Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Rolf A. Prade"'
Autor:
César Rafael Fanchini Terrasan, Mariane Paludetti Zubieta, Everton P. Antoniel, Rolf A. Prade, Marcelo Ventura Rubio, Jaqueline Aline Gerhardt, Gabriela F. Persinoti, André Damasio, Fabiano Jares Contesini
Publikováno v:
Microbial Biotechnology, Vol 13, Iss 4, Pp 1245-1253 (2020)
Microbial Biotechnology
Microbial Biotechnology
Summary Filamentous fungi are important cell factories for large‐scale enzyme production. However, production levels are often low, and this limitation has stimulated research focusing on the manipulation of genes with predicted function in the pro
Publikováno v:
Microbial Cell Factories, Vol 18, Iss 1, Pp 1-12 (2019)
Microbial Cell Factories
Microbial Cell Factories
BackgroundBiomass contains cellulose (C6-sugars), hemicellulose (C5-sugars) and lignin. Biomass ranks amongst the most abundant hydrocarbon resources on earth. However, biomass is recalcitrant to enzymatic digestion by cellulases. Physicochemical pre
Publikováno v:
Bioresource Technology. 188:219-225
A trickle bed reactor (TBR) with recycle was designed and tested using Aspergillus nidulans with a pyridoxine marker and over-expressing/secreting recombinant client xylanase B (XynB). The pyridoxine marker prevented the fungus from synthesizing its
Autor:
Fernando Segato, Maria de Lourdes Teixeira de Moraes Polizeli, Fausto Almeida, Fabio M. Squina, André Damasio, Tony Marcio da Silva, Héctor Francisco Terenzi, Rolf A. Prade, Adriana Franco Paes Leme, João Atílio Jorge, Daniela Ribeiro
Publikováno v:
Process Biochemistry. 46:1236-1242
A xylanase was cloned from Aspergillus niveus and successfully expressed in Aspergillus nidulans (XAN). The full-length gene consisted of 890 bp and encoded 275 mature amino acids with a calculated mass of 31.3 kDa. The deduced amino acid sequence wa
Publikováno v:
Protein Expression and Purification. 68:65-71
Agricultural and forest waste products are abundant and low-cost biomass sources useful in renewable fuel energy and feedstock preparation. Hydrolysis of a major biomass component, hemicellulose, is accomplished by the action of endo-xylanases. React
Publikováno v:
Microbiology and molecular biology reviews : MMBR. 78(4)
SUMMARY Biomass is constructed of dense recalcitrant polymeric materials: proteins, lignin, and holocellulose, a fraction constituting fibrous cellulose wrapped in hemicellulose-pectin. Bacteria and fungi are abundant in soil and forest floors, activ
Publikováno v:
Journal of industrial microbiologybiotechnology. 41(10)
The present study investigated the limitation of pyridoxine on an Aspergillus nidulans culture that produces xylanase B (XynB) as a client enzyme and was unable to synthesize pyridoxine. This technique was used to limit cell growth and divert substra
Autor:
Rebecca C. Gregory, André Damasio, Liliane Fraga Costa Ribeiro, Richard J. Ward, João Atílio Jorge, Marcelo Ventura Rubio, Gabriela Leal Vitcosque, Maria de Lourdes Teixeira de Moraes Polizeli, Paul H. Walton, Lucas Ferreira Ribeiro, Rolf A. Prade, Marcos Silveira Buckeridge, Fabio M. Squina, Rosymar Coutinho de Lucas
Publikováno v:
Biotechnology for Biofuels
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
Background The search for novel thermostable xylanases for industrial use has intensified in recent years, and thermophilic fungi are a promising source of useful enzymes. The present work reports the heterologous expression and biochemical character
Autor:
Lívia B. Brenelli, André Damasio, Juliano Bragatto, Rolf A. Prade, Mário T. Murakami, Thiago Augusto Gonçalves, Fabio M. Squina, Thabata M. Alvarez, Roberto Ruller, Ana Paula Citadini, A.F. Paes Leme, Fernando Segato
Publikováno v:
Bioresource technology. 119
Plant cell wall degrading enzymes are key technological components in biomass bioconversion platforms for lignocellulosic materials transformation. Cost effective production of enzymes and identification of efficient degradation routes are two econom
Autor:
Rolf A. Prade, Adriane M. F. Milagres
Publikováno v:
Enzyme and Microbial Technology. 16:627-632
An isolate of the fungus Penicillium janthinellum that produces large amounts of xylanase with no detectable cellulase activity was used as a model system to study enzyme induction and production and to illustrate the use of such preparations in the