Zobrazeno 1 - 10
of 16
pro vyhledávání: '"A B, Fitsner"'
Autor:
N N, Sokolov, M A, El'darov, N V, Anikeĭcheva, I V, Karpychev, O T, Samko, A B, Fitsner, A A, Kalugin, E B, Khoroshutina, K G, Skriabin
Publikováno v:
Bioorganicheskaia khimiia. 18(1)
A new restriction endonuclease BbvBI free from contaminating nonspecific nucleases and phosphatases was isolated from the Bacillus brevis cells. The enzyme was purified by fractionating the sonicated cell-free extract in a two-phase PEG/dextran syste
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::5251fc1f4c265ac2bd98dac1c6996fcf
https://pubmed.ncbi.nlm.nih.gov/1326276
https://pubmed.ncbi.nlm.nih.gov/1326276
Autor:
N N, Sokolov, M A, El'darov, N V, Anikeĭcheva, I V, Karpychev, O T, Samko, A B, Fitsner, A A, Kalugin, E B, Khoroshutina, K G, Skriabin
Publikováno v:
Bioorganicheskaia khimiia. 17(9)
A new site-specific endonuclease was detected in toluene lysates of Bacillus coagulans AUCM B-732 and designated as BcoAI. The enzyme was purified by fractionation of the cell-free extract in the two-phase PEG/dextran system followed by chromatograph
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::181c765ffd7eac45b535f815ecc487de
https://pubmed.ncbi.nlm.nih.gov/1839653
https://pubmed.ncbi.nlm.nih.gov/1839653
Autor:
N N, Sokolov, Z P, Manialene, V V, Butkus, A B, Fitsner, E B, Khoroshutina, A A, Kalugin, A A, Ianulaĭtis
Publikováno v:
Bioorganicheskaia khimiia. 16(8)
New site-specific endonucleases LplI and AagI have been isolated from the Lactobacillus plantarum and Achromobacter agile cells, respectively. The enzymes' purification stages included treatment of cell-free extracts with polyethylenimine, fractionat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::a4494a4e69704b4443418256fa8a6071
https://pubmed.ncbi.nlm.nih.gov/2285421
https://pubmed.ncbi.nlm.nih.gov/2285421
Publikováno v:
Voprosy meditsinskoi khimii. 36(1)
Activity of restrictase Pae II, contrary to known restriction enzymes of the II class (except of true isoshizomere Sma), depended absolutely on monovalent cations. This pattern is untypical for restrictases of the II class. At the same time, restrict
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::8f3b2aeea1e7570cb869a4cecd73fcac
https://pubmed.ncbi.nlm.nih.gov/2111602
https://pubmed.ncbi.nlm.nih.gov/2111602
Autor:
Yu. B. Choroshoutina, O. T. Samko, I. Fodor, N. N. Sokolov, I. I. Votrin, N. V. Anikeitcheva, V. O. Kolosha, A. B. Fitsner
Publikováno v:
Molecular Biology Reports. 10:159-161
PaeI, a new restriction endonuclease from Pseudomonas aeruginosa clinical strain was isolated and characterized. It recognizes and cleaves the sequence 5'-GCATG reduced C-3' generating DNA fragments with 3'-tetranucleotide sticky ends. DNAs of pBR322
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1a3836fe8caa26c2af00fe26a92594fd
https://doi.org/10.1007/bf00778522
https://doi.org/10.1007/bf00778522
Publikováno v:
Voprosy meditsinskoi khimii. 26(4)
A new procedure is developed for isolation of highly purified preparations of restrictional endonoucleases Bam HI and Eco RI by means of fractionation with isopropyl alcohol. Restrictional endonuclease Bam HI, practically free of unspecific nucleases
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::695083ddc8244b2e057367adeadace0f
https://pubmed.ncbi.nlm.nih.gov/6256963
https://pubmed.ncbi.nlm.nih.gov/6256963
Publikováno v:
Molekuliarnaia genetika, mikrobiologiia i virusologiia. (5)
Specific endonuclease activities have been found it two Pseudomonas aeruginosa strains. Isolation and purification of enzymes and determining their specific activities have permitted one to find out that PaeI is an isoshizomer of SphI and digests the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::3af14f0155807f5497453241a2cfc494
https://pubmed.ncbi.nlm.nih.gov/3025709
https://pubmed.ncbi.nlm.nih.gov/3025709
Publikováno v:
Voprosy meditsinskoi khimii. 35(6)
Electrophoretic analysis of products obtained after hydrolysis of phage lambda DNA by means of restrictase Pae I was carried out after preincubation of DNA or the enzyme with Mg2+ as well as after preincubation of DNA simultaneously with the enzyme a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::53bc6cc67498f1031bad099df8240131
https://pubmed.ncbi.nlm.nih.gov/2629242
https://pubmed.ncbi.nlm.nih.gov/2629242
Publikováno v:
Biulleten' eksperimental'noi biologii i meditsiny. 97(2)
A rapid method for determination of restrictase Bam H1 activity in bacterial cells has been devised. It is based on cell treatment with toluene followed by incubation of toluene lysates with DNA substrate. The method is unsophisticated, well reproduc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::887a88c6d72df855a3e77713f475a174
https://pubmed.ncbi.nlm.nih.gov/6320927
https://pubmed.ncbi.nlm.nih.gov/6320927
Publikováno v:
Biulleten' eksperimental'noi biologii i meditsiny. 102(12)
5,5'-dithiobis-2-nitrobenzoic acid, N-ethylmaleimide, and parachloromercuribenzoate have been demonstrated to inhibit the activity of restrictases PaeI and PaeII from Ps. aeruginosa bacterial cells. Restrictase PaeII was more sensitive to the action
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::c2f916d9f25c91273cb32b5b8b27daf3
https://pubmed.ncbi.nlm.nih.gov/3026511
https://pubmed.ncbi.nlm.nih.gov/3026511