Zobrazeno 1 - 10
of 18
pro vyhledávání: '"Wayne B. Anderson"'
Publikováno v:
Archives of Biochemistry and Biophysics. 363:121-128
In fibroblasts, the protein kinase C (PKC) activator phorbol 12-myristate 13-acetate (PMA) stimulates phospholipase D (PLD)-mediated hydrolysis of both phosphatidylcholine (PtdCho) and phosphatidylethanolamine (PtdEtn) by PKC-alpha-mediated nonphosph
Autor:
Alma F. Ferrier, Douglas R. Lowy, Giovanna Benvenuto, Deborah K. Morrison, Jeffrey E. DeClue, Wayne B. Anderson, Michael Lee
Publikováno v:
Journal of Biological Chemistry. 272:2136-2142
The Raf-1 serine/threonine protein kinase plays a central role in many of the mitogenic signaling pathways regulating cell growth and differentiation. The regulation of Raf-1 is complex, and involves protein-protein interactions as well as changes in
Publikováno v:
Biochemical and Biophysical Research Communications. 223:98-103
Subcellular redistribution (translocation) was initiated by treatment of NIH 3T3 cells overexpressing different epitope-tagged fragments of PKC epsilon with PMA, and was analyzed by immunocytochemistry. The PMA-induced translocation of holo PKC epsil
Publikováno v:
Biochemical Journal. 302:649-654
The phospholipase D (PLD)-mediated synthesis of phosphatidylethanol (PtdEtOH) and the hydrolysis of phosphatidylethanolamine (PtdEtn) and phosphatidylcholine (PtdCho) were examined in drug-sensitive and multidrug-resistant lines of MCF-7 human breast
Publikováno v:
Journal of Biological Chemistry. 269:4761-4766
To examine the biological properties of protein kinase C (PKC)-delta and -epsilon NIH 3T3 cells were stably transfected with metallothionein-based expression vectors that overexpressed these isoforms. In addition to their inducibility by Zn2+, the pr
Publikováno v:
The Journal of biological chemistry. 279(47)
Recently we reported that simultaneous treatment of NIH 3T3 cells with the combination of phorbol myristate acetate (PMA) and hydrogen peroxide (H2O2) resulted in synergistic activation of Raf-1 kinase (Lee, M., Petrovics, G., and Anderson, W. B. (20
Publikováno v:
Biochemical and biophysical research communications. 311(4)
We have previously demonstrated that a 33kDa C-terminal fragment of c-Raf-1 underwent a mobility shift in response to hydrogen peroxide (H(2)O(2)) and phorbol myristate acetate (PMA), respectively. In this study, we have demonstrated that H(2)O(2) in
Publikováno v:
Archives of biochemistry and biophysics. 397(2)
Phorbol 12-myristate-13-acetate (PMA), a potent tumor promoter and activator of most protein kinase C (PKC) isotypes, was found to significantly inhibit the growth of low population density (1-5% confluency) NIH 3T3 cells. Higher cell population dens
Autor:
Maribeth V. Eiden, Wayne B. Anderson, Betsy D. Leverett, Gyorgy Petrovics, Zoltán Oláh, Zsolt Jobbagy, Nicholas M. Dean
Publikováno v:
The Journal of biological chemistry. 274(11)
The membrane receptors for the gibbon ape leukemia retrovirus and the amphotropic murine retrovirus serve normal cellular functions as sodium-dependent phosphate transporters (Pit-1 and Pit-2, respectively). Our earlier studies established that activ
Publikováno v:
Biochemical and biophysical research communications. 214(2)
Experiments were carried out to determine Raf-1 protein kinase domain fragments which exhibit a characteristic electrophoretic mobility shift noted with Raf-1 protein kinase in response to serum and phorbol ester (PMA) treatment of serum-deprived NIH