journal of Biological Chemistry. 265:12503-12512
Germin was previously shown to contain covalently bonded and adventitious glycans. The object of the present investigation was to characterize the two types of glycan. The presence of N- but not O-glycans in germin is indicated by the biosynthesis of