Zobrazeno 1 - 10
of 168
pro vyhledávání: '"Hans Clevers"'
Publikováno v:
eLife, Vol 12 (2023)
Lung cancer (LC) is the leading cause of cancer-related deaths worldwide. Traditional therapeutic approaches such as chemotherapy or radiotherapy have provided only a marginal improvement in the treatment of lung carcinomas. Inhibitors targeting spec
Externí odkaz:
https://doaj.org/article/a3265402411444aea5ccb08039258e35
Autor:
Adriana Martinez-Silgado, Fjodor A. Yousef Yengej, Jens Puschhof, Veerle Geurts, Charelle Boot, Maarten H. Geurts, Maarten B. Rookmaaker, Marianne C. Verhaar, Joep Beumer, Hans Clevers
Publikováno v:
STAR Protocols, Vol 3, Iss 3, Pp 101639- (2022)
Summary: Intestinal organoids are three-dimensional cultures that resemble key aspects of the epithelium of origin. Here, we describe how to differentiate human small intestinal organoids by combining growth media variations and genetic engineering.
Externí odkaz:
https://doaj.org/article/dcf1297473074b90b8864176f730bcd6
Autor:
Else Driehuis, Ana Gracanin, Robert Gerhardus Jacob Vries, Hans Clevers, Sylvia Fernández Boj
Publikováno v:
STAR Protocols, Vol 1, Iss 3, Pp 100192- (2020)
Summary: Establishment of patient-derived adult stem cell-based pancreatic (tumor) organoids was first described in 2015. Since then, multiple laboratories have demonstrated the robustness of this method. We recently described the generation of a pan
Externí odkaz:
https://doaj.org/article/67b7e2bf8e464e5db794ff834d353b6b
Autor:
Chris Jenske de Witte, Jose Espejo Valle-Inclan, Nizar Hami, Kadi Lõhmussaar, Oded Kopper, Celien Philomena Henrieke Vreuls, Geertruida Nellie Jonges, Paul van Diest, Luan Nguyen, Hans Clevers, Wigard Pieter Kloosterman, Edwin Cuppen, Hugo Johannes Gerhardus Snippert, Ronald Peter Zweemer, Petronella Oda Witteveen, Ellen Stelloo
Publikováno v:
Cell Reports, Vol 31, Iss 11, Pp 107762- (2020)
Summary: There remains an unmet need for preclinical models to enable personalized therapy for ovarian cancer (OC) patients. Here we evaluate the capacity of patient-derived organoids (PDOs) to predict clinical drug response and functional consequenc
Externí odkaz:
https://doaj.org/article/710cd95200e946f6b51756da239c114a
Autor:
Krijn K. Dijkstra, Kim Monkhorst, Luuk J. Schipper, Koen J. Hartemink, Egbert F. Smit, Sovann Kaing, Rosa de Groot, Monika C. Wolkers, Hans Clevers, Edwin Cuppen, Emile E. Voest
Publikováno v:
Cell Reports, Vol 31, Iss 5, Pp - (2020)
Summary: Clinical implementation of tumor organoids for personalized medicine requires that pure tumor organoids can be reliably established. Here, we present our experience with organoid cultures from >70 non-small cell lung cancer (NSCLC) samples.
Externí odkaz:
https://doaj.org/article/b469827b6c1c427c86f583c772baa165
Autor:
Fjodor A. Yousef Yengej, Jitske Jansen, Maarten B. Rookmaaker, Marianne C. Verhaar, Hans Clevers
Publikováno v:
Cells, Vol 9, Iss 6, p 1326 (2020)
In the past five years, pluripotent stem cell (PSC)-derived kidney organoids and adult stem or progenitor cell (ASC)-based kidney tubuloids have emerged as advanced in vitro models of kidney development, physiology, and disease. PSC-derived organoids
Externí odkaz:
https://doaj.org/article/050a4c9108de4b7989adce557c0ae243
Autor:
Hedwig S. Kruitwagen, Loes A. Oosterhoff, Monique E. van Wolferen, Chen Chen, Sathidpak Nantasanti Assawarachan, Kerstin Schneeberger, Anne Kummeling, Giora van Straten, Ies C. Akkerdaas, Christel R. Vinke, Frank G. van Steenbeek, Leonie W.L. van Bruggen, Jeannette Wolfswinkel, Guy C.M. Grinwis, Sabine A. Fuchs, Helmuth Gehart, Niels Geijsen, Robert G. Vries, Hans Clevers, Jan Rothuizen, Baukje A. Schotanus, Louis C. Penning, Bart Spee
Publikováno v:
Cells, Vol 9, Iss 2, p 410 (2020)
The shortage of liver organ donors is increasing and the need for viable alternatives is urgent. Liver cell (hepatocyte) transplantation may be a less invasive treatment compared with liver transplantation. Unfortunately, hepatocytes cannot be expand
Externí odkaz:
https://doaj.org/article/c674d01025924804a5b649c947eca488
Autor:
Else Driehuis, Sacha Spelier, Irati Beltrán Hernández, Remco de Bree, Stefan M. Willems, Hans Clevers, Sabrina Oliveira
Publikováno v:
Journal of Clinical Medicine, Vol 8, Iss 11, p 1880 (2019)
Patients diagnosed with head and neck squamous cell carcinoma (HNSCC) are currently treated with surgery and/or radio- and chemotherapy. Despite these therapeutic interventions, 40% of patients relapse, urging the need for more effective therapies. I
Externí odkaz:
https://doaj.org/article/afe3a5224f64451fb7ffbf1bbfaa93ab
Autor:
Carla S Verissimo, René M Overmeer, Bas Ponsioen, Jarno Drost, Sander Mertens, Ingrid Verlaan-Klink, Bastiaan van Gerwen, Marieke van der Ven, Marc van de Wetering, David A Egan, René Bernards, Hans Clevers, Johannes L Bos, Hugo J Snippert
Publikováno v:
eLife, Vol 5 (2016)
Colorectal cancer (CRC) organoids can be derived from almost all CRC patients and therefore capture the genetic diversity of this disease. We assembled a panel of CRC organoids carrying either wild-type or mutant RAS, as well as normal organoids and
Externí odkaz:
https://doaj.org/article/7501d8e3fc1b4a42aa7676d2bb0a2f09
Autor:
Flavio Bonanini, Dorota Kurek, Sara Previdi, Arnaud Nicolas, Delilah Hendriks, Sander de Ruiter, Marine Meyer, Maria Clapés Cabrer, Roelof Dinkelberg, Silvia Bonilla García, Bart Kramer, Thomas Olivier, Huili Hu, Carmen López-Iglesias, Frederik Schavemaker, Erik Walinga, Devanjali Dutta, Karla Queiroz, Karel Domansky, Bob Ronden, Jos Joore, Henriette L. Lanz, Peter J. Peters, Sebastiaan J. Trietsch, Hans Clevers, Paul Vulto
Publikováno v:
Angiogenesis
Angiogenesis, 25(4), 455-470. Springer, Cham
Angiogenesis, 25(4), 455-470. Springer Netherlands
Angiogenesis, 25(4), 455-470. Springer, Cham
Angiogenesis, 25(4), 455-470. Springer Netherlands
With recent progress in modeling liver organogenesis and regeneration, the lack of vasculature is becoming the bottleneck in progressing our ability to model human hepatic tissues in vitro. Here, we introduce a platform for routine grafting of liver