Zobrazeno 1 - 10
of 16
pro vyhledávání: '"A W Guzzetta"'
Autor:
Andrew W. Guzzetta, Morris J. Birnbaum, Erik Schaefer, Grace Y. Park, Kristina S. Kovacina, Sun Sik Bae, Richard A. Roth
Publikováno v:
Journal of Biological Chemistry. 278:10189-10194
Akt (also called protein kinase B) is one of the major downstream targets of the phosphatidylinositol 3-kinase pathway. This protein kinase has been implicated in insulin signaling, stimulation of cellular growth, and inhibition of apoptosis as well
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b3870d0af0143ab81b0874cff2a1eb32
https://doi.org/10.1074/jbc.m210837200
https://doi.org/10.1074/jbc.m210837200
Publikováno v:
Rapid Communications in Mass Spectrometry. 16:2067-2072
A 60 microm internal diameter (i.d.) stainless-steel needle was adapted to the orthogonal ESI probe ( microESI) of a commercial ion trap mass spectrometer, and used for capillary liquid chromatography/tandem mass spectrometry (LC/MS/MS) protein ident
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::324a2c75e459abaf78aa80c71250cb6c
https://doi.org/10.1002/rcm.829
https://doi.org/10.1002/rcm.829
Publikováno v:
Zeitschrift für Naturforschung C. 54:175-180
A purple substance (4) partially co-purified with a recombinant human B-type natriuretic peptide (hBNP), following an E. coli fermentation. The structure of the compound was elucidated by NMR, electrospray and FAB mass spectrometry. The chromophore i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::68eb3538cadb262bd0b1d15aa40ca23d
https://doi.org/10.1515/znc-1999-3-406
https://doi.org/10.1515/znc-1999-3-406
Autor:
Celine Tarnus, Rosanne Catalano, Hugues d'Orchymont, Andrew W. Guzzetta, Jean-François Navé, Jeffrey N. Higaki, Diana Hom Quon, Barbara Cordell
Publikováno v:
Journal of Biological Chemistry. 271:31885-31893
The events leading to the formation of beta-amyloid (betaA4) from its precursor (betaAPP) involve proteolytic cleavages that produce the amino and carboxyl termini of betaA4. The enzyme activities responsible for these cleavages have been termed beta
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5c2e120731ae2a8878b52ccdca6d31a6
https://doi.org/10.1074/jbc.271.50.31885
https://doi.org/10.1074/jbc.271.50.31885
Publikováno v:
Journal of Chromatography B: Biomedical Sciences and Applications. 662:335-342
Using capillary HPLC, femtomole amounts of recombinant DNA-derived human growth hormone (rhGH) have been successfully detected from solutions at nanomolar concentrations. The separation used capillaries of 15 cm × 320 μm I.D. and detection was with
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::896d9e25416ce7fb76f367164cd62e63
https://doi.org/10.1016/0378-4347(94)00312-2
https://doi.org/10.1016/0378-4347(94)00312-2
Publikováno v:
Journal of Biological Chemistry. 269:243-253
When incubated at pH 7.3, 37 degrees C, human recombinant tissue plasminogen activator accumulated 0.77 mol of isoaspartate per mol of plasminogen activator over a 14-day period. Isoaspartate was detected by enzymatic transfer of 3H-labeled methyl gr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d361dce33d1ae48444c0dda4ef25aa19
https://doi.org/10.1016/s0021-9258(17)42341-6
https://doi.org/10.1016/s0021-9258(17)42341-6
Publikováno v:
Biochemistry. 31:6339-6347
Bovine brain is known to contain two major isoforms of protein L-isoaspartyl methyltransferase (PIMT), an enzyme that facilitates repair of atypical L-isoaspartyl peptide bonds in proteins. Although the two isoforms can be separated by anion-exchange
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2f7e332000a5ac869e72206bf2f52d38
https://doi.org/10.1021/bi00142a025
https://doi.org/10.1021/bi00142a025
Autor:
William S. Hancock, John T. Stults, Thomas R. Covey, Andrew W. Guzzetta, Eleanor Canova-Davis, Victor Ling, Bori Shushan
Publikováno v:
Analytical Chemistry. 63:2909-2915
A detailed tryptic map is presented for recombinant human tissue plasminogen activator (rt-PA). Electrospray ionization mass spectrometry is utilized as an on-line HPLC detector for tryptic mapping of this glycoprotein. The additional dimension provi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c974b7abac3b2a60f2c5eca86aa3b7fc
https://doi.org/10.1021/ac00024a020
https://doi.org/10.1021/ac00024a020
Publikováno v:
Biochemistry. 30:3916-3922
High-performance cation-exchange chromatography of recombinant soluble CD4 (rCD4) allowed the resolution of four charge variants. This charge heterogeneity could be eliminated by neuraminidase treatment of rCD4 and therefore can be attributed to diff
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::014999a0eab17e336ba5494586335f97
https://doi.org/10.1021/bi00230a016
https://doi.org/10.1021/bi00230a016
Autor:
William S. Hancock, Alex Apffel, C. Souders, John Chakel, Andrew W. Guzzetta, Erno Pungor, Thabiso M'Timkulu
Publisher Summary The complexity of carbohydrate structures mandates that a variety of analytical methods be used for the study of these forms. A promising new procedure is described in this chapter that uses high-performance liquid chromatography (H
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c43797554841d64a1ef1854c13a47d6f
https://doi.org/10.1016/s0076-6879(96)71019-4
https://doi.org/10.1016/s0076-6879(96)71019-4
