Zobrazeno 101 - 110
of 253
pro vyhledávání: '"Daniel I. C. Wang"'
Publikováno v:
AIChE Journal. 41:1015-1024
The formation of aggregates, rather than correctly folded polypeptide chains, is a pressing problem in biotechnology that has been difficult to approach quantitatively. The competition between folding and aggregation has been carefully analyzed for b
Autor:
Daniel I. C. Wang, Dongyang Wang, Ming Peng, Anping Guo, Haiyan Wang, Lili Chang, Xiaoping Yi, Xuchu Wang
Publikováno v:
Electrophoresis. 33(2)
Considering the importance of CBB staining in visualizing proteins in 2-DE gels, any improvement in the existing protocols with high sensitivity and good MS compatibility is of significant importance. In this study, we systematically evaluated the ef
Autor:
Daniel I. C. Wang, Liangzhi Xie
Publikováno v:
Biotechnology and Bioengineering. 43:1175-1189
In animal cell cultivation, cell density and product concentration are often low due to the accumulation of toxic end-products such as ammonia and lactate and/or the depletion of essential nutrients. A hybridoma cell line (CRL-1606) was cultivated in
Autor:
Liangzhi Xie, Daniel I. C. Wang
Publikováno v:
Biotechnology and Bioengineering. 43:1164-1174
Animal cell cultivation in vitro has been studied for more than 40 years. However, the culture medium composition has not been designed on the basis of the stoichiometric nutritional demands for animal cell growth. In this article, a model was develo
Publikováno v:
Biotechnology and Bioengineering. 43:764-771
Among the host of substratum properties that affect animal cell behavior, surface morphology has received relatively little attention. The earliest effect of surface morphology on animal cells was discovered almost a century ago when it was found tha
Autor:
Daniel I. C. Wang, Liangzhi Xie
Publikováno v:
Cytotechnology. 15:17-29
In our previous work (Xie and Wang, 1994a), a simplified stoichiometric model on energy metabolism for animal cell cultivation was developed. Fed-batch experiments were performed in T-flasks using this model in supplemental medium design (Xie and Wan
Publikováno v:
Proceedings of the 21st Annual Meeting of the European Society for Animal Cell Technology (ESACT), Dublin, Ireland, June 7-10, 2009 ISBN: 9789400708839
The Chinese Hamster Ovary (CHO) production cell line development process using methotrexate (MTX) amplification is well-studied and commonly used for biopharmaceutical processes. However, successful MTX amplification varies from clone to clone and su
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f149692ba905fd431ea817fbc4be3c1b
https://doi.org/10.1007/978-94-007-0884-6_14
https://doi.org/10.1007/978-94-007-0884-6_14
Autor:
Daniel I. C. Wang, Lam Raga A. Markely
Publikováno v:
Biotechnology progress. 28(2)
Development of recombinant Chinese Hamster Ovary (CHO) cells producing therapeutic proteins requires analyzing the quality, such as sialic acid content, of proteins produced by many cell clones. In order to perform these analyses, high-throughput met
Publikováno v:
ChemInform. 42
The novel method involves a whole cell-based monooxygenase-mediated regioselective hydroxylation and the subsequent alcohol dehydrogenase-catalyzed oxidation with cofactor recycling using acetone as the coupled substrate.
Publikováno v:
Analytical Chemistry. 65:3363-3367
Antifibronectin, monoclonal antibody was monitored through 52 h of production. Samples were automatically drawn from a bioreactor into the injection valve of an HPLC system without prior sample preparation. The hybridoma cell line was nonadherent, so