Zobrazeno 1 - 10
of 44
pro vyhledávání: '"A. Miller-Faurès"'
Publikováno v:
Nucleic Acids Research; 1/15/1980, Vol. 8 Issue 2, p377-387, 11p
Publikováno v:
Nucleic Acids Research. 8:377-387
Both the inhibitory effect of aphidicolin on the replicative alpha-polymerase and the reversibility of its action in vivo (Pedrali-Noy & Spadari, 1979, Biochem. Biophys. Res. Commun. 88, 1194-2002) allow the synchronization of cells in culture. Aphid
Autor:
A. Miller-Faurès, Jean Rommelaere
Publikováno v:
Journal of Molecular Biology. 98:195-218
When the nuclear DNA of Chinese hamster cells grown in the presence of bromo-deoxyuridine for one or less generation is analysed in an alkaline CsCl density gradient, a small fraction of replicated DNA can be found at densities intermediate between t
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects. 72:209-216
HeLa cells have been submitted to short tritiated nucleoside pulses, and their radioactivity has been followed in the nucleus minus nucleolus (N), in the nucleolus (n) and in the cytoplasm (C) after various incubation times in unlabeled media. The in
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects. 80:39-51
A study of the sedimentation pattern in sucrose gadients of rapidly- labeled RNA (5--30 min labeling with STAC/sup 14/! adenine) was done on whole extracts of HeLa cells. Two regions of radioactivity were found: a lighter region sedimenting in the ra
Publikováno v:
European Journal of Biochemistry. 3:57-69
HeLa cells were labelled for 10 or 30 minutes with [3H]thymidine and the DNA fractionated with chloroform-isoamylalcohol; almost 90% of the labelled DNA remained in the interphase whilst about 60% of total DNA (bulk) was extracted in the aqueous phas
Publikováno v:
Biochimica et biophysica acta. 340(4)
The kinetics and dose dependence were determined both for the repair synthesis and for the induction and repair of single-strand breaks in the 5-bromodeoxyuridine-substituted DNA of ultraviolet-irradiated Chinese hamster cells. A close temporal relat
Publikováno v:
Developments in biological standardization. 60
Applying Hydroxyurea, Nocodazole and Aphidicolin in succession to obtain parasynchronous growth, the progression of HTC and HeLa cells through the cell cycle has been monitored by laser flow cytometry. The experimental results show that HTC cells beh
Autor:
A C, Miller, A, Miller-Faurès
Publikováno v:
Developments in biological standardization. 50
Although procaryotes such as E. Coli are generally considered to be ideal hosts, able to amplify eucaryotic gene sequences contained within hybrid DNA plasmid molecules (3, 14), recent experimental evidence such as the decreased bacterial viability o
Publikováno v:
Biochimica et biophysica acta. 65
Three methods of isolation of nuclei of HeLa cells were investigated in connection with studies of pulse-labeled RNA in HeLa cells: (a) sucrose-homogenization, (b) osmotic shock, (c)_citric acid. Of the three methods only the nuclei isolated by citri