A robust method for particulate detection of a genetic tag for 3D electron microscopy

Autor: James Rae, Charles Ferguson, Nicholas Ariotti, Richard I Webb, Han-Hao Cheng, James L Mead, James D Riches, Dominic JB Hunter, Nick Martel, Joanne Baltos, Arthur Christopoulos, Nicole S Bryce, Maria Lastra Cagigas, Sachini Fonseka, Marcel E Sayre, Edna C Hardeman, Peter W Gunning, Yann Gambin, Thomas E Hall, Robert G Parton

Publikováno v: eLife, Vol 10 (2021)

Genetic tags allow rapid localization of tagged proteins in cells and tissues. APEX, an ascorbate peroxidase, has proven to be one of the most versatile and robust genetic tags for ultrastructural localization by electron microscopy (EM). Here, we de

Externí odkaz: https://doaj.org/article/de648e408e3f416f9efd1c844760a3c8

Structure and Assembly of the PI3K-like Protein Kinases (PIKKs) Revealed by Electron Microscopy

Autor: Angel Rivera-Calzada, Andrés López-Perrote, Roberto Melero, Jasminka Boskovic, Hugo Muñoz-Hernández, Fabrizio Martino, Oscar Llorca

Publikováno v: AIMS Biophysics, Vol 2, Iss 2, Pp 36-57 (2015)

The phosphatidylinositol 3-kinase-like kinases (PIKKs) are large serine-threonine protein kinases with a catalytic domain homologous to the phosphatidylinositol 3-kinase (PI3K). All PIKK family members share a general organization comprising a conser

Externí odkaz: https://doaj.org/article/55180b8d8ddd46cea18153fcf849b73b

Structure and Assembly of the PI3K-like Protein Kinases (PIKKs) Revealed by Electron Microscopy

Autor: Andrés López-Perrote, Jasminka Boskovic, Angel Rivera-Calzada, Roberto Melero, Fabrizio Martino, Oscar Llorca, Hugo Muñoz-Hernández

Publikováno v: AIMS Biophysics, Vol 2, Iss 2, Pp 36-57 (2015)

The phosphatidylinositol 3-kinase-like kinases (PIKKs) are large serine-threonine protein kinases with a catalytic domain homologous to the phosphatidylinositol 3-kinase (PI3K). All PIKK family members share a general organization comprising a conser

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::011dda367203f3e7b923673d552d9a3b
https://doi.org/10.3934/biophy.2015.2.36

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A robust method for particulate detection of a genetic tag for 3D electron microscopy.

Autor: Rae J; The University of Queensland, Institute for Molecular Bioscience, Queensland, Australia., Ferguson C; The University of Queensland, Institute for Molecular Bioscience, Queensland, Australia., Ariotti N; Mark Wainwright Analytical Centre, University of New South Wales, Sydney, Australia.; School of Medical Sciences, University of New South Wales, Sydney, Australia., Webb RI; The University of Queensland, Centre for Microscopy and Microanalysis, Queensland, Australia., Cheng HH; The University of Queensland, Centre for Microscopy and Microanalysis, Queensland, Australia., Mead JL; The University of Queensland, Centre for Microscopy and Microanalysis, Queensland, Australia.; Division Microrobotics and Control Engineering, Department of Computing Science, University of Oldenburg, Oldenburg, Germany., Riches JD; Queensland University of Technology, Queensland, Australia., Hunter DJ; The University of Queensland, Institute for Molecular Bioscience, Queensland, Australia.; EMBL Australia Node for Single Molecule Sciences, University of New South Wales, Sydney, Australia., Martel N; The University of Queensland, Institute for Molecular Bioscience, Queensland, Australia., Baltos J; Monash Institute of Pharmaceutical Sciences, Monash University, Victoria, Australia., Christopoulos A; Monash Institute of Pharmaceutical Sciences, Monash University, Victoria, Australia., Bryce NS; School of Medical Sciences, University of New South Wales, Sydney, Australia., Cagigas ML; School of Medical Sciences, University of New South Wales, Sydney, Australia., Fonseka S; The University of Queensland, Institute for Molecular Bioscience, Queensland, Australia., Sayre ME; The University of Queensland, Centre for Microscopy and Microanalysis, Queensland, Australia., Hardeman EC; School of Medical Sciences, University of New South Wales, Sydney, Australia., Gunning PW; School of Medical Sciences, University of New South Wales, Sydney, Australia., Gambin Y; EMBL Australia Node for Single Molecule Sciences, University of New South Wales, Sydney, Australia., Hall TE; The University of Queensland, Institute for Molecular Bioscience, Queensland, Australia., Parton RG; The University of Queensland, Institute for Molecular Bioscience, Queensland, Australia.; The University of Queensland, Centre for Microscopy and Microanalysis, Queensland, Australia.

Publikováno v: ELife [Elife] 2021 Apr 27; Vol. 10. Date of Electronic Publication: 2021 Apr 27.