Impact of Starch Binding Domain Fusion on Activities and Starch Product Structure of 4-α-Glucanotransferase

Autor: Yu Wang, Yazhen Wu, Stefan Jarl Christensen, Štefan Janeček, Yuxiang Bai, Marie Sofie Møller, Birte Svensson

Publikováno v: Molecules, Vol 28, Iss 3, p 1320 (2023)

A broad range of enzymes are used to modify starch for various applications. Here, a thermophilic 4-α-glucanotransferase from Thermoproteus uzoniensis (TuαGT) is engineered by N-terminal fusion of the starch binding domains (SBDs) of carbohydrate b

Externí odkaz: https://doaj.org/article/c13af1bd016647a383380e891e8dd09a

In Silico Analysis of Fungal and Chloride-Dependent α-Amylases within the Family GH13 with Identification of Possible Secondary Surface-Binding Sites

Autor: Zuzana Janíčková, Štefan Janeček

Publikováno v: Molecules, Vol 26, Iss 18, p 5704 (2021)

This study brings a detailed bioinformatics analysis of fungal and chloride-dependent α-amylases from the family GH13. Overall, 268 α-amylase sequences were retrieved from subfamilies GH13_1 (39 sequences), GH13_5 (35 sequences), GH13_15 (28 sequen

Externí odkaz: https://doaj.org/article/774c5615cca04e028e0f01f0613cdcd6

How many α-amylase GH families are there in the CAZy database?

Autor: Štefan Janeček, Birte Svensson

Publikováno v: Janeček, Š & Svensson, B 2022, ' How many α-amylase GH families are there in the CAZy database? ', Amylase, vol. 6, pp. 1-10 . https://doi.org/10.1515/amylase-2022-0001

The CAZy database is a web-server for sequence-based classification of carbohydrate-active enzymes that has become the worldwide and indispensable tool for scientists engaged in this research field. It was originally created in 1991 as a classificati

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6a5987325f831f79ae42c7abb927cf51
https://doi.org/10.1515/amylase-2022-0001

TheRuminococcus bromiiamylosome protein Sas6 binds single and double helical α-glucan structures in starch

Autor: Amanda L. Photenhauer, Filipe M. Cerqueira, Rosendo Villafuerte-Vega, Krista M. Armbruster, Filip Mareček, Tiantian Chen, Zdzislaw Wawrzak, Jesse B. Hopkins, Craig W. Vander Kooi, Štefan Janeček, Brandon T. Ruotolo, Nicole M. Koropatkin

Resistant starch is a prebiotic with breakdown by gut bacteria requiring the action of specialized amylases and starch-binding proteins. The human gut symbiontRuminococcus bromiiexpresses granular starch-binding protein Sas6 (Starch Adherence System

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::ffefc67d77ebb93ba4c31d31967c74bb
https://doi.org/10.1101/2022.11.20.514607

A new GH13 subfamily represented by the α-amylase from the halophilic archaeon Haloarcula hispanica

Autor: Barbora Zámocká, Štefan Janeček

Publikováno v: Extremophiles. 24:207-217

α-Amylase catalyzes the endohydrolysis of α-1,4-glucosidic linkages in starch and related α-glucans. In the CAZy database, most α-amylases have been classified into the family GH13 counting at present more than 80,000 sequences and ~ 30 different

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::784cd220a1bf144af74ddef5afc138a4
https://doi.org/10.1007/s00792-019-01147-y