Výsledky vyhledávání - "A C, Nicacio"

Effects of different cryopreservation methods on post-thaw culture conditions of in vitro produced bovine embryos

Autor: Mayra Elena Ortiz D'Ávila Assumpção, Renata Simões, Flavia Regina Oliveira de Barros, F. F. Paula-Lopes, A. C. Nicacio, José Antonio Visintin, Maria Angelica Peres

Publikováno v: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP

SummaryThe aim of this work was to evaluate the effect of cryopreservation protocols on subsequent development of in vitro produced bovine embryos under different culture conditions. Expanded in vitro produced blastocysts (n = 600) harvested on days

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad5d1bc59c55dc65966e1f2f6ada7235
https://doi.org/10.1017/s0967199410000717

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Immature bovine oocyte cryopreservation: Comparison of different associations with ethylene glycol, glycerol and dimethylsulfoxide

Autor: A. C. Nicacio, Renata Simões, José Antonio Visintin, Mayra Elena Ortiz D'Ávila Assumpção, Weber Beringui Feitosa, C. Yamada, Heloísa Vasconcellos Amaral Caetano

Publikováno v: Animal Reproduction Science. 99:384-388

Research on different cryoprotectants and their associations is important for successful vitrification, since greater cryoprotectant concentration of vitrification solution may be toxic to oocytes. The aim of the present research was to compare the e

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::adb339731a04b378cc4db52d7a6d1076
https://doi.org/10.1016/j.anireprosci.2006.07.001

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In vitro maturation of pig oocytes with different media, hormone and meiosis inhibitors

Autor: A. C. Nicacio, José Antonio Visintin, Mariana Groke Marques, Camilla Mota Mendes, V. P Oliveira, H.V.A. Caetano, Marco Roberto Bourg de Mello, Marcella Pecora Milazzotto, A. B. Nascimento, Mayra Elena Ortiz D'Ávila Assumpção

Publikováno v: Animal Reproduction Science. 97:375-381

This study evaluated in vitro maturation of pig oocytes in two maturation media (TCM199 and NCSU23) supplemented with 10% porcine follicular fluid (pFF) or 0.1% polyvinyl alcohol (PVA) and four hormonal treatments. The best media was then used to eva

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0f3cad5cf9802cc9334eb6fc65ab0b3d
https://doi.org/10.1016/j.anireprosci.2006.02.013

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Comparison of different methods for exogenous DNA uptake by bovine spermatozoa

Autor: Mayra Elena Ortiz D'Ávila Assumpção, José Antonio Visintin, J. S. A. Gonçalves, Mariana Groke Marques, Renata Simões, A. C. Nicacio, Weber Beringui Feitosa, Marcella Pecora Milazzotto, Flavia Regina Oliveira de Barros

Publikováno v: Brazilian Journal of Veterinary Research and Animal Science, Vol 52, Iss 1 (2015)
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP

Apesar da manipulação genética de animais domésticos ser de grande interesse para a produção animal e para a indústria farmacêutica, a sua eficiência ainda é insatisfatória. A injeção pronuclear, a técnica mais utilizada para tal propos

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fc001511e9cc618ffab5ba4d61d7fef7
http://www.revistas.usp.br/bjvras/article/view/82459

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Sperm-mediated gene transfer: effect on bovine in vitro embryo production

Autor: A. C. Nicacio, Mario Binelli, Marcella Pecora Milazzotto, Mayra Elena Ortiz D'Ávila Assumpção, F. F. Paula-Lopes, Renata Simões, José Antonio Visintin

Publikováno v: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP

SummaryThe technique of sperm-mediated gene transfer (SMGT) can be used to delivery exogenous DNA into the oocyte. However, it has low repeatability and produces inconsistent results. In order to optimize this technique, it is necessary to study the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3bf54d871bb2f712146d26819939f90a

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Maturação e desenvolvimento embrionário in vitro de oócitos bovinos após bloqueio da meiose com inibidores de MPF

Autor: A. C. Nicacio, Mariana Groke Marques, Marco Roberto Bourg de Mello, José Antonio Visintin, L. M. T. Tavares, Mayra Elena Ortiz D'Ávila Assumpção

Publikováno v: Brazilian Journal of Veterinary Research and Animal Science, Vol 48, Iss 6 (2011)
Brazilian Journal of Veterinary Research and Animal Science; Vol. 48 Núm. 6 (2011); 468-477
Brazilian Journal of Veterinary Research and Animal Science; Vol. 48 No. 6 (2011); 468-477
Brazilian Journal of Veterinary Research and Animal Science; v. 48 n. 6 (2011); 468-477
Brazilian Journal of Veterinary Research and Animal Science; V. 48 N. 6 (2011); 468-477
Brazilian Journal of Veterinary Research and Animal Science
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron:USP

O objetivo deste estudo foi avaliar a maturação e o desenvolvimento embrionário após a fecundação in vitro de oócitos bovinos que tiveram a maturação bloqueada com Butirolactona I e Roscovitina em meio de pré-maturação suplementado com so

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b276b5c62a997696854d431f659b9c11
http://www.revistas.usp.br/bjvras/article/view/34354

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INFLUÊNCIA DA TEMPERATURA DE FECUNDAÇÃO IN VITRO E DAS CÉLULAS DO Cumulus oophorus SOBRE A TAXA DE POLISPERMIA E DESENVOLVIMENTO EMBRIONÁRIO

Autor: José Antonio Visintin, Melissa Savoia Castilho Cunha, Alecsandra Sobreira de Lima, Mayra Elena Ortiz D'Ávila Assumpção, A. C. Nicacio, Mariana Groke Marques

Publikováno v: Ciência Animal Brasileira, Vol 11, Iss 2, Pp 301-306 (2010)

Effects of in vitro fertilization (IVF) temperature andcumulus oophorus cells removal after IVF or 12 h of embryoculture (IVC) on polyspermy and embryo development rates wereevaluated in swine. Oocytes and spermatozoa were incubated at37 or 38.5ºC d

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9f5e7f0ed43b0c607ead32cb3c353fc2
https://doi.org/10.5216/cab.v11i2.3402

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Vitrification with glutamine improves maturation rate of vitrified / warmed immature bovine oocytes

Autor: C, Yamada, W B, Feitosa, R, Simões, A C, Nicacio, C M, Mendes, M E O A, Assumpção, J A, Visintin

Publikováno v: Reproduction in domestic animals = Zuchthygiene. 46(1)

The current study examined the protective effects of l-glutamine and cytochalasin B during vitrification of immature bovine oocytes. Oocyte vitrification solution (PBS supplemented with 10% FCS, 25% EG, 25% DMSO and 0.5 m trehalose) was the vitrifica

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::56f51814b4e724f722f393654166af95
https://pubmed.ncbi.nlm.nih.gov/20345596

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