A computational approach to quantifying miscounting of radiation-induced double-strand break immunofluorescent foci

Autor: Samuel P. Ingram, John-William Warmenhoven, Nicholas T. Henthorn, Amy L. Chadiwck, Elham E. Santina, Stephen J. McMahon, Jan Schuemann, Norman F. Kirkby, Ranald I. Mackay, Karen J. Kirkby, Michael J. Merchant

Publikováno v: Communications Biology, Vol 5, Iss 1, Pp 1-12 (2022)

PyFoci is a tool that simulates distributions of fluorescently labeled DNA double-strand break marker protein foci and allows the estimation of miscounting under different radiation types, doses and microscopy settings.

Externí odkaz: https://doaj.org/article/12f2d24396fc42b6b08bfd133060527c

The suitability of micronuclei as markers of relative biological effect

Autor: Charlotte J Heaven, Hannah C Wanstall, Nicholas T Henthorn, John-William Warmenhoven, Samuel P Ingram, Amy L Chadwick, Elham Santina, Jamie Honeychurch, Christine K Schmidt, Karen J Kirkby, Norman F Kirkby, Neil G Burnet, Michael J Merchant

Publikováno v: Heaven, C J, Wanstall, H C, Henthorn, N T, Warmenhoven, J-W, Ingram, S P, Chadwick, A L, Santina, E, Honeychurch, J, Schmidt, C K, Kirkby, K J, Kirkby, N F, Burnet, N G & Merchant, M J 2022, ' The suitability of micronuclei as markers of relative biological effect ', Mutagenesis, vol. 37, no. 1, pp. 3-12 . https://doi.org/10.1093/mutage/geac001
Mutagenesis

Micronucleus (MN) formation is routinely used as a biodosimeter for radiation exposures and has historically been used as a measure of DNA damage in cells. Strongly correlating with dose, MN are also suggested to indicate radiation quality, different

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b49bf05d1313e5ad799a37f6ed467a7d
https://doi.org/10.1093/mutage/geac001