Zobrazeno 1 - 10
of 22
pro vyhledávání: '"Albert E. Dahlberg"'
Autor:
Hasan DeMirci, Steven T. Gregory, Jennifer F. Carr, Albert E. Dahlberg, Eileen L. Murphy, Gerwald Jogl, Leyi Wang, Frank V. Murphy, Scott C. Blanchard
Publikováno v:
RNA. 19:1791-1801
The ribosome decodes mRNA by monitoring the geometry of codon–anticodon base-pairing using a set of universally conserved 16S rRNA nucleotides within the conformationally dynamic decoding site. By applying single-molecule FRET and X-ray crystallogr
Publikováno v:
RNA. 10:28-33
A mechanistic understanding of ribosome function demands knowledge of the conformational changes that occur during protein synthesis. One current model proposes a conformational switch in Helix 27 (H27) of 16S rRNA involved in the decoding of mRNA. T
Publikováno v:
Journal of Molecular Biology. 309:333-338
We have isolated spontaneous streptomycin-resistant, streptomycin-dependent and streptomycin-pseudo-dependent mutants of the thermophilic bacterium Thermus thermophilus IB-21. All mutant phenotypes were found to result from single amino acid substitu
Publikováno v:
Journal of Molecular Biology. 293:1-8
Three ribosomal RNA mutations conferring resistance to the antibiotic kasugamycin were isolated using a strain of Escherichia coli in which all of the rRNA is transcribed from a plasmid-encoded rrn operon. The mutations, A794G, G926A, and A1519C, map
Autor:
Steven T. Gregory, Albert E. Dahlberg
Publikováno v:
Journal of Molecular Biology. 285:1475-1483
Evidence is presented for the participation of the P loop (nucleotides G2250-C2254) of 23 S rRNA in establishing the tertiary structure of the peptidyl transferase center. Single base substitutions were introduced into the P loop, which participates
Publikováno v:
Journal of Biological Chemistry. 271:4693-4698
We have investigated the highly conserved GAUCA sequence of small subunit ribosomal RNA. Within this region, the invariant nucleotides G1530 and A1531 of Escherichia coli 16 S rRNA were mutagenized to A1530/G1531. These base changes caused a lethal p
Autor:
Albert E. Dahlberg, Michael O'Connor
Publikováno v:
Journal of Molecular Biology. 254:838-847
The role of ribosomal RNA in maintaining the accuracy of translation has been investigated genetically by selecting for rRNA mutations that promoted frameshifting at a specific site in a reporter gene in Escherichia coli. Mutations were recovered in
Autor:
Eileen L. Murphy, Frank V. Murphy, Albert E. Dahlberg, Steven T. Gregory, Gerwald Jogl, Hasan DeMirci
Publikováno v:
Nature communications
During protein synthesis, the ribosome selects aminoacyl-transfer RNAs with anticodons matching the messenger RNA codon present in the A site of the small ribosomal subunit. The aminoglycoside antibiotic streptomycin disrupts decoding by binding clos
Autor:
Albert E. Dahlberg, Kathy R. Lieberman
Publikováno v:
Journal of Biological Chemistry. 269:16163-16169
We have constructed the double mutant G2252C/G2253C in Escherichia coli 23 S rRNA by site-directed mutagenesis. These phylogenetically conserved residues are protected from chemical modification by the 3' CCA terminus of the peptidyl-tRNA site (P sit
Publikováno v:
Nucleic Acids Research. 20:4221-4227
A series of base substitution and deletion mutations were constructed in the highly conserved 530 stem and loop region of E. coli 16S rRNA involved in binding of tRNA to the ribosomal A site. Base substitution and deletion of G517 produced significan