Zobrazeno 1 - 10
of 66
pro vyhledávání: '"Tomi P. Mäkelä"'
Publikováno v:
Molecular and cellular biology. 38(11)
In cancer, oncogene activation is partly mediated by acquired superenhancers, which therefore represent potential targets for inhibition. Superenhancers are enriched for BRD4 and Mediator, and both BRD4 and the Mediator MED12 subunit are disproportio
Autor:
Emilia Kuuluvainen, Michelle Sahal Estimé, Essi Havula, Mika Rämet, Tomi P. Mäkelä, Heini Hakala, Ville Hietakangas
Publikováno v:
Journal of Biological Chemistry. 289:16252-16261
The Cdk8 (cyclin-dependent kinase 8) module of Mediator integrates regulatory cues from transcription factors to RNA polymerase II. It consists of four subunits where Med12 and Med13 link Cdk8 and cyclin C (CycC) to core Mediator. Here we have invest
Publikováno v:
EMBO reports. 14:741-747
Loss of primary cilia is frequently observed in tumour cells, including glioblastoma cells, and proposed to benefit tumour growth, but a causal link has not been established. Here, we show that CCRK (cell cycle-related kinase) and its substrate ICK (
Autor:
Timofey V. Tselykh, Tomi P. Mäkelä, Ying Yang, Katja Helenius, Heli K. J. Pessa, Mikko J. Frilander
Publikováno v:
Nucleic Acids Research
The relevance of serine 5 phosphorylation of RNA polymerase II carboxy-terminal domain during initiation has been difficult to determine in mammalian cells as no general in vivo Ser5 kinase has been identified. Here, we demonstrate that deletion of t
Publikováno v:
Molecular and Cellular Biology. 29:315-323
Mammalian Cdk7, cyclin H, and Mat1 form the kinase submodule of transcription factor IIH (TFIIH) and have been considered ubiquitously expressed elements of the transcriptional machinery. Here we found that Mat1 and Cdk7 levels are undetectable in ad
Autor:
Niklas Ekman, Marianne Tiainen, Tea Vallenius, Pekka Katajisto, Tomi P. Mäkelä, Kari Vaahtomeri, Anou Londesborough
Publikováno v:
Development. 135:2331-2338
Inactivation of the tumor suppressor kinase Lkb1 in mice leads to vascular defects and midgestational lethality at embryonic day 9-11 (E9-E11). Here, we have used conditional targeting to investigate the defects underlying the Lkb1(-/-) phenotype. En
Publikováno v:
Molecular and Cellular Biology. 27:6177-6182
The Cdk8 kinase and associated proteins form a nonessential transcriptional repressor module of the Mediator in the budding yeast Saccharomyces cerevisiae. Genetic analyses of this module have demonstrated functions ranging from environmental respons
Autor:
Tomi P. Mäkelä, Michael D. Schneider, Brett H. Graham, Derrick J. Rossi, Motoaki Sano, Min Xie, Fernando Scaglia, Noriaki Shimizu, Katja Helenius, Heinrich Taegtmeyer, Yasukatsu Izumi, Christopher R. Wilson, Lingyun Hu, E. Dale Abel, George E. Taffet, Robia G. Pautler, Sihem Boudina, Masanori Asakura, Anastasia Kralli, Hirotoshi Tanaka
Publikováno v:
Cell Metabolism. 5:129-142
The Cdk7/cyclin H/ménage-à-trois 1 (MAT1) heterotrimer has proposed functions in transcription as the kinase component of basal transcription factor TFIIH and is activated in adult hearts by Gq-, calcineurin-, and biomechanical stress-dependent pat
Autor:
Lina Udd, Jérôme Boudeau, Jose M. Lizcano, Simon A. Hawley, Olga Göransson, Rachel Toth, Tomi P. Mäkelä, Dario R. Alessi, D. Grahame Hardie, Maria Deak, Nick A. Morrice
Publikováno v:
The EMBO Journal. 23:833-843
We recently demonstrated that the LKB1 tumour suppressor kinase, in complex with the pseudokinase STRAD and the scaffolding protein MO25, phosphorylates and activates AMP-activated protein kinase (AMPK). A total of 12 human kinases (NUAK1, NUAK2, BRS
Autor:
Tomi P. Mäkelä, Tea Vallenius, Burkhard Scharm, Aino Vesikansa, Keijo Luukko, Reinhold Schäfer
Publikováno v:
Experimental Cell Research. 293:117-128
ALP, CLP-36 and RIL form the ALP subfamily of PDZ-LIM proteins. ALP has been implicated in sarcomere function in muscle cells in association with alpha-actinin. The closely related CLP-36 is predominantly expressed in nonmuscle cells, where it locali