Zobrazeno 1 - 10
of 14
pro vyhledávání: 'Tuček, Jan'
Autor:
Stanislav Tuček, Jan Jakubík
Publikováno v:
Scopus-Elsevier
We have found earlier that the neuromuscular blocker alcuronium binds to cardiac muscarinic receptors simultaneously with their specific antagonist [3H]methyl-N-scopolamine ([3H]NMS) and allosterically increases their affinity to this ligand. Nothing
Publikováno v:
Naunyn-Schmiedeberg's archives of pharmacology. 368(5)
Glucocorticoids affect the expression and density of neurotransmitter receptors in many tissues but data concerning the heart are contradictory and incomplete. We injected rats with hydrocortisone for 1-12 days and measured the densities of cardiac m
Autor:
Jan Proška, Stanislav Tuček
Publikováno v:
European journal of pharmacology. 305(1-3)
It was discovered recently that alcuronium and strychnine (which is a precursor of alcuronium) allosterically increase the affinity of cardiac muscarinic receptors for the antagonist, N-methylscopolamine. We have now investigated the effects of l-ebu
Autor:
Stanislav Tuček, Jan Jakubík
Publikováno v:
European journal of pharmacology. 289(2)
Changes in the allosteric effects of alcuronium on rat cardiac muscarinic receptors were investigated after chemical modifications of S-S bonds or free carboxyl groups. In membranes pretreated with dithiothreitol, alcuronium lost its positive action
Autor:
Jan Jakubík, Stanislav Tuček
1 By measuring the binding of N-[3H-methyl]-scopolamine ([3H]-NMS) and of unlabelled subtype-specific muscarinic antagonists, two populations of muscarinic binding sites can be distinguished in the membranes of cardiac ventricles taken from 1-day-old
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::61d2c72a2d01cb31fd244dd65b779fee
https://europepmc.org/articles/PMC1510540/
https://europepmc.org/articles/PMC1510540/
Publikováno v:
Journal of neuroscience methods. 41(1)
A method is described for reversed-phase HPLC separation of acetylcholine and choline and of their homologues in tissue extracts or perfusion fluids, combined with postcolumn enzymatic derivatization and fluorometric quantification. The separation oc
Autor:
Jan R̀íčíný, Stanislav Tuček
Publikováno v:
Journal of Neurochemistry. 39:668-673
The effects of (-)-hydroxycitrate (OHC) and citrate on the concentration of acetylcoenzyme A (acetyl-CoA) and acetylcholine (ACh) in the tissue and on the release of ACh into the medium were investigated in experiments on slices of rat caudate nuclei
Publikováno v:
FEBS Letters. (2):275-279
We investigated whether muscarinic receptors of the M1–M4 receptor subtypes are constitutively active. We have found that the synthesis of cyclic AMP was enhanced by the muscarinic antagonists atropine and N-methylscopolamine (NMS) in Chinese hamst
Autor:
Randáková, Alena, Nelic, Dominik, Ungerová, Dana, Nwokoye, Peter, Su, Qiwen, Doležal, Vladimír, El‐Fakahany, Esam E., Boulos, John, Jakubík, Jan, El-Fakahany, Esam E
Publikováno v:
British Journal of Pharmacology; May2020, Vol. 177 Issue 9, p2073-2089, 17p, 2 Color Photographs, 1 Chart, 8 Graphs
Autor:
Stanislav Tuček, Jan Říčný
Publikováno v:
Analytical biochemistry. 103(2)
The radioenzymatic method of acetyl coenzyme A (acetyl-CoA) determination in the brain devised by Shea and Aprison (1977, J. Neurochem. 28 , 51–58.) is based on the principle that tissue acetyl-CoA is incubated with [ 14 C]choline and choline acety