Sequencing by avidity enables high accuracy with low reagent consumption

Autor: Sinan Arslan, Francisco J. Garcia, Minghao Guo, Matthew W. Kellinger, Semyon Kruglyak, Jake A. LeVieux, Adeline H. Mah, Haosen Wang, Junhua Zhao, Chunhong Zhou, Andrew Altomare, John Bailey, Matthew B. Byrne, Chiting Chang, Steve X. Chen, Byungrae Cho, Claudia N. Dennler, Vivian T. Dien, Derek Fuller, Ryan Kelley, Omid Khandan, Michael G. Klein, Michael Kim, Bryan R. Lajoie, Bill Lin, Yu Liu, Tyler Lopez, Peter T. Mains, Andrew D. Price, Samantha R. Robertson, Hermes Taylor-Weiner, Ramreddy Tippana, Austin B. Tomaney, Su Zhang, Minna Abtahi, Mark R. Ambroso, Rosita Bajari, Ava M. Bellizzi, Chris B. Benitez, Daniel R. Berard, Lorenzo Berti, Kelly N. Blease, Angela P. Blum, Andrew M. Boddicker, Leo Bondar, Chris Brown, Chris A. Bui, Juan Calleja-Aguirre, Kevin Cappa, Joshua Chan, Victor W. Chang, Katherine Charov, Xiyi Chen, Rodger M. Constandse, Weston Damron, Mariam Dawood, Nicole DeBuono, John D. Dimalanta, Laure Edoli, Keerthana Elango, Nikka Faustino, Chao Feng, Matthew Ferrari, Keith Frankie, Adam Fries, Anne Galloway, Vlad Gavrila, Gregory J. Gemmen, James Ghadiali, Arash Ghorbani, Logan A. Goddard, Adriana Roginski Guetter, Garren L. Hendricks, Jendrik Hentschel, Daniel J. Honigfort, Yun-Ting Hsieh, Yu-Hsien Hwang Fu, Scott K. Im, Chaoyi Jin, Shradha Kabu, Daniel E. Kincade, Shawn Levy, Yu Li, Vincent K. Liang, William H. Light, Jonathan B. Lipsher, Tsung-li Liu, Grace Long, Rui Ma, John M. Mailloux, Kyle A. Mandla, Anyssa R. Martinez, Max Mass, Daniel T. McKean, Michael Meron, Edmund A. Miller, Celyne S. Moh, Rachel K. Moore, Juan Moreno, Jordan M. Neysmith, Cassandra S. Niman, Jesus M. Nunez, Micah T. Ojeda, Sara Espinosa Ortiz, Jenna Owens, Geoffrey Piland, Daniel J. Proctor, Josua B. Purba, Michael Ray, Daisong Rong, Virginia M. Saade, Sanchari Saha, Gustav Santo Tomas, Nicholas Scheidler, Luqmanal H. Sirajudeen, Samantha Snow, Gudrun Stengel, Ryan Stinson, Michael J. Stone, Keoni J. Sundseth, Eileen Thai, Connor J. Thompson, Marco Tjioe, Christy L. Trejo, Greg Trieger, Diane Ni Truong, Ben Tse, Benjamin Voiles, Henry Vuong, Jennifer C. Wong, Chiung-Ting Wu, Hua Yu, Yingxian Yu, Ming Yu, Xi Zhang, Da Zhao, Genhua Zheng, Molly He, Michael Previte

Publikováno v: Nature Biotechnology.

We present avidity sequencing, a sequencing chemistry that separately optimizes the processes of stepping along a DNA template and that of identifying each nucleotide within the template. Nucleotide identification uses multivalent nucleotide ligands

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::134ecc28f29d15d85c9ac30057097e39
https://doi.org/10.1038/s41587-023-01750-7

