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Publikováno v:
Ontogenez. 24(4)
Phenotype modulation of the human hepatoblastoma cells HepG2 treated with dimethyl sulfoxide (DMSO) was studied using indirect immunoperoxidase method with monoclonal antibodies against antigens with characteristic localization in the adult liver: cy
Autor:
D B, Staroverov, A S, Gleĭberman
Publikováno v:
Ontogenez. 24(2)
The data on distribution of alpha-fetoprotein (AFP) and gap junction protein Cx32 in mouse liver regenerating after carbon tetrachloride poisoning are presented. On the second day, when a significant number of AFP-positive cells appear in the liver,
Publikováno v:
Biulleten' eksperimental'noi biologii i meditsiny. 82(10)
Typical mature hepatocytes constituting not over several per cent of the total amount of preserved hepatocytes served as the principal site of the alpha-fetoprotein (AFP) localization in the liver of mice regenerating after the CCl4 poisoning or part
Autor:
A S, Gleĭberman
Publikováno v:
Biulleten' eksperimental'noi biologii i meditsiny. 85(5)
An acetone-formol fixation technique with subsequent paraffin-embedding suitable for immunofluorescent study of different antigens, including serum proteins, is described. This technique was used for detection of albumin, transferrin, and alpha-fetop
Autor:
A S, Gleĭberman
Publikováno v:
Biulleten' eksperimental'noi biologii i meditsiny. 82(8)
The author suggests a technique for preparation of paraffine sections from tissues fixed with acetone permitting to detect antigens of various chemical nature, including alpha-fetoprotein, antigens of mouse leukemia viruses, alcohol-soluble antigens
Autor:
L Ia, Shipova, A S, Gleĭberman
Publikováno v:
Biulleten' eksperimental'noi biologii i meditsiny. 97(3)
Highly differentiated spontaneous hepatomas of 13-15-month-old CBA mice are not capable to produce embryo-specific serum protein--alpha-fetoprotein (AFP). AFP synthesis is induced in such tumors by minimal mechanical injuries (application of mild inc
Publikováno v:
Doklady Akademii nauk SSSR. 296(5)
Autor:
A S, Gleĭberman
Publikováno v:
Biulleten' eksperimental'noi biologii i meditsiny. 93(1)
Alpha-fetoprotein (AFP) and albumin synthesis in primary hepatocyte cultures of adult mice was studied by the immunoperoxidase techniques. Albumin was detectable in all hepatocytes during the cultivation period (from day 1 to day 8). AFP could be fou
Publikováno v:
Biulleten' eksperimental'noi biologii i meditsiny. 103(4)
It has been shown by indirect immunofluorescence using monoclonal antibodies against adult rat simple epithelial prekeratins with the molecular weight of 55 kD (PK55) and 40 kD (PK40) that PK55 was expressed in the covering ectoderm until the 11th da