Zobrazeno 241 - 250
of 297
pro vyhledávání: '"James A. Wohlschlegel"'
Autor:
Charly Chahwan, Hui Gao, John R. Yates, James A. Wohlschlegel, Victoria Martín, Veronique Blais, Paul Russell, Clare H. McGowan
Publikováno v:
The EMBO journal. 25(11)
Rad52-dependent homologous recombination (HR) is regulated by the antirecombinase activities of Srs2 and Rqh1/Sgs1 DNA helicases in fission yeast and budding yeast. Functional analysis of Srs2 in Schizosaccharomyces pombe led us to the discovery of S
Autor:
Robertus A.M. de Bruin, Paul Russell, Curt Wittenberg, Charly Chahwan, Tatyana I. Kalashnikova, James A. Wohlschlegel, John R. Yates, W. Hayes McDonald
Publikováno v:
Molecular cell. 23(4)
Summary G1-specific transcription in yeast depends upon SBF and MBF. We have identified Nrm1 ( n egative r egulator of M BF targets 1), as a stable component of MBF. NRM1 ( YNR009w ), an MBF-regulated gene expressed during late G1 phase, associates w
Publikováno v:
Analytical chemistry. 78(1)
In this paper, we present an intensity-based probability function to identify peptides from tandem mass spectra and amino acid sequence databases. The function is an approximation to the central limiting theorem, and it explicitly depends on the cumu
Autor:
Fabiola V. Rivas, Gregory J. Hannon, John R. Yates, Roy Parker, Jidong Liu, James A. Wohlschlegel
Publikováno v:
Nature cell biology. 7(12)
In animals, the majority of microRNAs regulate gene expression through the RNA interference (RNAi) machinery without inducing small-interfering RNA (siRNA)-directed mRNA cleavage1. Thus, the mechanisms by which microRNAs repress their targets have re
Autor:
Thomas F. Duchaine, Shohei Mitani, Craig C. Mello, Yanxia Bei, Sandra Harding, Gary Ruvkun, James A. Wohlschlegel, Darryl Conte, Ka Ming Pang, Daniel R. Brownell, Scott Kennedy, John R. Yates
Publikováno v:
Cell. 124(2)
SummaryIn plants, animals, and fungi, members of the Dicer family of RNase III-related enzymes process double-stranded RNA (dsRNA) to initiate small-RNA-mediated gene-silencing mechanisms. To learn how C. elegans Dicer, DCR-1, functions in multiple d
Autor:
James A. Wohlschlegel, Peter K. Kaiser
The identification of protein modification sites is an important step toward understanding the biological role of covalent modifications. For example, the mapping of phosphorylation sites and analyses of phosphorylation site mutants have tremendously
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1dcdf1df738a4354a10c08de29265c21
https://doi.org/10.1016/s0076-6879(05)99018-6
https://doi.org/10.1016/s0076-6879(05)99018-6
Publikováno v:
The Journal of biological chemistry. 279(44)
Although the modification of cellular factors by SUMO is an essential process in Saccharomyces cerevisiae, the identities of the substrates remain largely unknown. Using a mass spectrometry-based approach, we have identified 271 new SUMO targets. The
Publikováno v:
Nature methods. 1(1)
To take advantage of the potential quantitative benefits offered by tandem mass spectrometry, we have modified the method in which tandem mass spectrum data are acquired in 'shotgun' proteomic analyses. The proposed method is not data dependent and i
Autor:
W. Hayes McDonald, Chrissy Capati, John R. Yates, Karin Flick, Ikram Ouni, James A. Wohlschlegel, Peter K. Kaiser
Publikováno v:
Nature cell biology. 6(7)
The ubiquitin ligase SCF(Met30) is required for cell cycle progression in budding yeast. The critical function of SCF(Met30) is inactivation of the transcriptional activator Met4. Here we show that a single ubiquitin chain is attached to Met4 through
Development of Inhibitors That Selectively Disrupt Substrate Recognition by Cyclin-Dependent Kinases
Autor:
Anindya Dutta, James A. Wohlschlegel
Upregulation of cyclin/cdk activity and the resulting deregulation of the cell cycle is a common feature in the malignant transformation of breast epithelial cells and thus makes cyclin/cdks an attractive target for drug design. The long-term goal of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b5f11f60bba091a73f68845214429168
https://doi.org/10.21236/ada410428
https://doi.org/10.21236/ada410428