Zobrazeno 11 - 20
of 48
pro vyhledávání: '"Eric Shifrut"'
Autor:
Linda T. Vo, P. Jonathan Li, Theodore L. Roth, Murad R. Mamedov, David N. Nguyen, Peixin Amy Chen, Eric Shifrut, Jeffrey A. Bluestone, Ryan Apathy, Alexander Marson, Francis C. Szoka, Jennifer M. Puck, Daniel B. Goodman, Victoria Tobin
Publikováno v:
Nature biotechnology
Nature biotechnology, vol 38, iss 1
Nature biotechnology, vol 38, iss 1
Versatile and precise genome modifications are needed to create a wider range of adoptive cellular therapies1-5. Here we report two improvements that increase the efficiency of CRISPR-Cas9-based genome editing in clinically relevant primary cell type
Autor:
Julia Carnevale, Eric Shifrut, Nupura Kale, William A. Nyberg, Franziska Blaeschke, Yan Yi Chen, Zhongmei Li, Sagar P. Bapat, Morgan E. Diolaiti, Patrick O’Leary, Shane Vedova, Julia Belk, Bence Daniel, Theodore L. Roth, Stefanie Bachl, Alejandro Allo Anido, Brooke Prinzing, Jorge Ibañez-Vega, Shannon Lange, Dalia Haydar, Marie Luetke-Eversloh, Maelys Born-Bony, Bindu Hegde, Scott Kogan, Tobias Feuchtinger, Hideho Okada, Ansuman T. Satpathy, Kevin Shannon, Stephen Gottschalk, Justin Eyquem, Giedre Krenciute, Alan Ashworth, Alexander Marson
Publikováno v:
Nature, vol 609, iss 7925
The efficacy of adoptive T cell therapies for cancer treatment can be limited by suppressive signals from both extrinsic factors and intrinsic inhibitory checkpoints1,2. Targeted gene editing has the potential to overcome these limitations and enhanc
Autor:
Julia A. Belk, Winnie Yao, Nghi Ly, Katherine A. Freitas, Yan-Ting Chen, Quanming Shi, Alfredo M. Valencia, Eric Shifrut, Nupura Kale, Kathryn E. Yost, Connor V. Duffy, Bence Daniel, Madeline A. Hwee, Zhuang Miao, Alan Ashworth, Crystal L. Mackall, Alexander Marson, Julia Carnevale, Santosh A. Vardhana, Ansuman T. Satpathy
Publikováno v:
Cancer cell. 40(7)
T cell exhaustion limits antitumor immunity, but the molecular determinants of this process remain poorly understood. Using a chronic stimulation assay, we performed genome-wide CRISPR-Cas9 screens to systematically discover regulators of T cell exha
Autor:
Julia Carnevale, Eric Shifrut, Lisa L. Kirkemo, Alexander Marson, James Byrnes, Amy M. Weeks, James A. Wells, Alan Ashworth
Immunosuppressive factors in the tumor microenvironment (TME) impair T cell function and limit the anti-tumor immune response. T cell surface receptors that influence interactions and function in the TME are already proven targets for cancer immunoth
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0fe1295d54792502b264edd96161d8d5
https://doi.org/10.1101/2021.06.07.447379
https://doi.org/10.1101/2021.06.07.447379
Autor:
Jonathan M. Woo, Sadaf Mehdizadeh, Cody T. Mowery, Hunter M. Nisonoff, Yang Sun, Youjin V. Lee, Theodore L. Roth, George C. Hartoularos, Yutong Wang, David Lee, Eric Shifrut, Eric D. Chow, Alexander Marson, Yun S. Song, Derek Bogdanoff, Joshua Cantlon, David N. Ngyuen, James Lee, Chun Jimmie Ye
Publikováno v:
Science Advances
XYZeq is a novel scalable platform that directly encodes spatial location from tissue into single-cell RNA sequencing libraries.
