Zobrazeno 1 - 10
of 14
pro vyhledávání: '"Kenneth J. Livak"'
Publikováno v:
Biotechnology Journal. 2:33-35
The small size of miRNAs makes profiling of all the 462 known human miRNAs difficult using single cell samples. Recently, we showed that judicious sequence partitioning between RT primers and second strand synthesis primers permitted multiplexed RT-P
Publikováno v:
Proceedings of the National Academy of Sciences. 92:427-431
The dopamine D4 receptor is one of five receptors known to function in mammalian dopaminergic pathways. The DNA sequence of the human dopamine D4 receptor gene (DRD4) has previously been investigated in several populations and found to be highly poly
Autor:
James W. Hainer, Kenneth J. Livak
Publikováno v:
Human Mutation. 3:379-385
Genotype determination using the solid-phase minisequencing method of Syvanen et al. (1990, 1993) has been adapted for use with fluorescein-labeled dideoxynucleotides (F-ddNTPs). PCR is performed using one biotinylated primer and one unbiotinylated p
Autor:
Kenneth K. Kidd, Hubert H.M. Van Tol, Kenneth J. Livak, Cathy L. Barr, Jay B. Lichter, James L. Kennedy
Publikováno v:
Human Molecular Genetics. 2:767-773
The human dopamine D4 receptor contains a novel polymorphism within the putative third cytoplasmic loop of the protein. The polymorphism is characterized by a varying number of direct imperfect 48-bp repeats in the gene. Pharmacological characterizat
Autor:
Yu. Ya. Shevelyov, Dmitry I. Nurminsky, Vladimir A. Gvozdev, Kenneth J. Livak, M. D. Balakireva
Publikováno v:
Nucleic Acids Research. 20:3731-3736
Expression of the X-linked repeated Stellate (Ste) genes, which code for a protein with 38% similarity to the beta-subunit of casein kinase II, is suppressed by the Su(Ste) locus on the Y chromosome. The structure and evolution of the Y-linked repeat
Publikováno v:
Mutation research. 406(1)
Sequencing of a human DNA ligase I cDNA clone derived from HeLa cells revealed two unreported differences with the published sequence: a single base change and a three-base deletion. Both differences are in exon 6, and were analyzed by amplifying a s
A detection system based on the PCR has been developed for Escherichia coli strains which harbor the Shiga-like toxin genes. This quantitative detection system involves the 5'-->3' nuclease activity of Thermus aquaticus DNA polymerase, which cleaves
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ee0482924cf959e26dabab92c00d240f
https://europepmc.org/articles/PMC167901/
https://europepmc.org/articles/PMC167901/
Publikováno v:
PCR methods and applications. 4(6)
The 5' nuclease PCR assay detects the accumulation of specific PCR product by hybridization and cleavage of a double-labeled fluorogenic probe during the amplification reaction. The probe is an oligonucleotide with both a reporter fluorescent dye and
Autor:
Thomas L. Hunt, Rhonda M Jenkins, John R. Crouse, J. Greg Terry, Donna L Shand, Jill M Connell, Peter J Gillies, Kenneth J Livak, Hanna Zyruk, James W. Hainer
Publikováno v:
Clinical pharmacology and therapeutics. 56(1)
Intestinal cholesterol esterification by the enzyme acyl-CoA:cholesterol acyltransferase (ACAT) is a presumed prerequisite for cholesterol absorption. We evaluated the effect of a potent, poorly absorbed ACAT inhibitor (DuP 128: N'-(2,4-difluoropheny
Publikováno v:
Nucleic acids research. 18(22)
Molecular genetic maps are commonly constructed by analyzing the segregation of restriction fragment length polymorphisms (RFLPs) among the progeny of a sexual cross. Here we describe a new DNA polymorphism assay based on the amplification of random