Zobrazeno 1 - 10
of 16
pro vyhledávání: '"Šárka Bidmanová"'
Publikováno v:
PLoS ONE, Vol 13, Iss 6, p e0198913 (2018)
Analytical devices that combine sensitive biological component with a physicochemical detector hold a great potential for various applications, e.g., environmental monitoring, food analysis or medical diagnostics. Continuous efforts to develop inexpe
Externí odkaz:
https://doaj.org/article/202f5caf84a0476fa083b70b859a6587
Autor:
Koen Beerens, Michaela Slanska, Mariana Amaro, David Bednar, Zbynek Prokop, Martin Hof, Šárka Bidmanová, Jan Brezovsky, Jan Sykora, Avisek Ghose, Jiri Damborsky, Piia Kokkonen
Publikováno v:
Journal of the American Chemical Society. 140:17999-18008
Enzyme engineering tends to focus on the design of active sites for the chemical steps, while the physical steps of the catalytic cycle are often overlooked. Tight binding of a substrate in an active site is beneficial for the chemical steps, whereas
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b56b6fabb0ce6ae37d75ddfb8b6248a2
https://doi.org/10.1021/jacs.8b09848
https://doi.org/10.1021/jacs.8b09848
Autor:
Zbynek Prokop, Petra Babková, Pavel Vanacek, Jan Brezovsky, Jiri Damborsky, Veronika Stepankova, Lukas Daniel, Eva Sebestova, Pavel Dvorak, Radka Chaloupková, Šárka Bidmanová
Publikováno v:
ACS Catalysis
Millions of protein sequences are being discovered at an incredible pace, representing an inexhaustible source of biocatalysts. Here, we describe an integrated system for automated in silico screening and systematic characterization of diverse family
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5e842a9ed59dcccacccb47afd9d72982
https://doi.org/10.1021/acscatal.7b03523
https://doi.org/10.1021/acscatal.7b03523
Autor:
Jiri Damborsky, Martin Trtílek, Tomas Rataj, Zbynek Prokop, Šárka Bidmanová, Marketa Kotlanova
Publikováno v:
Biosensors and Bioelectronics. 84:97-105
An advanced optical biosensor was developed based on the enzymatic reaction with halogenated aliphatic hydrocarbons that is accompanied by the fluorescence change of pH indicator. The device is applicable for the detection of halogenated contaminants
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::edbd96ff62ca8c0f296debd454f2832b
https://doi.org/10.1016/j.bios.2015.12.010
https://doi.org/10.1016/j.bios.2015.12.010
Autor:
Martin Stepan, Zbynek Prokop, Kamila Vymazalova, Michael Gruber, Šárka Bidmanová, Mark-Steven Steiner, Axel Duerkop, Otto S. Wolfbeis, Jiri Damborsky
Publikováno v:
Analytical Chemistry. 88:6044-6049
Sulfur mustard is a chemical agent of high military and terroristic significance. No effective antidote exists, and sulfur mustard can be fairly easily produced in large quantity. Rapid field testing of sulfur mustard is highly desirable. Existing an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bc691c851851619ace434457c862f088
https://doi.org/10.1021/acs.analchem.6b01272
https://doi.org/10.1021/acs.analchem.6b01272
Publikováno v:
PLoS ONE
PLoS ONE, Vol 13, Iss 6, p e0198913 (2018)
PLoS ONE, Vol 13, Iss 6, p e0198913 (2018)
Analytical devices that combine sensitive biological component with a physicochemical detector hold a great potential for various applications, e.g., environmental monitoring, food analysis or medical diagnostics. Continuous efforts to develop inexpe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1a1e5f0db91ac7e003216b4b1ee2f93d
http://europepmc.org/articles/PMC6005535
http://europepmc.org/articles/PMC6005535
Enzymes provide an exceptional combination of high catalytic activity and substrate specificity, which make them suitable biocatalysts for degradation of toxic compounds in the environment. The use of biological methods is recommended over the use of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4a0d5babac0cae06c1a2c347aedeb51f
https://doi.org/10.1039/9781788010450-00539
https://doi.org/10.1039/9781788010450-00539
Autor:
Šárka Bidmanová, Eva Hrdlickova, Zbynek Prokop, Josef Jaroš, Aleš Hampl, Jiri Damborsky, Ladislav Ilkovics
Publikováno v:
Biotechnology Journal. 9:852-860
Enzymes have a wide range of applications in different industries owing to their high specificity and efficiency. Immobilization is often used to improve biocatalyst properties, operational stability, and reusability. However, changes in the structur
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b4f05c593ffacfcbbf38e76031495042
https://doi.org/10.1002/biot.201300049
https://doi.org/10.1002/biot.201300049
Autor:
Zbynek Prokop, Radka Chaloupková, Veronika Stepankova, Tana Koudelakova, Šárka Bidmanová, Jiri Damborsky
Publikováno v:
ACS Catalysis. 3:2823-2836
One of the major barriers to the use of enzymes in industrial biotechnology is their insufficient stability under processing conditions. The use of organic solvent systems instead of aqueous media for enzymatic reactions offers numerous advantages, s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::233b179379a2793be6574bb20dc04f69
https://doi.org/10.1021/cs400684x
https://doi.org/10.1021/cs400684x
Publikováno v:
Sensors and Actuators B: Chemical. 161:93-99
An approach for the immobilization of a pH indicator in optical pH sensors and biosensors was developed. Fluorescent dyes 5(6)-carboxyfluorescein and 5(6)-carboxynaphthofluorescein were conjugated with bovine serum albumin as a non-enzymatic scaffold
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a318ada432b4282eff5d2e70d42e2f15
https://doi.org/10.1016/j.snb.2011.09.046
https://doi.org/10.1016/j.snb.2011.09.046