Zobrazeno 1 - 10
of 29
pro vyhledávání: '"A. G. Lezius"'
Autor:
Axel G. Lezius, Henning J. Hachmann
Publikováno v:
European Journal of Biochemistry. 50:357-366
Cytoplasmic (high-molecular-weight) DNA polymerase was partially purified from mouse myeloma. Upon chromatography on DEAE-Sephadex, following fractionation on phosphocellulose, the enzyme was resolved into three species named CI, CII, and CIII. The s
Publikováno v:
European Journal of Biochemistry. 60:51-55
1. RNA polymerase from Escherichia coli is selectively and strongly retained by a heparin-substituted agarose and can be eluted therefrom by a neutral buffer containing 0.6 M salt. The method is applicable to relatively crude preparations of the enzy
Publikováno v:
Nucleic Acids Research. 5:1955-1970
High resolution nuclear magnetic resonance (NMR) and ethidium bromide binding studies are used to demonstrate that poly d(G-T) forms an ordered double helical structure at low temperatures (below 24 degrees C in 0.3 M NaCl) in which G and T are hydro
Publikováno v:
Biopolymers. 13:1751-1756
Microcalorimetric heat capacity measurements on dilute and concentrated solutions and films of poly[d(A-T)·d(A-T)] in 2 M sodium chloride have been carried out. Values for enthalpy, entropy, and temperature of the helix–coil transition have been f
Autor:
Karl Heinz Scheit, Axel G. Lezius
Publikováno v:
European Journal of Biochemistry. 3:85-94
When 4-thio-thymidine-5′-triphosphate is substituted for dTTP in the enzymatic synthesis of poly d(A-T) by DNA polymerase, formation of a polymer is observed either by incorporation of radioactive labelled deoxyadenosine nucleotide or by a hypochro
Autor:
Axel G. Lezius, Ute Rath
Publikováno v:
European Journal of Biochemistry. 24:163-167
Two bacterial DNA polymerases lacking the 5′-exonuclease, isolated from Escherichia coli and Bacillus subtilis are compared with respect to their capability to synthesize the nucleoside analong containing polydeoxynucleotide poly[d(A-s4T) · d(A-s4
Autor:
A G Lezius, H A Barker
Publikováno v:
Biochemistry. 4:510-518
Autor:
Axel G. Lezius
Publikováno v:
European Journal of Biochemistry. 14:154-160
Starting from 2-thiodeoxythymidine, the mono- and triphosphate of the nucleoside analog were prepared by enzymatic phosphorylation with thymidine kinase and thymidylate kinase, respectively. 2-Thiodeoxythymidine triphosphate was also obtained by chem
Autor:
Axel G. Lezius, Marie-Thérèse Sarocchi, Christian Marck, Danielle Thiele, Wilhelm Guschlbauer
Publikováno v:
Molecular Biology Reports. 1:155-160
The CD spectra of three polydeoxyribonucleotide complexes, poly(dG-C). poly(dG-C), poly(dA-G). poly(dT-C) and poly(dA-C). poly(dT-G) have been studied as a function of pH at 25°C. Only poly(dA-G). poly(dT-C) showed large hysteresis, analogous to tha
Autor:
Axel G. Lezius
Publikováno v:
Hoppe-Seyler´s Zeitschrift für physiologische Chemie. 352:491-500