Real-Time DNA Sequencing from Single Polymerase Molecules

Autor: Jeremy Gray, Mark Trulson, Patrick Marks, John Dixon, Ravindra V. Dalal, Fred Christians, Adrian Fehr, Jon M. Sorenson, Stephen Turner, Alfred Gaertner, Sonya Clark, Geoff Otto, Gregory J. Kearns, John Lyle, Alex DeWinter, Brad Bettman, Ronald Kuse, Primo Baybayan, Steven Lin, Denis Zaccarin, John Vieceli, Joy Roy, Cheryl Heiner, David R. Rank, Kevin Travers, Robert Sebra, Mathieu Foquet, Thang Pham, Dawn Wu, Keith Bjornson, Michael Phillips, Arkadiusz Bibillo, Bidhan Chaudhuri, Gene Shen, Alicia Yang, Mark Maxham, Peter Zhao, Khai Luong, Paul Hardenbol, Insil Park, Jonas Korlach, Paul Lundquist, Jeffrey Wegener, Kevin Hester, David P. Holden, Paul Peluso, Congcong Ma, Frank Zhong, Yves Lacroix, Austin B. Tomaney, Devon Murphy, John Eid, Xiangxu Kong, Ronald L. Cicero

Publikováno v: Science. 323:133-138

We present single-molecule, real-time sequencing data obtained from a DNA polymerase performing uninterrupted template-directed synthesis using four distinguishable fluorescently labeled deoxyribonucleoside triphosphates (dNTPs). We detected the temp

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4fcddf7f17aa6a34d664fd9d235e401b
https://doi.org/10.1126/science.1162986

MACHO Project Limits on Black Hole Dark Matter in the 1–30 [ITAL]M[/ITAL][TINF]⊙[/TINF] Range

Autor: C. Alcock, R. A. Allsman, D. R. Alves, T. S. Axelrod, A. C. Becker, D. P. Bennett, K. H. Cook, N. Dalal, A. J. Drake, K. C. Freeman, M. Geha, K. Griest, M. J. Lehner, S. L. Marshall, D. Minniti, C. A. Nelson, B. A. Peterson, P. Popowski, M. R. Pratt, P. J. Quinn, C. W. Stubbs, W. Sutherland, A. B. Tomaney, T. Vandehei, D. L. Welch, null (The MACHO Collaboration)

Publikováno v: The Astrophysical Journal. 550:L169-L172

We report on a search for long duration microlensing events towards the Large Magellanic Cloud. We find none, and therefore put limits on the contribution of high mass objects to the Galactic dark matter. At 95% confidence level we exclude objects in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::075ce281dfb58972ea7a945758c749c8
https://doi.org/10.1086/319636

The MACHO Project Large Magellanic Cloud Variable‐Star Inventory. IX. Frequency Analysis of the First‐Overtone RR Lyrae Stars and the Indication for Nonradial Pulsations

Autor: T. Vandehei, K. H. Cook, S. L. Marshall, William J. Sutherland, A. B. Tomaney, Marla Geha, D. L. Welch, David R. Alves, R. A. Allsman, Andrew J. Drake, P. Popowski, C. A. Nelson, Dante Minniti, Christopher W. Stubbs, Kenneth C. Freeman, David P. Bennett, Kim Griest, T. S. Axelrod, Christine M. Clement, Jason F. Rowe, A. W. Rodgers, Peter J. Quinn, Charles Alcock, Bruce A. Peterson, A. C. Becker, Mark Pratt, D. W. Kurtz, Géza Kovács, Matthew J. Lehner

Publikováno v: ASTROPHYSICAL JOURNAL
Artículos CONICYT
CONICYT Chile
instacron:CONICYT

More than 1300 variables classified provisionally as first overtone RR Lyrae pulsators in the MACHO variable star database of the Large Magellanic Cloud (LMC) have been subjected to standard frequency analysis. Based on the remnant power in the prewh

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e6e0c634cc905a6d1bd4c182c4b6654a
https://doi.org/10.1086/309530

Is the Large Magellanic Cloud Microlensing Due to an Intervening Dwarf Galaxy?

Autor: Mark Pratt, David R. Alves, Kenneth C. Freeman, David P. Bennett, Charles Alcock, K. H. Cook, S. L. Marshall, Dante Minniti, A. B. Tomaney, Matthew J. Lehner, Bruce A. Peterson, Christopher W. Stubbs, D. L. Welch, A. C. Becker, R. A. Allsman, William J. Sutherland, T. S. Axelrod, T. Vandehei, Peter J. Quinn, A. W. Rodgers, A. Rorabeck, Kim Griest

Publikováno v: Scopus-Elsevier

The recent suggestion that the microlensing events observed towards the Large Magellanic Cloud are due to an intervening Sgr-like dwarf galaxy is examined. A search for foreground RR Lyrae in the MACHO photometry database yields 20 stars whose distan

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e8749cebc09e68f8f0e7862da6d8efb7
https://doi.org/10.1086/311016