Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Simon Peter Williams"'
Autor:
Peter M. Haggie, Alexandra M. Fulton, Stanley W. Botchway, Rebecca Harmston, Randolf Jordan, Anthony W. Parker, Ignasi Barba, Kevin M. Brindle, Simon-Peter Williams
Publikováno v:
Biochemical Journal. 390:787-790
A novel method for the fluorescence detection of proteins in cells is described in the present study. Proteins are labelled by the selective biosynthetic incorporation of 5-hydroxytryptophan and the label is detected via selective two-photon excitati
Publikováno v:
Biophysical Journal. 72:490-498
Three glycolytic enzymes, hexokinase, phosphoglycerate kinase, and pyruvate kinase, were fluorine labeled in the yeast Saccharomyces cerevisiae by biosynthetic incorporation of 5-fluorotryptophan. 19F NMR longitudinal relaxation time measurements on
Publikováno v:
Biochemical Society Transactions. 23:376-381
Publikováno v:
Journal of Molecular Recognition. 6:159-165
Models of metabolic flux regulation are frequently based on an extrapolation of the kinetic properties of enzymes measured in vitro to the intact cell. Such an extrapolation assumes a detailed knowledge of the intracellular environment of these enzym
Publikováno v:
Biochemistry. 32:4895-4902
Yeast phosphoglycerate kinase was selectively fluorine-labeled in vivo by inducing enzyme synthesis in stationary phase cells in the presence of 5-fluorotryptophan. Inducible expression was obtained using a galactose-inducible expression vector conta
Publikováno v:
Biochemical Society Transactions. 19:997-1001
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America. 93(13)
31P NMR magnetization transfer measurements have been used to measure the steady state flux between Pi and ATP in yeast cells genetically modified to overexpress an adenine nucleotide translocase isoform. An increase in Pi -> ATP flux and apparent ra
Publikováno v:
FEBS Letters. (1):121-124
Phosphoglycerate kinase was fluorine-labelled in the yeast Saccharomyces cerevisiae by inducing enzyme synthesis in the presence of 5-fluorotryptophan. 19F NMR measurements on the intact cells showed two resonances from the two tryptophan residues in