Výsledky vyhledávání - "A A, Laminet"

Preclinical Studies and Optimization of Specific Tumor Delivery for 741F8 sFv, an Anti-c-erbB-2 Single-Chain Species

Autor: John E. McCartney, Axel A. Laminet, James S. Huston, Louis M. Werner, Mei-Sheng Tai, Gregory P. Adams, Sen Liu, Josephine Schultz, Jamie Eisenberg, E J Wolf, Gerald Apell, L. L. Houston, Hermann Oppermann, Walter F. Stafford, Bruce J. Giantonio, Michael A. Bookman

Publikováno v: International Journal of Pharmacognosy. 33:75-91

Single-chain Fv fragments (sFv) that bind tumor-associated antigens exhibit highly specific tumor targeting characteristics, based on studies of intravenous sFv administration to immunodeficient mice bearing human tumor xenografts. These features sug

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::f14c21278870505fbb9ed0cf1aa4fcca
https://doi.org/10.3109/13880209509067090

Zobrazit plný text záznamu

Engineering disulfide-linked single-chain Fv dimers [(sFv')2] with improved solution and targeting properties: anti-digoxin 26–10 (sFv')2 and anti-c-erbB-2 741F8 (sFv')2 made by protein folding and bonded through C-terminal cysteinyl peptides

Autor: Sen Liu, Gregory P. Adams, Mei-Sheng Tai, Hermann Oppermann, Donald Jin, John E. McCartney, James S. Huston, Robert M. Hudziak, Axel A. Laminet, Irwin Fand, Michael A. Bookman, Louis M. Weiner, Walter F. Stafford, L. L. Houston

Publikováno v: "Protein Engineering, Design and Selection". 8:301-314

Single-chain Fv fusions with C-terminal cysteinyl peptides (sFv') have been engineered using model sFv proteins based upon the 26-10 anti-digoxin IgG and 741F8 anti-c-erbB-2 IgG monoclonal antibodies. As part of the 741F8 sFv construction process, th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::cbcb2dd232369614f19186a6586b3647
https://doi.org/10.1093/protein/8.3.301

Zobrazit plný text záznamu

Mammalian Cell Expression of Single–Chain Fv (sFv) Antibody Proteins and Their C–terminal Fusions with Interleukin–2 and Other Effector Domains

Autor: James S. Huston, Haimanti Dorai, Mei-Sheng Tai, Hermann Oppermann, Axel A. Laminet, John E. McCartney, L. L. Houston, Robert M. Hudziak

Publikováno v: Nature Biotechnology. 12:890-897

The production of several single-chain Fv (sFv) antibody proteins was examined by three modes of mammalian cell expression. Our primary model was the 741F8 anti-c-erbB-2 sFv, assembled as either the VH-VL or VL-VH, and expressed alone, with C-termina

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f7a79aaa738d4826579e18bc3608925b
https://doi.org/10.1038/nbt0994-890

Zobrazit plný text záznamu

Thermodynamic Partitioning Model for Hydrophobic Binding of Polypeptides by GroEL

Autor: Sabine E. Axmann, Axel A. Laminet, Andreas Plückthun, Ernst Jaeger, Karl-Peter Rücknagel, Ralph Zahn

Publikováno v: Journal of Molecular Biology. 242:150-184

From equilibrium measurements with urea we found a three-state thermodynamic and kinetic folding behavior for the precursor and mature form of Escherichia coli β-lactamase TEM2. The thermodynamic intermediate H of Escherichia coli β-lactamase and i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::78d276308632716529dc2f3c9190a123
https://doi.org/10.1006/jmbi.1994.1566

Zobrazit plný text záznamu

Affinity, specificity, and kinetics of the interaction of the SHC phosphotyrosine binding domain with asparagine-X-X-phosphotyrosine motifs of growth factor receptors

Autor: Leah Conroy, Axel A. Laminet, W. Michael Kavanaugh, Gerald Apell

Publikováno v: The Journal of biological chemistry. 271(1)

