Zobrazeno 1 - 8
of 8
pro vyhledávání: '"A M, Zhelkovsky"'
Autor:
Robert J. Bauer, Larry A. McReynolds, Katharina Bilotti, Thomas C. Evans, Alexander M. Zhelkovsky, Gregory J. S. Lohman, Laura E. Crowell
Publikováno v:
PLoS ONE, Vol 12, Iss 12, p e0190062 (2017)
PLoS ONE
PLoS ONE
DNA ligases catalyze the repair of phosphate backbone breaks in DNA, acting with highest activity on breaks in one strand of duplex DNA. Some DNA ligases have also been observed to ligate two DNA fragments with short complementary overhangs or blunt-
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::578297f0e3800e4ec08e417d66e5030a
http://europepmc.org/articles/PMC5746248?pdf=render
http://europepmc.org/articles/PMC5746248?pdf=render
Publikováno v:
Journal of Biological Chemistry. 289:33608-33616
RNA and DNA ligases catalyze the formation of a phosphodiester bond between the 5'-phosphate and 3'-hydroxyl ends of nucleic acids. In this work, we describe the ability of the thermophilic RNA ligase MthRnl from Methanobacterium thermoautotrophicum
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4470471ea121798f607bff1af2ce743d
https://doi.org/10.1074/jbc.m114.612929
https://doi.org/10.1074/jbc.m114.612929
Publikováno v:
Nucleic Acids Research
Single-stranded DNA molecules (ssDNA) annealed to an RNA splint are notoriously poor substrates for DNA ligases. Herein we report the unexpectedly efficient ligation of RNA-splinted DNA by Chlorella virus DNA ligase (PBCV-1 DNA ligase). PBCV-1 DNA li
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0a3dc165353708f41daecca05ea3f2b2
https://doi.org/10.1093/nar/gkt1032
https://doi.org/10.1093/nar/gkt1032
Publikováno v:
Nucleic Acids Research
We describe a simple, specific and sensitive microRNA (miRNA) detection method that utilizes Chlorella virus DNA ligase (SplintR(®) Ligase). This two-step method involves ligation of adjacent DNA oligonucleotides hybridized to a miRNA followed by re
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3386c6c535fb73c70e90ae053ed78182
http://europepmc.org/articles/PMC5291259
http://europepmc.org/articles/PMC5291259
Publikováno v:
The Journal of biological chemistry. 270(44)
We have constructed deletions in the nonconserved regions at the amino and carboxyl ends of the poly(A) polymerase (PAP) of Saccharomyces cerevisiae and examined the effects of these truncations on function of the enzyme. PAP synthesizes a poly(A) ta
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::93e9458c79be0920638c9a32dc80b949
https://pubmed.ncbi.nlm.nih.gov/7592899
https://pubmed.ncbi.nlm.nih.gov/7592899
Publikováno v:
Nucleic Acids Research
We report a simple method of enzymatic synthesis of pre-adenylated DNA linkers/adapters for next-generation sequencing using thermostable RNA ligase from Methanobacterium thermoautotrophicum (MthRnl). Using RNA ligase for the reaction instead of the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6e6db8da476e6548ebc4467892dd1a33
http://europepmc.org/articles/PMC3177227
http://europepmc.org/articles/PMC3177227
Autor:
A D, Pivazian, H, Suzuki, A A, Vartanian, A M, Zhelkovsky, B, Farina, E, Leone, Karpeisky MYa
Publikováno v:
Biochemistry international. 9(2)
pppA2'pA2'pA appears to be a potent natural noncompetitive inhibitor of poly (ADP-ribose) transferase activity in the histone dependent reaction of ADP-ribosylation with Ki=5 microM. Moreover, it is a noncompetitive inhibitor of the Mg2+ dependent re
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::514d72a216232cb609909e59692f8ff0
https://pubmed.ncbi.nlm.nih.gov/6207831
https://pubmed.ncbi.nlm.nih.gov/6207831
Publikováno v:
BMC Molecular Biology
BMC Molecular Biology, Vol 13, Iss 1, p 24 (2012)
BMC Molecular Biology, Vol 13, Iss 1, p 24 (2012)
Background RNA ligases are essential reagents for many methods in molecular biology including NextGen RNA sequencing. To prevent ligation of RNA to itself, ATP independent mutant ligases, defective in self-adenylation, are often used in combination w
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d9c02f8ff5f61780d45097142eade1f0