Stable Isotope-Resolved Metabolomics Shows Metabolic Resistance to Anti-Cancer Selenite in 3D Spheroids versus 2D Cell Cultures

Autor: Teresa W.-M. Fan, Salim S. El-Amouri, Jessica K. A. Macedo, Qing Jun Wang, Huan Song, Teresa Cassel, Andrew N. Lane

Publikováno v: Metabolites, Vol 8, Iss 3, p 40 (2018)

Conventional two-dimensional (2D) cell cultures are grown on rigid plastic substrates with unrealistic concentration gradients of O2, nutrients, and treatment agents. More importantly, 2D cultures lack cell–cell and cell–extracellular matrix (ECM

Externí odkaz: https://doaj.org/article/423d8fb11f284b058faf2cc11b54d1b1

Stable Isotope-Resolved Metabolomics Shows Metabolic Resistance to Anti-Cancer Selenite in 3D Spheroids versus 2D Cell Cultures.

Autor: Fan TW; Center for Environmental and Systems Biochemistry, Markey Cancer Center, and Depart of Toxicology and Cancer Biology, University of Kentucky, Lexington, KY 40506, USA. twmfan@gmail.com., El-Amouri SS; Center for Environmental and Systems Biochemistry, Markey Cancer Center, and Depart of Toxicology and Cancer Biology, University of Kentucky, Lexington, KY 40506, USA. sselam0@uky.edu., Macedo JKA; Center for Environmental and Systems Biochemistry, Markey Cancer Center, and Depart of Toxicology and Cancer Biology, University of Kentucky, Lexington, KY 40506, USA. jkar224@uky.edu., Wang QJ; Department of Ophthalmology and Visual Sciences, University of Kentucky, Lexington, KY 40506, USA. qingjun.wang@uky.edu., Song H; Center for Environmental and Systems Biochemistry, Markey Cancer Center, and Depart of Toxicology and Cancer Biology, University of Kentucky, Lexington, KY 40506, USA. huan.song@uky.edu., Cassel T; Center for Environmental and Systems Biochemistry, Markey Cancer Center, and Depart of Toxicology and Cancer Biology, University of Kentucky, Lexington, KY 40506, USA. terricassel@gmail.com., Lane AN; Center for Environmental and Systems Biochemistry, Markey Cancer Center, and Depart of Toxicology and Cancer Biology, University of Kentucky, Lexington, KY 40506, USA. andrew.lane@uky.edu.

Publikováno v: Metabolites [Metabolites] 2018 Jul 10; Vol. 8 (3). Date of Electronic Publication: 2018 Jul 10.