Low-cost stopped-flow and freeze-quench device for double mixing

Autor: Łukasz Bujnowicz, Rafał Pietras, Marcin Sarewicz, Artur Osyczka

Publikováno v: HardwareX, Vol 14, Iss , Pp e00409- (2023)

Experiments based on fast reagent mixing and observation of reaction progress are considered a powerful tool for investigating the kinetics of chemical and enzymatic reactions. Various spectroscopic methods are used in monitoring the reaction progres

Externí odkaz: https://doaj.org/article/fc5103d8c7fc4f5b91e75d0551774dfb

The High-Spin Heme bL Mutant Exposes Dominant Reaction Leading to the Formation of the Semiquinone Spin-Coupled to the [2Fe-2S]+ Cluster at the Qo Site of Rhodobacter capsulatus Cytochrome bc1

Autor: Marcin Sarewicz, Sebastian Pintscher, Łukasz Bujnowicz, Małgorzata Wolska, Artur Osyczka

Publikováno v: Frontiers in Chemistry, Vol 9 (2021)

Cytochrome bc1 (mitochondrial complex III) catalyzes electron transfer from quinols to cytochrome c and couples this reaction with proton translocation across lipid membrane; thus, it contributes to the generation of protonmotive force used for the s

Externí odkaz: https://doaj.org/article/354fbbd0b2014a55bea5fdbf3effe08d

Multichannel pulse high-current driver of magnetic actuator

Autor: Łukasz, Bujnowicz, Marcin, Sarewicz

Magnetic linear actuators have a wide range of applications. Their main advantage is the ease with which they can be controlled by regulating the current. However, high electrical power is required for obtaining a large continuous force, acceleration

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::53f2e2f57460be7441e47071b87aa1c6
https://ruj.uj.edu.pl/xmlui/handle/item/289912

Suppression of superoxide production by a spin‐spin coupling between semiquinone and the Rieske cluster in cytochrome bc 1

Autor: Artur Osyczka, Marcin Sarewicz, Patryk Kuleta, Arkadiusz Borek, Łukasz Bujnowicz

Publikováno v: FEBS Letters. 593:3-12

Catalytic reactions of quinol oxidoreductases may lead to the generation of superoxide due to electron leaks from unstable semiquinone intermediates (SQ). For cytochrome bc1 , the mechanism of suppression of superoxide generation remains unknown. We

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::973be6c26e46b90e5eea830fddcdfa83
https://doi.org/10.1002/1873-3468.13296

Catalytic Reactions and Energy Conservation in the Cytochrome bc 1 and b 6 f Complexes of Energy-Transducing Membranes

Autor: William A. Cramer, Marcin Sarewicz, Sebastian Pintscher, Artur Osyczka, Arkadiusz Borek, Rafał Pietras, Giovanni Finazzi, Łukasz Bujnowicz, Guy Hanke

Publikováno v: Chemical Reviews
Chemical Reviews, 2021, 121 (4), pp.2020-2108. ⟨10.1021/acs.chemrev.0c00712⟩
Chemical Reviews, American Chemical Society, 2021, 121 (4), pp.2020-2108. ⟨10.1021/acs.chemrev.0c00712⟩

This review focuses on key components of respiratory and photosynthetic energy-transduction systems: the cytochrome $bc_{1}$ and $b_{6}f$ ($Cytbc_{1}/b_{6}f$) membranous multisubunit homodimeric complexes. These remarkable molecular machines catalyze

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ec3e2ba23773c2b0f8268336ced785ca
https://hal.science/hal-03122713/document