Výsledky vyhledávání - "A. N. Wulfson"

Receptor-binding domain of ephrin-A1: Production in bacterial expression system and activity

Autor: A. N. Wulfson, Oksana V. Nekrasova, Alexey V. Feofanov, Mikhail P. Kirpichnikov, A. I. Tagvey, R. V. Tikhonov, Olga V. Bocharova, S.A. Yakimov, M. V. Astapova, Peter M. Kolosov, A. A. Korchagina, George V. Sharonov

Publikováno v: Biochemistry (Moscow). 77:1387-1394

Eph receptor tyrosine kinases and their ligands, the ephrins, perform an important regulatory function in tissue organization, as well as participate in malignant transformation of cells. Ephrin-A1, a ligand of A class Eph receptors, is a modulator o

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9bfc20a49fd86370e4573433303708d9
https://doi.org/10.1134/s0006297912120073

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A new hybrid protein for production of recombinant bacteriorhodopsin in Escherichia coli

Autor: Anna I. Tagvey, R. V. Tikhonov, Mikhail P. Kirpichnikov, S.A. Yakimov, A. N. Wulfson, Dmitry A. Dolgikh, Mikhail A. Ostrovsky, Oksana V. Nekrasova, Tatyana N. Simonova

Publikováno v: Journal of Biotechnology. 147:145-150

Unique properties of bacteriorhodopsin, namely, photochromism and high thermal stability, make this protein an attractive target for physico-chemical studies, as well as for various biotechnological applications. Using Mistic as a suitable carrier fo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ab6500cd5e8730549e5a60c7697d3b74
https://doi.org/10.1016/j.jbiotec.2010.03.019

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Bacterial production and refolding from inclusion bodies of a 'Weak' toxin, a disulfide rich protein

Autor: T. L. Ustich, Yu. N. Utkin, Mikhail P. Kirpichnikov, A. N. Wulfson, R. V. Tikhonov, Dmitry A. Dolgikh, Alexander S. Paramonov, A. S. Arseniev, Igor E. Kasheverov, Ekaterina N. Lyukmanova, Mikhail A. Shulepko, V. I. Tsetlin, Zakhar O. Shenkarev

Publikováno v: Biochemistry (Moscow). 74:1142-1149

The gene for the "weak" toxin of Naja kaouthia venom was expressed in Escherichia coli. "Weak" toxin is a specific inhibitor of nicotine acetylcholine receptor, but mechanisms of interaction of similar neurotoxins with receptors are still unknown. Sy

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7fd47d0df0276734e9ebd757c61c43c5
https://doi.org/10.1134/s0006297909100101

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Recombinant human insulin IX. Investigation of factors, influencing the folding of fusion protein-S-sulfonates, biotechnological precursors of human insulin

Autor: S.A. Yakimov, Irina A. Belacheu, Heloisa Tunes, Luciano Vilela, A. N. Wulfson, R. V. Tikhonov, Pechenov Se, Josef Ernst Thiemann, Vadim E Klyushnichenko

Publikováno v: Protein Expression and Purification. 26:187-193

The peculiarities of molecular structures and the influence of reaction conditions on the folding efficiency of fusion proteins-biotechnological precursors of human insulin, expressed in Escherichia coli as inclusion bodies have been investigated. Th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::53c3fb010a60ce7d13bd0e819e545b75
https://doi.org/10.1016/s1046-5928(02)00531-4

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Methods for Preparation of Recombinant Cytokine Proteins V. Mutant Analogues of Human Interferon-γ with Higher Stability and Activity

Autor: Vladimir P. Kuznetzov, R. V. Tikhonov, Pechenov Se, Korobko Vyacheslav G, Dmitriy L Beliaev, L. N. Shingarova, A. N. Wulfson, Vitaliy I Shvetz, S.A. Yakimov, Vadim E Klyushnichenko, Alla A Babajantz

Publikováno v: Protein Expression and Purification. 24:173-180

Mutant analogues of recombinant human immune interferon (IFN-gamma) with higher stability and biological activity were prepared. Depending on the analogue, protein structure modification might involve introduction of an intramonomer disulfide bond (t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::115131ace840775fe88cda6200392bd7
https://doi.org/10.1006/prep.2001.1565

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Recombinant Human Insulin

Autor: R. V. Tikhonov, Pechenov Se, A. N. Wulfson, Heloisa Tunes, Josef Ernst Thiemann, Elena F. Boldireva, Irina A. Belacheu, Vadim E Klyushnichenko, Luciano Vilela, Korobko Vyacheslav G, S.A. Yakimov

Publikováno v: Protein Expression and Purification. 21:176-182

Various methods have been investigated for the isolation and purification of fusion proteins of precursors of human insulin in the form of S-sulfonates, from the biomass of transformed Escherichia coli cells. Fusion proteins were prepared with differ

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::1d389ae5ce5b1d0402cff323da395575
https://doi.org/10.1006/prep.2000.1345

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[Untitled]

Autor: Roman S. Esipov, R. V. Tikhonov, A. N. Wulfson, Pechenov Se, V. I. Shvets, A. I. Gurevich

Publikováno v: Russian Journal of Bioorganic Chemistry. 27:34-38

Renaturation of recombinant human interleukin-3 produced as inclusion bodies in the transformed cells of Escherichia coli was studied and optimized. Importance was shown of removing from the protein solution the hydrophobic cellular components causin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::c99d1e3f22809542e1efa6c3998b3755
https://doi.org/10.1023/a:1009527018260

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Determination of indole alkaloids from R. serpentina and R. vomitoria by high-performance liquid chromatography and high-performance thin-layer chromatography

Autor: I. N. Kuzovkina, A. N. Wulfson, V.E. Klyushnichenko, T.P. Tuzova, A. I. Miroshnikov, S.A. Yakimov, Ya. V. Syagailo

Publikováno v: Journal of Chromatography A. 704:357-362

The separation of a model mixture of six indole alkaloids by high-performance liquid chromatography (HPLC) and high-performance thin-layer chromatography (HPTLC) on the normal- and reversed-phase Armsorb supports, respectively, was studied. A procedu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::bfa680a0665704fe501396a4f0da2df2
https://doi.org/10.1016/0021-9673(95)00082-x

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Recombinant human insulin - II. Size-exclusion HPLC of biotechnological precursors. Factors influencing on retention and selectivity

Autor: A. N. Wulfson, V. E. Klyushnichenko

Publikováno v: Pure and Applied Chemistry. 65:2265-2272

The mechanism of exclusion-sorption interaction of insulin-containing proteins with the column support in accordance with the chemical and three-dimensional structure in native and denaturating conditions was observed. The mechanisms of weak adsorpti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::674146a5e682597240243df22c5eb2d2
https://doi.org/10.1351/pac199365102265

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