Zobrazeno 1 - 10
of 11
pro vyhledávání: '"Andrea Dlasková"'
Autor:
Jan Ježek, Blanka Holendová, Tomáš Špaček, Lydie Plecitá-Hlavatá, Jan Tauber, Klára Gotvaldová, Vladimír Křen, Lucie Petrásková, Hana Engstová, Andrea Dlasková, Petr Ježek, Katarína Smolková, Jitka Špačková
Publikováno v:
Antioxidants & Redox Signaling
Aims: Glucose-stimulated insulin secretion (GSIS) in pancreatic β cells was expected to enhance mitochondrial superoxide formation. Hence, we elucidated relevant redox equilibria. Results: Unexpectedly, INS-1E cells at transitions from 3 (11 mM; pan
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2310
We compared the activity of complex 1, complex 2, and the expression of the complex 1 subunit, NDUFA9, in isolated brown adipose tissue mitochondria from wild type and mitochondrial uncoupling protein 1 (UCP1) knockout mice. Direct spectrophotometric
Autor:
Anežka Vodičková, Petr Ježek, Vojtěch Pavluch, Hana Engstová, Blanka Holendová, Jan Tauber, Andrea Dlasková, Alberto Leguina-Ruzzi
Publikováno v:
Biomolecules
Biomolecules, Vol 10, Iss 1026, p 1026 (2020)
Biomolecules; Volume 10; Issue 7; Pages: 1026
Biomolecules, Vol 10, Iss 1026, p 1026 (2020)
Biomolecules; Volume 10; Issue 7; Pages: 1026
Transcript levels for selected ATP synthase membrane FO-subunits—including DAPIT—in INS-1E cells were found to be sensitive to lowering glucose down from 11 mM, in which these cells are routinely cultured. Depending on conditions, the diminished
Publikováno v:
Scientific Reports
Scientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
Scientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
We have previously reported that transient knock-down of ATPase inhibitory factor 1 (IF1) by siRNA upregulates ATP levels and subsequently augments insulin secretion in model pancreatic β-cells INS-1E. Here we investigated how long-term IF1-overexpr
Autor:
Petr Ježek, Lukáš Alán, Nikola Capková, Hana Engstová, Vojtěch Pavluch, Frantisek Saudek, Tomáš Špaček, Andrea Dlasková, Zuzana Berková
Publikováno v:
Scientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
Scientific Reports
Scientific Reports
Hypertrophic pancreatic islets (PI) of Goto Kakizaki (GK) diabetic rats contain a lower number of β-cells vs. non-diabetic Wistar rat PI. Remaining β-cells contain reduced mitochondrial (mt) DNA per nucleus (copy number), probably due to declining
Publikováno v:
Antioxidants & Redox Signaling
Aims: Pancreatic β-cell chronic lipotoxicity evolves from acute free fatty acid (FA)–mediated oxidative stress, unprotected by antioxidant mechanisms. Since mitochondrial uncoupling protein-2 (UCP2) plays antioxidant and insulin-regulating roles i
Autor:
Katarína Smolková, Andrea Dlasková, Jitka Špačková, Anežka Kahancová, Hana Engstová, Martin Bartoš, Petr Ježek, Vojtěch Pavluch, Tomáš Špaček, Lydie Hlavatá
Publikováno v:
Biochimica et biophysica acta. Bioenergetics. 1859(9)
3D super-resolution microscopy based on the direct stochastic optical reconstruction microscopy (dSTORM) with primary Alexa-Fluor-647-conjugated antibodies is a powerful method for accessing changes of objects that could be normally resolved only by
Publikováno v:
FEBS letters. 592(6)
ATPase Inhibitory factor 1 (IF1) is an endogenous regulator of mitochondrial ATP synthase, which is involved in cellular metabolism. Although great progress has been made, biological roles of IF1 and molecular mechanisms of its action are still to be
Autor:
Jitka Šantorová, Andrea Dlasková, Zuzana Berková, Petr Ježek, Joerg Bewersdorf, Lydie Plecitá-Hlavatá, Frantisek Saudek, Tomáš Špaček, Mark D. Lessard
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Bioenergetics. 1797:1327-1341
Insulin production in pancreatic beta-cells is critically linked to mitochondrial oxidative phosphorylation. Increased ATP production triggered by blood glucose represents the beta-cells' glucose sensor. Type-2 diabetes mellitus results from insulin
Autor:
Martin Modrianský, Andrea Dlasková, Eva Škobisová, Markéta Žáčková, Katarína Smolková, Tomáš Špaček, Petr Ježek, Michal Růžička, Jitka Šantorová
Publikováno v:
The International Journal of Biochemistry & Cell Biology. 37:809-821
Rat liver mitochondria contain a negligible amount of mitochondrial uncoupling protein UCP2 as indicated by 3H-GTP binding. UCP2 recruitment in hepatocytes during infection may serve to decrease mitochondrial production of reactive oxygen species (RO