Zobrazeno 1 - 10
of 12
pro vyhledávání: '"A M, Kolchinskiĭ"'
Autor:
A M, Kolchinskiĭ, D A, Griadunov, Iu P, Lysov, V M, Mikhaĭlovich, T V, Nasedkina, A Iu, Turygin, A Iu, Rubina, V E, Barskiĭ, A S, Zasedatelev
Publikováno v:
Molekuliarnaia biologiia. 38(1)
The review describes the history of creation and development of the microchip technology and its role in the human genome project in Russia. The emphasis is placed on the three-dimensional gel-based microchips developed at the Center of Biological Mi
Publikováno v:
Molekuliarnaia biologiia. 36(4)
The MAGIChip (MicroArrays of Gel-Immobilized Compounds on a chip) consists of an array of hydrophilic gel pads fixed on a hydrophobic glass surface. These pads of several picoliters to several nanoliters in volume contain the gel-immobilized nucleic
Publikováno v:
TSitologiia i genetika. 24(4)
HvRT family of repetitive DNA sequences from barley genome appears to have complex hierarchical organization. Tandem repetition of 118-bp monomers constitutes lower level of HvRT-family organization. Amplification units of the higher level consist of
Publikováno v:
Molekuliarnaia biologiia. 14(5)
We propose a simple method which allows to receive a collection of clones containing recombinant plasmids. It is based on the ligation of the longer fragment of pBR332 formed by EcoRI and BamH1 with eukaryotic DNA (from Drosophila melanogaster embryo
Publikováno v:
Molekuliarnaia biologiia. 22(1)
The organization of chromatin in D. melanogaster ribosomal repeats with and without insertions was studied. We have shown earlier that upon digestion with micrococcal nuclease a "non-transcribed" intergenic spacer produces unusual chromatin particles
Publikováno v:
Molekuliarnaia biologiia. 22(6)
Fragments of Drosophila melanogaster DNA that intensively hybridize with simple sequences poly[(dG-dT).(dC-dA)], poly[(dA).(dT)] and poly[(dG-dA).(dC-dT)] were cloned. The first two types of simple sequences are organized in these clones as separated
Publikováno v:
Molekuliarnaia biologiia. 16(2)
Cloning of fragments of ribosomal genes containing insertions in the 28S RNA gene has been reported earlier. Subcloning of DNA fragments corresponding to insertion sequences and their hybridization with DNA, RNA and polytene chromosomes from differen
Autor:
A M, Kolchinskiĭ, A D, Mirzabekov, A C, Zasedatelev, G V, Gurskiĭ, S L, Grokhovskiĭ, A L, Zhuze, B P, Gottikh
Publikováno v:
Molekuliarnaia biologiia. 9(1)
It is shown that antibiotics actinomycin D (AM), netropsin (Nt), distamycin A (DM) and the propyl analogue of distamycin A (pDM) being complexed with DNA are located within the narrow groove of DNA. A comparative investigation of the 3H-dimethyl sulp
[Length of nucleosomal DNA repeat in whole cells, fixed by freezing in the presence of formaldehyde]
Publikováno v:
Molekuliarnaia biologiia. 14(3)
It is shown that whole cells can be effectively fixed in the presence of formaldehyde at -12 degrees C. This reaction is used for the study of the native structure of chromatin. In the nuclei isolated from fixed cells the chromatin has the nucleosoma
Publikováno v:
Molekuliarnaia biologiia. 12(2)
The state of the major and the minor DNA grooves in purified mono- and oligonucleosomes and in the complexes of DNA with different histones have been studied by means of methylation of DNA with dimethyl sulphate. In nucleosomes histones shielded majo