Zobrazeno 11 - 20
of 57
pro vyhledávání: '"Andrea Dlasková"'
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2310
We compared the activity of complex 1, complex 2, and the expression of the complex 1 subunit, NDUFA9, in isolated brown adipose tissue mitochondria from wild type and mitochondrial uncoupling protein 1 (UCP1) knockout mice. Direct spectrophotometric
Publikováno v:
Methods in Molecular Biology ISBN: 9781071614327
We compared the activity of complex 1, complex 2, and the expression of the complex 1 subunit, NDUFA9, in isolated brown adipose tissue mitochondria from wild type and mitochondrial uncoupling protein 1 (UCP1) knockout mice. Direct spectrophotometric
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6024fbe0319f2eaa7a1170ec0e3b371f
https://doi.org/10.1007/978-1-0716-1433-4_13
https://doi.org/10.1007/978-1-0716-1433-4_13
Autor:
Anežka Vodičková, Petr Ježek, Vojtěch Pavluch, Hana Engstová, Blanka Holendová, Jan Tauber, Andrea Dlasková, Alberto Leguina-Ruzzi
Publikováno v:
Biomolecules
Biomolecules, Vol 10, Iss 1026, p 1026 (2020)
Biomolecules; Volume 10; Issue 7; Pages: 1026
Biomolecules, Vol 10, Iss 1026, p 1026 (2020)
Biomolecules; Volume 10; Issue 7; Pages: 1026
Transcript levels for selected ATP synthase membrane FO-subunits—including DAPIT—in INS-1E cells were found to be sensitive to lowering glucose down from 11 mM, in which these cells are routinely cultured. Depending on conditions, the diminished
Publikováno v:
Scientific Reports
Scientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
Scientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
We have previously reported that transient knock-down of ATPase inhibitory factor 1 (IF1) by siRNA upregulates ATP levels and subsequently augments insulin secretion in model pancreatic β-cells INS-1E. Here we investigated how long-term IF1-overexpr
Autor:
Petr Ježek, Lukáš Alán, Nikola Capková, Hana Engstová, Vojtěch Pavluch, Frantisek Saudek, Tomáš Špaček, Andrea Dlasková, Zuzana Berková
Publikováno v:
Scientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
Scientific Reports
Scientific Reports
Hypertrophic pancreatic islets (PI) of Goto Kakizaki (GK) diabetic rats contain a lower number of β-cells vs. non-diabetic Wistar rat PI. Remaining β-cells contain reduced mitochondrial (mt) DNA per nucleus (copy number), probably due to declining
Autor:
Andrea Dlasková, Petr Ježek
Publikováno v:
Mitochondrion. 49
Type 2 diabetes progression stems from dysfunction of β-cells, besides the peripheral insulin resistance. Mitochondria as glucose sensor and regulation center are impaired at various stages of this progression. Their biogenesis and functional impair
Autor:
Lydie Plecitá-Hlavatá, Lukáš Alán, Andrea Dlasková, Jitka Špačková, Vendula Stradalova, Jan Tauber, Petr Ježek, Joerg Bewersdorf, Jan Malínský, Tomáš Špaček, Mark D. Lessard, Hana Engstová, Katarína Smolková
Publikováno v:
The FASEB Journal. 30:1941-1957
The relationship of the inner mitochondrial membrane (IMM) cristae structure and intracristal space (ICS) to oxidative phosphorylation (oxphos) is not well understood. Mitofilin (subunit Mic60) of the mitochondrial contact site and cristae organizing
Publikováno v:
Antioxidants & Redox Signaling
Aims: Pancreatic β-cell chronic lipotoxicity evolves from acute free fatty acid (FA)–mediated oxidative stress, unprotected by antioxidant mechanisms. Since mitochondrial uncoupling protein-2 (UCP2) plays antioxidant and insulin-regulating roles i
Autor:
Katarína Smolková, Andrea Dlasková, Jitka Špačková, Anežka Kahancová, Hana Engstová, Martin Bartoš, Petr Ježek, Vojtěch Pavluch, Tomáš Špaček, Lydie Hlavatá
Publikováno v:
Biochimica et biophysica acta. Bioenergetics. 1859(9)
3D super-resolution microscopy based on the direct stochastic optical reconstruction microscopy (dSTORM) with primary Alexa-Fluor-647-conjugated antibodies is a powerful method for accessing changes of objects that could be normally resolved only by
Publikováno v:
FEBS letters. 592(6)
ATPase Inhibitory factor 1 (IF1) is an endogenous regulator of mitochondrial ATP synthase, which is involved in cellular metabolism. Although great progress has been made, biological roles of IF1 and molecular mechanisms of its action are still to be