A novel three step protocol to isolate extracellular vesicles from plasma or cell culture medium with both high yield and purity

Autor: Xiaogang Zhang, Ellen G. F. Borg, A. Manuel Liaci, Harmjan R. Vos, Willem Stoorvogel

Publikováno v: Journal of Extracellular Vesicles, Vol 9, Iss 1 (2020)

Extracellular vesicles (EV) are membrane encapsulated nanoparticles that can function in intercellular communication, and their presence in biofluids can be indicative for (patho)physiological conditions. Studies aiming to resolve functionalities of

Externí odkaz: https://doaj.org/article/f330d1112c45446bbe345624f3e3ca6e

Unexpected moves

Autor: Susanne R Bruekner, Wietske Pieters, Alexander Fish, A Manuel Liaci, Serge Scheffers, Emily Rayner, Daphne Kaldenbach, Lisa Drost, Marleen Dekker, Sandrine van Hees-Stuivenberg, Elly Delzenne-Goette, Charlotte de Konink, Hellen Houlleberghs, Hendrikus Jan Dubbink, Abeer AlSaegh, Niels de Wind, Friedrich Förster, Hein te Riele, Titia K Sixma

Publikováno v: Nucleic Acids Research, 51(3), 1173-1188. Oxford University Press
Bruekner, S R, Pieters, W, Fish, A, Liaci, A M, Scheffers, S, Rayner, E, Kaldenbach, D, Drost, L, Dekker, M, van Hees-Stuivenberg, S, Delzenne-Goette, E, de Konink, C, Houlleberghs, H, Dubbink, H J, Alsaegh, A, de Wind, N, Förster, F, te Riele, H & Sixma, T K 2023, ' Unexpected moves : a conformational change in MutSα enables high-affinity DNA mismatch binding ', Nucleic Acids Research, vol. 51, no. 3, pp. 1173-1188 . https://doi.org/10.1093/nar/gkad015

The DNA mismatch repair protein MutSα recognizes wrongly incorporated DNA bases and initiates their correction during DNA replication. Dysfunctions in mismatch repair lead to a predisposition to cancer. Here, we study the homozygous mutation V63E in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b261243e65c36c88dc6a5fefdea17ff9
https://doi.org/10.1093/nar/gkad015

Human adenovirus 52 uses sialic acid-containing glycoproteins and the coxsackie and adenovirus receptor for binding to target cells.

Autor: Annasara Lenman, A Manuel Liaci, Yan Liu, Carin Årdahl, Anandi Rajan, Emma Nilsson, Will Bradford, Lisa Kaeshammer, Morris S Jones, Lars Frängsmyr, Ten Feizi, Thilo Stehle, Niklas Arnberg

Publikováno v: PLoS Pathogens, Vol 11, Iss 2, p e1004657 (2015)

Most adenoviruses attach to host cells by means of the protruding fiber protein that binds to host cells via the coxsackievirus and adenovirus receptor (CAR) protein. Human adenovirus type 52 (HAdV-52) is one of only three gastroenteritis-causing HAd

Externí odkaz: https://doaj.org/article/b1e2d6413f6743469d9e2b2da13aa229

Ex Vivo and In Vivo CD46 Receptor Utilization by Species D Human Adenovirus Serotype 26 (HAdV26).

Autor: Hemsath, Jack R.¹, Manuel Liaci, A.^2,3, Rubin, Jeffrey D.⁴, Parrett, Brian J.⁴, Shao-Chia Lu⁴, Nguyen, Tien V.⁵, Turner, Mallory A.⁴, Chen, Christopher Y.⁵, Cupelli, Karolina², Reddy, Vijay S.⁶, Stehle, Thilo², Kathryn Liszewski, M.⁷, Atkinson, John P.⁷, Barry, Michael A.^5,8,9 mab@mayo.edu

Publikováno v: Journal of Virology. Feb2022, Vol. 96 Issue 3, p1-14. 14p.

Ganglioside and Non-Ganglioside Mediated Host Responses to the Mouse Polyomavirus

Autor: Robert L. Garcea, Ursula Neu, Samantha D. O’Hara, Thilo Stehle, A. Manuel Liaci, Michael H. C. Buch

Publikováno v: PLoS Pathogens, Vol 11, Iss 10, p e1005104 (2015)
'PLoS Pathogens ', vol: 11, pages: e1005104-1-e1005104-22 (2015)
PLoS Pathogens

Murine polyomavirus (MuPyV) causes tumors of various origins in newborn mice and hamsters. Infection is initiated by attachment of the virus to ganglioside receptors at the cell surface. Single amino acid exchanges in the receptor-binding pocket of t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cad12092a72ae653768e7561755ed5c3
https://doaj.org/article/7acafed21aeb41ab882d43c39a8161b2