Single-cell RNA sequencing (scRNA-seq) of tissues has revealed remarkable heterogeneity of cell types and states but
Single-cell RNA sequencing (scRNA-seq) of tissues has revealed remarkable heterogeneity of cell types and states but
Autor:
James R. Byrnes, Amy M. Weeks, Eric Shifrut, Julia Carnevale, Lisa Kirkemo, Alan Ashworth, Alexander Marson, James A. Wells
Publikováno v:
Molecular & Cellular Proteomics. 21:100217
Immunosuppressive factors in the tumor microenvironment (TME) impair T cell function and limit the antitumor immune response. T cell surface receptors and surface proteins that influence interactions and function in the TME are proven targets for can
Autor:
Jonathan M. Woo, Sasha Targ, Kathrin Schumann, Matthew H. Spitzer, Michelle L.T. Nguyen, Alexander Marson, Chun Jimmie Ye, Theodore L. Roth, Nikolaos Skartsis, Michael Lauber, Ruby Yu, Qizhi Tang, David N. Nguyen, Jeffrey A. Bluestone, Saskia Kolb, Eric Shifrut, Siddharth S. Raju, Jessica T. Cortez, Rachel E. Gate, Vinh Son Nguyen, Dimitre R. Simeonov
Publikováno v:
Nature immunology, vol 21, iss 11
Nature immunology
Nature immunology
Human regulatory T (Treg) cells are essential for immune homeostasis. The transcription factor FOXP3 maintains Treg cell identity, yet the complete set of key transcription factors that control Treg cell gene expression remains unknown. Here, we used
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7505bee01708a1d10ef7deae33334923
https://escholarship.org/uc/item/06z4n1xt
https://escholarship.org/uc/item/06z4n1xt
Autor:
Theodore L. Roth, Youjin V. Lee, Alexander Marson, Jeffery S. Cox, Jason A. Wojcechowskyj, Anke Meyer-Franke, David Wu, Jonathan M. Budzik, Mohamed S. Bouzidi, Vigneshwari Easwar Kumar, Krystal A. Fontaine, Weihao Zheng, Satish K. Pillai, Nevan J. Krogan, Eric Shifrut, Kelsey M. Haas, Joseph Hiatt, David E. Gordon, Ujjwal Rathore, Devin A. Cavero, Judd F. Hultquist, Eric V. Dang, Michael J. McGregor, Joel D. Ernst
Publikováno v:
Cell reports
Cell reports, vol 35, iss 6
Cell reports, vol 35, iss 6
SUMMARY Genome engineering of primary human cells with CRISPR-Cas9 has revolutionized experimental and therapeutic approaches to cell biology, but human myeloid-lineage cells have remained largely genetically intractable. We present a method for the
Autor:
Bin Zhang, Ian A. Vogel, Zhaolin Sun, Eric Shifrut, Siqi Chen, Igal Ifergan, Grace Y. Prator, Yana Zhang, Youjin V. Lee, Oren Shaked, Frédéric Van Gool, Zhongmei Li, Jessica T. Cortez, Yuanming Xu, Jeffrey A. Bluestone, Dimitre R. Simeonov, Theodore L. Roth, Elena Montauti, Deyu Fang, Y Zhang, Alexander Marson
Regulatory T cells (Tregs) are required to control immune responses and maintain homeostasis but are a significant barrier to anti-tumor immunity1. Conversely, Treg instability, characterized by loss of the master transcription factor Foxp3 and acqui
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::852ebc7166052cd61655467b8118b2f5
Autor:
Pooja Mehta, Jacob M. Luber, Anubhav N. Mathur, James A. Wells, Kelly M. Mahuron, Luv Panjabi, Joshua M. Moreau, Eric Shifrut, Margaret M. Lowe, Meromit Singer, Victoire Gouirand, Mariela L. Pauli, Jeff E. Glasgow, Ray Jupp, Devi P. Boda, Alexander Marson, Robby Grewal, Adil Daud, Michael Alvarado, Michael Rosenblum, Renny M Feldman
Publikováno v:
The Journal of Experimental Medicine
The Journal of experimental medicine, vol 217, iss 9
The Journal of experimental medicine, vol 217, iss 9
The C-type lectin layilin has been observed in several human cancers, but its function on immune cells is unknown. This study demonstrates that layilin is highly expressed on clonally expanded CD8+ T cells in human melanoma and augments integrin-medi