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pro vyhledávání: '"A O, Tsittser"'
Autor:
O. Tsittser
Publikováno v:
Nature and Society: Socio-Natural Interaction in the World-Historical Process.
Publikováno v:
Laboratornoe delo. (5)
Enzyme immunoassay was used in detection of V. cholerae cytolysin. Conjugate of immunoglobulins to purified cytolysin with horseradish peroxidase was used, obtained by the periodate technique. The method sensitivity is 2 ng/ml of purified cytolysin.
Autor:
Iu L, Kiselev, A O, Tsittser
Publikováno v:
Laboratornoe delo. (5)
The activity of purified V. cholerae cytolysin was estimated from its cytotoxic effect on L-929 cells in various growth phases. The period of target cell development was supposed to influence the sensitivity of the test. Cytolysin preparation is ther
Publikováno v:
Molekuliarnaia genetika, mikrobiologiia i virusologiia. (9)
A new method for isolation and purification of Vibrio cholerae non-01 cytolysin has been elaborated. It includes the steps of concentration by ammonium sulphate, ion exchange chromatography on DE-52-cellulose, gel filtration via ultragel AsA-44 and c