The phosphotyrosine binding (PTB) domain specifically binds to tyrosine-phosphorylated proteins, but differs in structure and mechanism of action from the SH2 domain family. We quantitated the affinity, specificity, and kinetics of the interaction of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c672358ae6769af94adbe61011c15299
https://pubmed.ncbi.nlm.nih.gov/8550571

Zobrazit plný text záznamu

Targeting c-erbB-2 expressing tumors using single-chain Fv monomers and dimers

Autor: M S, Tai, J E, McCartney, G P, Adams, D, Jin, R M, Hudziak, H, Oppermann, A A, Laminet, M A, Bookman, E J, Wolf, S, Liu

Publikováno v: Cancer research. 55

Single-chain Fv proteins containing a COOH-terminal cysteine (sFv') were constructed by using an antidigoxin 26.10 sFv and an anti-c-erbB-2 741F8 sFv. The fully active sFv' proteins were prepared by expression in Escherichia coli as insoluble inclusi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::e983a758fc8e13e9dff312237efdd7fc
https://pubmed.ncbi.nlm.nih.gov/7493381

Zobrazit plný text záznamu

Engineering disulfide-linked single-chain Fv dimers [(sFv')2] with improved solution and targeting properties: anti-digoxin 26-10 (sFv')2 and anti-c-erbB-2 741F8 (sFv')2 made by protein folding and bonded through C-terminal cysteinyl peptides

Autor: J E, McCartney, M S, Tai, R M, Hudziak, G P, Adams, L M, Weiner, D, Jin, W F, Stafford, S, Liu, M A, Bookman, A A, Laminet

Publikováno v: Protein engineering. 8(3)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::47bbe486436e833fbf852c1e8d0b85ad
https://pubmed.ncbi.nlm.nih.gov/7479692

Zobrazit plný text záznamu

Thermodynamic partitioning model for hydrophobic binding of polypeptides by GroEL. I. GroEL recognizes the signal sequences of beta-lactamase precursor

Autor: R, Zahn, S E, Axmann, K P, Rücknagel, E, Jaeger, A A, Laminet, A, Plückthun

Publikováno v: Journal of molecular biology. 242(2)

From equilibrium measurements with urea we found a three-state thermodynamic and kinetic folding behavior for the precursor and mature form of Escherichia coli beta-lactamase TEM2. The thermodynamic intermediate H of Escherichia coli beta-lactamase a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::7d6eae61038cd31a1611485348258f27
https://pubmed.ncbi.nlm.nih.gov/7916381

Zobrazit plný text záznamu

Tumor targeting in a murine tumor xenograft model with the (sFv')2 divalent form of anti-c-erbB-2 single-chain Fv

Autor: James S. Huston, Gregory P. Adams, John E. McCartney, Mei-Sheng Tai, Robert M. Hudziak, Hermann Oppermann, Walter F. Stafford, Sen Liu, Irwin Fand, Gerald Apell, Axel Laminet, Michael A. Bookman, L. L. Houston, Louis M. Weiner

Publikováno v: Cell biophysics.

This investigation has utilized novel forms of the single-chain Fv (sFv), wherein a cysteine-containing peptide has been fused to the sFv carboxyl terminus to facilitate disulfide bonding or specific cross-linking of this sFv' to make divalent (sFv')

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1182b4aa79de85594efb6e2275abef45
https://pubmed.ncbi.nlm.nih.gov/7736532

Zobrazit plný text záznamu

Folding in vitro and transport in vivo of pre-beta-lactamase are SecB independent

Autor: A A, Laminet, C A, Kumamoto, A, Plückthun

Publikováno v: Molecular microbiology. 5(1)

The rate of folding of the precursor of beta-lactamase is not influenced by the presence of SecB under conditions in which GroEL/ES retards the folding. Wild-type beta-lactamase and several mutants in the signal or the mature protein, affecting eithe

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::064ac415e26a3b074d1ece0669606d08
https://pubmed.ncbi.nlm.nih.gov/2013998

Zobrazit plný text záznamu

Vyhledávací nástroje:

Upřesnit hledání